“…Studies of reference genes in insects are common. In coleoptera, reference gene screening has been performed in approximately twenty insect species, such as Dendroctonus valens , Harmonia axyridis , and Cerambycidae species , Monochamus alternatus, Anoplophora glabripennis ( Toutges et al, 2010 ; Rajarapu et al, 2012 ; Shi et al, 2013 ; Barros Rodrigues et al, 2014 ; Wang et al, 2014 ; Song et al, 2015 ; Tan et al, 2015 ; Feng et al, 2016 ; Rodrigues et al, 2017 ; Yang et al, 2018 ; Yang et al, 2020 ; Zhang et al, 2020 ; Zheng et al, 2020 ; Guo et al, 2021 ; Sellamuthu et al, 2021 ). And common reference genes for Coleoptera studies include ACT (actin), β-TUB (beta-tubulin), α-TUB (alpha-tubulin), RPs (ribosomal proteins), 18S rRNA (18S ribosomal RNA), 28S rRNA (28S ribosomal RNA), EF1-α (elongation factor1-α) and so forth ( Toutges et al, 2010 ; Zhang et al, 2020 ; Guo et al, 2021 ).…”