Reevaluation of the phenol-sulfuric acid reaction for the estimation of hexoses and pentoses

Rao, Pragna; Pattabiraman, T. N.

doi:10.1016/0003-2697(89)90387-4

Cited by 260 publications

(122 citation statements)

References 10 publications

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“…As a control, the resin was treated with the same procedure described above but without curdlan. The coupled 1,3-␤-D-glucans to resins were quantified by the sulfuric acid-phenol method (42). The resins that bound more than 3 g of glucose/mg of resin were used for purification of Tenebrio 1,3-␤-Dglucan-binding protein.…”

Section: Methodsmentioning

confidence: 99%

Characterization and Properties of a 1,3-β-d-Glucan Pattern Recognition Protein of Tenebrio molitor Larvae That Is Specifically Degraded by Serine Protease during Prophenoloxidase Activation

Zhang

Cho

Kim

et al. 2003

Journal of Biological Chemistry

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Although many different pattern recognition receptors recognizing peptidoglycan and 1,3-␤-D-glucan have been identified in vertebrates and insects, the molecular mechanism of these molecules in the pattern recognition and subsequent signaling is largely unknown. To gain insights into the action mechanism of 1,3-␤-D-glucan pattern recognition protein in the insect prophenoloxidase (proPO) activation system, we purified a 53-kDa 1,3-␤-D-glucan recognition protein (Tm-GRP) to homogeneity from the hemolymph of the mealworm, Tenebrio molitor, by using a 1,3-␤-D-glucan affinity column. The purified protein specifically bound to 1,3-␤-Dglucan but not to peptidoglycan. Subsequent molecular cloning revealed that Tm-GRP contains a region with close sequence similarity to bacterial glucanases. Strikingly, two catalytically important residues in glucanases are replaced with other nonhomologous amino acids in Tm-GRP. The finding suggests that Tm-GRP has evolved from an ancestral gene of glucanases but retained only the ability to recognize 1,3-␤-D-glucan. A Western blot analysis of the protein level of endogenous Tm-GRP showed that the protein was specifically degraded following the activation of proPO with 1,3-␤-Dglucan and calcium ion. The degradation was significantly retarded by the addition of serine protease inhibitors but not by cysteine or acidic protease inhibitor. These results suggest that 1,3-␤-D-glucan pattern recognition protein is specifically degraded by serine protease(s) during proPO activation, and we propose that this degradation is an important regulatory mechanism of the activation of the proPO system.

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Section: Methodsmentioning

confidence: 99%

Characterization and Properties of a 1,3-β-d-Glucan Pattern Recognition Protein of Tenebrio molitor Larvae That Is Specifically Degraded by Serine Protease during Prophenoloxidase Activation

Zhang

Cho

Kim

et al. 2003

Journal of Biological Chemistry

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“…As a control, the resin was treated as described above but without curdlan. The amount of coupled 1,3-␤-D-glucans to resins were quantified by the sulfuric acid-phenol method (27). The resins that had coupled more than 3 g of glucose/mg of resin were used for further purification of Holotrichia 1,3-␤-D-glucan-binding protein.…”

Section: Preparation Of 13-␤-d-glucan-and Pgn-immobilizedmentioning

confidence: 99%

Peptidoglycan Recognition Proteins Involved in 1,3-β-D-Glucan-dependent Prophenoloxidase Activation System of Insect

Lee

Osaki

Lee

et al. 2004

Journal of Biological Chemistry

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“…The sulfate group positions in the polysaccharide were revealed by HSQC spectroscopy (Bruker). All chromatographic assays were conducted with the phenol-sulfuric acid detection method [21].…”

Section: General Methodsmentioning

confidence: 99%

Anticoagulant Activity and Structural Characterization of Polysaccharide from Abalone (Haliotis discus hannai Ino) Gonad

et al. 2016

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Abstract:In this study, we aimed at characterizing the structure and the anticoagulant activity of a polysaccharide fraction (AGP33) isolated from the gonads of Haliotis discus hannai Ino. AGP33 was extracted by enzymatic hydrolysis and purified by ion-exchange and gel-filtration chromatography. The backbone fraction of AGP33 (BAGP33), which appeared to contain of mannose, glucose and galactose, was prepared by partial acid hydrolysis. According to methylation and nuclear magnetic resonance (NMR) spectroscopy, the backbone of AGP33 was identified as mainly consisting of 1Ñ3-linked, 1Ñ4-linked, and 1Ñ6-linked monosaccharides. AGP33 is a sulfated polysaccharide with sulfates occur at 3-O-and 4-O-positions. It prolonged thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT) compared to a saline control solution in a dosage-dependent manner. AGP33 exhibited an extension (p < 0.01) of APTT compared to the saline group at concentrations higher than 5 µg/mL. AGP33 exhibited higher anticoagulant activity than its desulfated product (AGP33-des) and BAGP33. The results showed that polysaccharide with higher molecular weight and sulfate content demonstrated greater anticoagulant activity.

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Reevaluation of the phenol-sulfuric acid reaction for the estimation of hexoses and pentoses

Cited by 260 publications

References 10 publications

Characterization and Properties of a 1,3-β-d-Glucan Pattern Recognition Protein of Tenebrio molitor Larvae That Is Specifically Degraded by Serine Protease during Prophenoloxidase Activation

Characterization and Properties of a 1,3-β-d-Glucan Pattern Recognition Protein of Tenebrio molitor Larvae That Is Specifically Degraded by Serine Protease during Prophenoloxidase Activation

Peptidoglycan Recognition Proteins Involved in 1,3-β-D-Glucan-dependent Prophenoloxidase Activation System of Insect

Anticoagulant Activity and Structural Characterization of Polysaccharide from Abalone (Haliotis discus hannai Ino) Gonad

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