Reference intervals for serum amyloid A, haptoglobin, ceruloplasmin, and fibrinogen in apparently healthy neonatal lambs

Dinler, Ceren; Tuna, Gülten Emek; Ay, Erhan; Ulutaş, Bülent; Voyvoda, Hüseyin; Ulutaş, Pınar Alkım

doi:10.1111/vcp.12883

Cited by 7 publications

(11 citation statements)

References 31 publications

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“…Another study reported that serum SAA concentration increased from the first day of life to the fifth day, the highest level was on the day after birth and then decreased until the second week of life (Kilpi, 2015). In some studies which were conducted on sheep and goats, the researchers found that the SAA and Hp concentrations in healthy animals were quite low in the APP examination (Iliev and Georgieva, 2018;Dinler et al 2020). Similarly in our study, SAA and Hp concentrations of post-treatment and control groups were lower than the concentrations of pre-treatment.…”

Section: Resultssupporting

confidence: 76%

“…Also, the fact that SAA and Hp concentrations were in close values to each other in control and post-treatment groups is another indicator of the success of the treatment. To conclude, the fact that SAA and Hp concentrations were found to be low in healthy animals in some studies (Iliev and Georgieva, 2018;Dinler et al 2020) supports the hypothesis.…”

Section: Resultssupporting

confidence: 52%

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Evaluating the Efficacy of the Treatment in Lambs with Cryptosporidium spp. via Saa and Haptoglobin

Kabu¹,

Civelek²,

Tunç³

et al. 2023

IJAR

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Background: The purpose of this study is to evaluate the efficacy of the treatment for the diarrhea due to Cryptosporidium, which is prevalent in lambs, via the concentrations of Serum Amyloid A (SAA) and Haptoglobin (Hp). Although there are experimental studies in this field, there are quite a few studies on the lambs which are infected naturally. Methods: In this study, stool samples were collected from the lambs that were affected with diarrhea and Cryptosporidium was detected via the rapid test kit (Bio-X Diagnostics S.A. Rochefort/Belgium). Following this procedure, 15 lambs were included in the study by examining same stool samples microscopically and 50 mg/kg of paromomycin sulphate (Huvepharma) was administered to the lambs in which Cryptosporidium spp. was detected for five days as a treatment. 10 healthy lambs formed the control group. Blood samples were taken from all lambs to measure SAA and Hp in the pre and post treatment stages. In the serums obtained from blood samples, concentrations of SAA and Hp were measured in ELISA by using commercial kits. Result: In the lambs with Cryptosporidium, concentrations of SAA and HP were as (SAA; 20,62±4,83 ng/mL HP; 2,63±0,77 µg/mL) in pre-treatment and as (SAA;12,93±3,27 ng/mL, Hp; 1,59±0,73 µg/mL) in post-treatment stage while they were as (SAA;10,07±2,49 ng/mL, Hp; 1,03±0,59 µg/mL) in the control group. In the pre and post treatment stage measurements, a statistically significant difference (p less than 0,05) in the values of SAA and Hp was found between the control group and the lambs wit diarrhea due to Cryptosporidium. It was suggested that this might have occurred as a result of inflammatory response against Cryptosporidium. In the light of these findings, it is asserted that routine measurements of SAA and Hp concentrations of the lambs with diarrhea due to cryptosporidiosis could be beneficial in the following up the treatment, determining the severity of the infection, choosing the right method for treatment and monitoring the efficacy of the chosen treatment method and in detecting subclinical diseases in veterinary medicine.

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Section: Resultssupporting

confidence: 76%

Section: Resultssupporting

confidence: 52%

Evaluating the Efficacy of the Treatment in Lambs with Cryptosporidium spp. via Saa and Haptoglobin

Kabu¹,

Civelek²,

Tunç³

et al. 2023

IJAR

View full text Add to dashboard Cite

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“…How general are these findings? Perinatal levels of acute phase proteins have been observed to be low in several species of mammal, for instance rabbits, humans and sheep (Baker and Long, 1990;Kanakoudi et al, 1995;Rygg et al, 1996;Liberatori et al, 1997;Mcnamara and Alcorn, 2002;Mithal et al, 2017;Dinler et al, 2020), but to our knowledge this is the first evidence of a similar trend in a Carnivoran. The unique feature in grey and potentially other phocid seals is that this applies where protection against infection by antibodies may be initially non-existent and then minimal until parental care and lactational support ceases.…”

Section: Acute Phase Proteins-paucity In Pupsmentioning

confidence: 79%

Is a Little Enough? Paucity of Immune Proteins in Serum of Precocial Neonates of a Marine Carnivoran—the Atlantic Grey Seal

et al. 2022

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Mammalian mothers usually provide their offspring with large quantities of immunoglobulins (antibodies) for circulation in blood, either trans-placentally before birth, via colostrum briefly thereafter, or, less commonly, from milk. Neonates of true, phocid seals, however, are peculiarly impoverished in serum immunoglobulins, the levels of which slowly increase but do not reach adult levels by the time of weaning. We investigated whether grey seal (Halichoerus grypus) neonates compensate through an elevation or rapid maturation in levels of serum innate immune factors, namely acute phase and complement proteins. Instead, their sera contained remarkably low levels of acute phase proteins (including C-reactive protein, haptoglobin, hemopexin, ceruloplasmin, orosomucoid), compared to their mothers, that barely increased to adult levels by weaning. For complement, there was a strong demarcation between the early activation and amplification cascade components (present at normal adult levels in pups) and the late lytic membrane attack complex and regulatory proteins (consistently at low relative levels). Phocid neonates therefore differ dramatically from land Carnivorans, such as dogs and cats, in early life immune protection. That neonatal phocids survive this apparent vulnerability to infections between birth and weaning prompts questions as to what other mechanisms protect them, and the adaptive value of their seeming vulnerability.

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“…The concentrations of different APP change during the first few weeks of ruminant life and are termed age-related changes in APP. In lambs and goat kids, SAA concentrations increase during the first week of life, start to decrease during the second week, and then stabilize at the end of the third week (Eckersall et al, 2008;Ulutas et al, 2017;Niine et al, 2018a;Peetsalu et al, 2019;Dinler et al, 2020). It has been suggested that colostrum intake influences the APP concentration in calves, as their SAA concentrations were found to be low before colostrum consumption and then increased during the first 24 h after birth (Orro et al, 2008;Tóthová et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Effect of colostrum on the acute-phase response in neonatal dairy calves

Peetsalu

Niine

Loch

et al. 2022

Journal of Dairy Science

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The core part of the mammal innate immune system is the acute-phase response (APR), during which acutephase proteins (APP) are synthesized. Colostrum contains immunomodulating factors such as proinflammatory cytokines and APP in large quantities. We looked at proinflammatory cytokines [IL-1β, IL-6, and tumor necrosis factor-α (TNF-α)] and APP [serum amyloid A (SAA) and haptoglobin (Hp)] in colostrum and in calves' serum. The aim of this study was to evaluate the effects of colostrum on the calves' systemic APR and the associations of the calves' serum APR with short-and long-term weight gain (at the age of 1, 3, and 9 mo). A total of 143 female dairy calves were studied during their first 3 wk of life. The calves were separated from their mothers immediately after birth and bottle-fed 3 L of quality-controlled colostrum once within 2 h after birth. Serum samples were collected once a week during the first 3 wk of life (a total of 1-3 samples per calf). Mean sampling age (±standard deviation) was 4.3 (±2.0) d in the first week, 11.0 (±2.0) d in the second week, and 18.0 (±2.0) d in the third week. Linear regression models were used to study associations of colostrum APP and cytokine concentration with serum APR markers and for studying associations of colostrum and serum APR markers with calves' average daily weight gain (ADWG). Mixed linear regression models were used to compare serum concentrations of APR markers by study weeks. The colostrum IL-6 concentrations were positively associated with serum IL-6 in the first 3 wk of life. Colostrum IL-1β was positively associated with calves' serum IL-1β during the first week of life, and colostrum TNF-α was positively associated with calves' serum TNF-α during the first 2 wk of life. Serum IL-1β concentrations differed over the 3 wk, being the highest during the first week and the lowest during the second week. For IL-6, the concentration during the first week was the highest, and for TNF-α, a steady decline in the concentration was observed. Serum SAA concentrations were elevated during the first 2 wk of life and subsequently declined during the third week. Albumin concentrations were lowest in the first week, whereas Hp concentrations were highest during the second week. Serum concentrations of SAA, Hp, IL-6, and TNF-α during the second week were negatively associated with ADWG at 9 mo of age. The SAA concentrations during the third week of age had a negative association with 9-mo ADWG. Serum Hp concentrations in the third week were negatively associated with 3-mo ADWG. The results of our study suggest that colostrum cytokines influence calf serum cytokine concentrations. Thus, they influence the newborn calves' adaptation to the environment and the development of their immune system. Factors that activate an APR during the second and third week of life have a long-term influence on calves' development.

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Reference intervals for serum amyloid A, haptoglobin, ceruloplasmin, and fibrinogen in apparently healthy neonatal lambs

Cited by 7 publications

References 31 publications

Evaluating the Efficacy of the Treatment in Lambs with Cryptosporidium spp. via Saa and Haptoglobin

Evaluating the Efficacy of the Treatment in Lambs with Cryptosporidium spp. via Saa and Haptoglobin

Is a Little Enough? Paucity of Immune Proteins in Serum of Precocial Neonates of a Marine Carnivoran—the Atlantic Grey Seal

Effect of colostrum on the acute-phase response in neonatal dairy calves

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