Refinement of an open-microcavity optical biosensor for bacterial endotoxin test

Akimov, Nikolay; Scudder, Jonathan; Ye, Jing Yong

doi:10.1016/j.bios.2021.113436

Cited by 16 publications

(5 citation statements)

References 39 publications

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“…To evaluate the selectivity, the aptasensor was challenged with the discrimination of endotoxin against the non-target substances. As the presence of thrombin, polynucleotides, glucans, and so forth can result in a positive response to the standard LAL-based assay, 46 the selectivity was tested using thrombin, lectin, carcinoembryonic antigen (CEA), human serum albumin (HSA), α-fetoprotein (AFP), adenosine 5′-triphosphate (ATP), and dextran as the interfering substances. In the proof-of-concept experiment, the concentration of endotoxin was 0.25 ng/mL, while for other substances, it was 1.0 ng/mL.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Dually Amplified Electrochemical Aptasensor for Endotoxin Detection via Target-Assisted Electrochemically Mediated ATRP

Wan

Liang

et al. 2023

Anal. Chem.

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As the entering of bacterial endotoxin into blood can cause various life-threatening pathological conditions, the screening and detection of low-abundance endotoxin are of great importance to human health. Taking advantage of signal amplification by target-assisted electrochemically mediated atom transfer radical polymerization (teATRP), we illustrate herein a simple and cost-effective electrochemical aptasensor capable of detecting endotoxin with high sensitivity and selectivity. Specifically, the aptamer receptor was employed for the selective capture of endotoxin, of which the glycan chain was then decorated with ATRP initiators via covalent coupling between the diol sites and phenylboronic acid (PBA) group, followed by the recruitment of ferrocene signal reporters via the grafting of polymer chains through potentiostatic eATRP under ambient temperature. As the glycan chain of endotoxin can be decorated with hundreds of ATRP initiators while the further grafting of polymer chains through eATRP can recruit hundreds to thousands of signal reporters to each initiator-decorated site, the teATRP-based strategy allows for the dual amplification of the detection signal. This dually amplified electrochemical aptasensor has the ability to sensitively and selectively detect endotoxin at a concentration as low as 1.2 fg/ mL, and its practical applicability has been further demonstrated using human serum samples. Owing to the simplicity, high efficiency, biocompatibility, and inexpensiveness of the teATRP-based amplification strategy, this electrochemical aptasensor holds great application potential in the sensitive and selective detection of low-abundance endotoxin and many other glycan chaincontaining bio-targets.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Dually Amplified Electrochemical Aptasensor for Endotoxin Detection via Target-Assisted Electrochemically Mediated ATRP

Wan

Liang

et al. 2023

Anal. Chem.

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“…And presence of glucose can lead to false-positive results, greatly limiting its application in the biomedical field. Therefore, biosensors independent of Limulus reagent have been widely studied. − Wang et al developed a colorimetric and fluorometric probe based on 3-phenylthiophene-based water-soluble copolythiophenes, with the emission wavelength of 600 nm, which can detect LPS at the picomolar level . Liedberg et al developed an LPS sensor by assembling tetramethylrhodamine-labeled LPS-binding peptides on graphene oxide, which can be used as a turn-on fluorescence probe .…”

Section: Introductionmentioning

confidence: 99%

Near-Infrared-Fluorescent Probe for Turn-On Lipopolysaccharide Analysis Based on PEG-Modified Gold Nanorods with Plasmon-Enhanced Fluorescence

Yang

Hou

et al. 2021

ACS Appl. Mater. Interfaces

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Lipopolysaccharide (LPS), as the major component of the outer membrane of Gram-negative bacteria, can trigger a variety of biological effects such as sepsis, septic shock, and even multiorgan failure. Herein, we developed a near-infrared-fluorescent probe for fluorescent turn-on analysis of LPS based on plasmon-enhanced fluorescence (PEF). Gold nanorods (Au NRs) modified polyethylene glycol (PEG) was used as PEF materials. Au NRs were prepared with different longitudinal surface plasmon resonance (LSPR), and their fluorescence enhancement was investigated. Three kinds of molecular weights (1000, 5000, and 10000) of polyethylene glycol (PEG) were employed to control the distance between the Au NRs and the fluorescence substances of cyanine 7 (Cy7). Experimental analysis showed that the enhancement was related to the spectral overlap between the plasmon resonance of Au NRs and the extinction/emission of fluorophore. The three-dimensional finite-difference time-domain (3D-FDTD) simulation further revealed that the enhancement was caused by local electric field enhancement. Furthermore, the probe was used for the ultrasensitive analysis of LPS with a detection limit of 3.85 ng/mL and could quickly distinguish the Gram-negative bacterium-Escherichia coli (E. coli) (with LPS in the membrane) from Gram-positive bacterium-Staphylococcus aureus (S. aureus) (without LPS), as well as quantitative determination of E. coli with a detection limit of 1.0 × 106 cfu/mL. These results suggested that the prepared probe has great potential for biomedical diagnosis and selective detection of LPS from different bacterial strains.

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“…The standard method for LPS detection is the Limulus amebocyte lysate (LAL) test. 6 This method is simple, rapid, and sensitive. However, the presence of many other components such as thromboplastin, thrombin, glucans, and polynucleotides can result in a positive response.…”

mentioning

confidence: 99%

“…The standard method for LPS detection is the Limulus amebocyte lysate (LAL) test . This method is simple, rapid, and sensitive.…”

mentioning

confidence: 99%

Boronate Affinity-Amplified Electrochemical Aptasensing of Lipopolysaccharide

Feng

Liang

et al. 2022

Anal. Chem.

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As lipopolysaccharide (LPS) is closely associated with sepsis and other life-threatening conditions, the point-of-care (POC) detection of LPS is of significant importance to human health. In this work, we illustrate an electrochemical aptasensor for the POC detection of low-abundance LPS by utilizing boronate affinity (BA) as a simple, efficient, and cost-effective amplification strategy. Briefly, the BA-amplified electrochemical aptasensing of LPS involves the tethering of the aptamer receptors and the BA-mediated direct decoration of LPS with redox signal tags. As the polysaccharide chain of LPS contains hundreds of cis-diol sites, the covalent crosslinking between the phenylboronic acid group and cis-diol sites can be harnessed for the site-specific decoration of each LPS with hundreds of redox signal tags, thereby enabling amplified detection. As it involves only a single-step operation (∼15 min), the BA-mediated signal amplification holds the significant advantages of unrivaled simplicity, rapidness, and cost-effectiveness over the conventional nanomaterial- and enzyme-based strategies. The BA-amplified electrochemical aptasensor has been successfully applied to specifically detect LPS within 45 min, with a detection limit of 0.34 pg/mL. Moreover, the clinical utility has been validated based on LPS detection in complex serum samples. As a proof of concept, a portable device has been developed to showcase the potential applicability of the BA-amplified electrochemical LPS aptasensor in the POC testing. In view of its simplicity, rapidness, and cost-effectiveness, the BA-amplified electrochemical LPS aptasensor holds broad application prospects in the POC testing.

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Refinement of an open-microcavity optical biosensor for bacterial endotoxin test

Cited by 16 publications

References 39 publications

Dually Amplified Electrochemical Aptasensor for Endotoxin Detection via Target-Assisted Electrochemically Mediated ATRP

Dually Amplified Electrochemical Aptasensor for Endotoxin Detection via Target-Assisted Electrochemically Mediated ATRP

Near-Infrared-Fluorescent Probe for Turn-On Lipopolysaccharide Analysis Based on PEG-Modified Gold Nanorods with Plasmon-Enhanced Fluorescence

Boronate Affinity-Amplified Electrochemical Aptasensing of Lipopolysaccharide

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