Refined structure of 1,2-diacyl-P-O-ethylphosphatidylcholine bilayer membranes

Winter, Ingrid; Pabst, Georg; Rappolt, Michael; Lohner, Karl

doi:10.1016/s0009-3084(01)00172-4

Cited by 38 publications

(51 citation statements)

References 53 publications

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“…Interdigitation has been described for cationic lipids presenting large headgroups and short acyl chains or high surface charge density (Pohle et al 2000;Lewis et al 2001;Winter et al 2001;Ryhanen et al 2005). In those cases, the electrostatic repulsion between headgroups played a major role, since addition of neutral lipids or salt affected interdigitation (Ryhanen et al 2005).…”

Section: Different Gel Bilayer Structures: Interdigitationmentioning

confidence: 99%

Structural insights on biologically relevant cationic membranes by ESR spectroscopy

et al. 2017

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Cationic bilayers have been used as models to study membrane fusion, templates for polymerization and deposition of materials, carriers of nucleic acids and hydrophobic drugs, microbicidal agents and vaccine adjuvants. The versatility of these membranes depends on their structure. Electron spin resonance (ESR) spectroscopy is a powerful technique that employs hydrophobic spin labels to probe membrane structure and packing. The focus of this review is the extensive structural characterization of cationic membranes prepared with dioctadecyldimethylammonium bromide or diC14-amidine to illustrate how ESR spectroscopy can provide important structural information on bilayer thermotropic behavior, gel and fluid phases, phase coexistence, presence of bilayer interdigitation, membrane fusion and interactions with other biologically relevant molecules.

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Section: Different Gel Bilayer Structures: Interdigitationmentioning

confidence: 99%

Structural insights on biologically relevant cationic membranes by ESR spectroscopy

et al. 2017

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“…Fatty acyl chain interdigitation can be introduced by spacing the head groups apart a bit further, e.g., by going from a negatively charged phosphate diester to a neutral phosphate triester [26]. In the case of diC14-amidine one extra CH 2 atom in the backbone is thought to space the tails apart a bit more than natural, leading to an ease of chain interdigitation [27].…”

Section: Interdigitationmentioning

confidence: 99%

Phase behavior of selected artificial lipids

Dobner

Brezesinski

2014

Current Opinion in Colloid & Interface Science

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“…The difference in environment encountered by the probe in the two cases may therefore be due to variation in hydration in the two membranes owing to different charges [19,40]. It is not clear whether this factor plays any role in the ability of cationic lipids such as EPOPC as transfection agents [30] and represents an interesting question.…”

Section: Discussionmentioning

confidence: 99%

“…This could be due to decreased water content of EPOPC membranes compared to POPC membranes. In fact, it has previously been shown that the ethylphosphatidylcholine derivative for a series of saturated chain lipids has significantly reduced water content than the corresponding unesterified phosphatidylcholines in the fluid state [19,40].…”

Section: Time-resolved Fluorescence Measurementsmentioning

confidence: 99%

Modulation of Fluorophore Environment in Host Membranes of Varying Charge

Kelkar

Ghosh

Chattopadhyay

2003

Journal of Fluorescence

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The net electrical charge of the biological membrane represents an important parameter in the organization, dynamics and function of the membrane. In this paper, we have characterized the change in the microenvironment experienced by a membrane-bound fluorescent probe when the charge of the phospholipids constituting the host membrane is changed from zwitterionic to cationic with minimal change in the chemical structure of the host lipid. In particular, we have explored the difference in the microenvironment experienced by the fluorescent probe 2-(9-anthroyloxy)stearic acid (2-AS) in model membranes of zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and cationic 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine (EPOPC) which are otherwise chemically similar, using the wavelength-selective fluorescence approach and other fluorescence parameters. Our results show that the microenvironment experienced by a membrane probe such as 2-AS is different in POPC and EPOPC membranes, as reported by red edge excitation shift (REES) and other fluorescence parameters. The difference in environment encountered by the probe in the two cases could possibly be due to variation in hydration in the two membranes owing to different charges.

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Refined structure of 1,2-diacyl-P-O-ethylphosphatidylcholine bilayer membranes

Cited by 38 publications

References 53 publications

Structural insights on biologically relevant cationic membranes by ESR spectroscopy

Structural insights on biologically relevant cationic membranes by ESR spectroscopy

Phase behavior of selected artificial lipids

Modulation of Fluorophore Environment in Host Membranes of Varying Charge

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