Reference reagents for antinuclear antibodies

Tan, Eng M.; Feltkamp, T. E. W.; Alarcón‐Segovia, Donato; Dawkins, Roger L.; Homma, Mitsuo; Kalden, J. R.; Lambert, Paul‐Henri; Lange, Anna; Maini, R. N.; McDuffie, Frederic C.; McDougal, J. Steven; R, Norberg; Wilson, Merlin R.

doi:10.1002/art.1780311019

Cited by 34 publications

(11 citation statements)

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“…Control sera with anti-SS-A/Ro autoantibodies from the Center for Disease Control/Arthritis Foundation (CDC/AF) serum bank (33,34), as well as previously characterized sera from the Advanced Diagnostics Laboratory were used in this study (35). All sera were retested to confirm the discrepant results, aliquoted to avoid repetitive freeze-thaw cycles, and then stored undiluted at -20 or -70°C until required for assays.…”

Section: Seramentioning

confidence: 99%

Specificity of autoantibodies to SS‐A/Ro on a transfected and overexpressed human 60 kDa Ro autoantigen substrate

Fritzler

Hanson

Miller

et al. 2002

Clinical Laboratory Analysis

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The objective of this study was to analyze apparently discrepant results that arose during the use of an indirect immunofluorescence (IIF) assay using transfected HEp-2 cells to detect anti-SS-A/Ro autoantibodies in human sera. Fourteen sera that had SS-A/Ro antibodies as detected on this commercial substrate, but did not have antibodies to SS-A/Ro as determined by double immunodiffusion (ID) or enzyme-linked immunosorbent assay (ELISA), were studied by immunoprecipitation (IP) of radiolabeled cell extracts and full-length recombinant SS-A/Ro. A multi-antigen strip immunoblotting (IB) assay containing both the 52- and 60-kDa antigens was included in the analysis. We confirmed that 12 of 14 of the sera under study had antibodies to SS-A/Ro protein antigens as determined by at least one other immunoassay. One serum had antibodies to hyRNA but no detectable reactivity with the 52- or 60-kDa antigens. One serum remained negative in all assays for SS-A/Ro autoantibodies.

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Section: Seramentioning

confidence: 99%

Specificity of autoantibodies to SS‐A/Ro on a transfected and overexpressed human 60 kDa Ro autoantigen substrate

Fritzler

Hanson

Miller

et al. 2002

Clinical Laboratory Analysis

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“…Seven of the 9 complied with this request. From these results, together with those obtained previously (7,9), a consensus was established regarding the specific autoantibody profiles of the reference sera; this is shown in Table 2. Operating characteristics (sensitivity and specificity) of the various kits were determined relative to these standards.…”

Section: Tan Et Almentioning

confidence: 99%

“…The ANA Subcommittee of the International Union of Immunological Societies (IUIS) Standardization Committee has been instrumental in establishing a bank of 10 different sera that have been designated as reference reagents for autoantibodies of different antigenic specificities (8)(9)(10). These reference reagents, containing antibodies of defined specificities, can be used to critically evaluate the performance of commercial EIA test kits that are marketed for the detection of single antigen-antibody systems.…”

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confidence: 99%

A critical evaluation of enzyme immunoassays for detection of antinuclear autoantibodies of defined specificities: I. Precision, sensitivity, and specificity

Tan

Smolen

McDougal

et al. 1999

Arthritis & Rheumatism

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167

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Objective. To determine the performance characteristics of enzyme-based immunoassay (EIA) kits for the detection of antinuclear and other autoantibodies of defined specificities. Methods. Nine manufacturers of EIA kits to detect antibodies of defined specificities participated in a study in which they received coded sera from the Centers for Disease Control and Prevention. These coded sera contained different dilutions of antibody of one specificity mixed with sera containing antibodies of other specificities. The manufacturers were asked to use their standard technology to determine antibody content and send the data to a committee of the International Union of Immunological Societies for analysis. The data were analyzed for sensitivity and specificity in the detection of anti-double-stranded DNA (anti-dsDNA), anti-single-stranded DNA, antihistone, anti-Sm, anti-U1 RNP, anti-SSA/Ro, anti-SSB/La, anti-Scl-70 (DNA topoisomerase I), anticentromere, and anti-Jo-1 antibodies. In addition, replicate samples were included in the coded sera to evaluate the precision of each EIA method. Results. Lack of sensitivity and specificity was most evident in the anti-dsDNA and anti-Sm kits, although 2 kits for anti-dsDNA achieved acceptable sensitivity and specificity. Generally, anti-SSA/Ro, anti-SSB/La, anti-Scl-70, anticentromere, and anti-Jo-1 kits performed well. Many false-positive results were obtained with a multiple myeloma serum containing cryo-precipitates, but multiple myeloma sera without cryo-precipitates presented no problem in the EIA system. Precision, based on evaluation of replicate samples, varied from very good to poor. Conclusion. No single manufacturer was clearly superior to others in terms of their products' overall

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“…Samples of cell extracts were first digested with various concentrations of S.a.V8 protease for 30 min at 37°C and then immunoprecipitated with anti-SS-B/La serum Ze, the Centers for Disease Control reference serum (27). This anti-SS-B/La serum was known to contain antibodies which were reactive with both domains X and Y (4).…”

mentioning

confidence: 99%

The small nuclear ribonucleoprotein SS-B/La binds RNA with a conserved protease-resistant domain of 28 kilodaltons.

Chan¹,

Tan²

1987

Mol. Cell. Biol.

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Reference reagents for antinuclear antibodies

Cited by 34 publications

References 0 publications

Specificity of autoantibodies to SS‐A/Ro on a transfected and overexpressed human 60 kDa Ro autoantigen substrate

Specificity of autoantibodies to SS‐A/Ro on a transfected and overexpressed human 60 kDa Ro autoantigen substrate

A critical evaluation of enzyme immunoassays for detection of antinuclear autoantibodies of defined specificities: I. Precision, sensitivity, and specificity

The small nuclear ribonucleoprotein SS-B/La binds RNA with a conserved protease-resistant domain of 28 kilodaltons.

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