Reference interval for immature platelet fraction on Sysmex XN hematology analyzer: a comparison study with Sysmex XE-2100

Abstract

Recently introduced hematology analyzer, the Sysmex XN modular system (Sysmex, Kobe, Japan), has newly adopted a florescent channel to detect platelets and immature platelet fraction (IPF). This study aimed to establish new reference intervals for %-IPF and absolute number of IPF (A-IPF) on Sysmex XN. Platelet counts, %-IPF, and A-IPF were also compared between Sysmex XN and XE-2100 systems (Sysmex).Except outliers, blood samples from 2104 healthy individuals and 140 umbilical cord blood were analyzed using both Sysmex XN and XE-2100. The results of two systems were compared using Bland-Altman plot. The reference intervals for %-IPF and A-IPF were defined using non-parametric percentile methods according to the Clinical and Laboratory Standard Institute guideline (C28-A3).The platelet counts, %-IPF, and A-IPF showed non-parametric distributions. The mean difference between Sysmex XN and XE-2100 in healthy individuals revealed a positive bias in platelets (+8.0×10This large-scale study demonstrates a clear difference of platelet counts and IPF between Sysmex XN and XE-2100. The new reference intervals for IPF on Sysmex XN would provide fundamental data for clinical practice and future research.

The immature platelet fraction: creating neonatal reference intervals and using these to categorize neonatal thrombocytopenias

“…Ko et al ., 5 , 6 from South Korea, recently reported IPF reference intervals using Sysmex XN and XE-2100 hematology analyzers. They observed similar IPF ranges for healthy adults and for umbilical cord blood obtained at full-term deliveries ( n = 120).…”

Innovative haematological parameters for early diagnosis of sepsis in adult patients admitted in intensive care unit

“…The results of our study show that the diagnostic performance of IPF# and RET%, two innovative parameters that can be measured with Sysmex XN module, is as good as that of CRP for predicting the risk of developing sepsis during ICU stay. Despite the excellent diagnostic performance of IPF# has been previously highlighted using the former generation of Sysmex analyzers (ie, the XE series),12–15 no information has been published for the XN module, the analytical performance and reference range of which do not completely overlap with those of the XE series 24. CRP is the most widely used laboratory test for diagnosing and monitoring localised and systemic infections.…”

Reference Intervals of Platelets, Lymphocytes and Cardiac Biomarkers in Umbilical Cord Blood

“…Table 1 shows the comparison of studies measuring IPF in healthy subjects. The new automated hematology analyzer, XN modular system, demonstrated remarkable higher and broader reference intervals for platelets and IPF compared with XE-2100 [36]. For these diferences, clinical laboratories should establish or verify reference intervals for platelets and IPF according to their own instrument.…”