1987
DOI: 10.1016/0022-2836(87)90250-6
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Refinement of the C2221 crystal form of oxidized uteroglobin at 1.34 Å resolution

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Cited by 123 publications
(62 citation statements)
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“…However, the results of our binding assays fail to support this assumption in that only H-tag-rhUG but not rhUG without the H-tag can bind 45 Ca þþ . Furthermore, the results of structural studies with rabbit and human UGs did not reveal a Ca þþ -binding motif in this protein [25][26][27][28][29]. Thus, the results of our studies demonstrating Lys 43 and Asp 46 in rhUG to be crucial for its sPLA 2 -inhibitory activity and that rhUG without the H-tag fails to bind Ca þþ , it is highly unlikely that sequestration of Ca þþ by this protein is one of the mechanisms by which hUG inhibits sPLA 2 activity.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…However, the results of our binding assays fail to support this assumption in that only H-tag-rhUG but not rhUG without the H-tag can bind 45 Ca þþ . Furthermore, the results of structural studies with rabbit and human UGs did not reveal a Ca þþ -binding motif in this protein [25][26][27][28][29]. Thus, the results of our studies demonstrating Lys 43 and Asp 46 in rhUG to be crucial for its sPLA 2 -inhibitory activity and that rhUG without the H-tag fails to bind Ca þþ , it is highly unlikely that sequestration of Ca þþ by this protein is one of the mechanisms by which hUG inhibits sPLA 2 activity.…”
Section: Discussionmentioning
confidence: 99%
“…The results of these and other studies [27,28] showed that rabbit and human UGs are indistinguishable proteins both structurally [29] and functionally [23].…”
mentioning
confidence: 86%
“…1). Previously, we reported that recombinant rabbit and hUGs , Mantile et al, 1993 are virtually identical both structurally Morize et al, 1987) and functionally Mantile et al, 1993). The many and varied biological properties of UG and hUG described so far are suggested to be related to either their PLA2-inhibitory property (Levin et al, 1986;Miele et al, 1988;Singh et al, 1988a,b;Miele et al, 1990;Facchiano et al, 1991;Mantile et al, 1993) or due to their ability to bind various xenobiotic agents (Beato, 1976(Beato, , 1977Fridlansky and Milgrom, 1976;Atger et al, 1980;Bochskenal and Kirchner, 1981;Gillener, 1988;Nordlund-Moller er al, 1990;Peter et al, 1992; Lopez de Haro and Neato, 1994).…”
Section: Profile Of Patients With Atopic Asthma Andmentioning
confidence: 87%
“…Uteroglobin (UG) is a homodimer containing two disulfide bonds in its quaternary structure that covalently connect the two Almost three decades ago, two laboratories independently monomers of 70 amino acids each, which are antiparallel in oridiscovered a steroid-dependent secretory protein in the entation Morize et al, 1987; Bally and rabbit uterus during early pregnancy that was named blastokinin Delettre, 1989). Each monomer has four a-helices and one /3-turn (Krishnan and Daniel, 1967) or uteroglobin (Beier, 1968).…”
Section: Introductionmentioning
confidence: 99%
“…The resolution of this structure was improved to 134 pm [13], which enabled the characterization of the possible molecular basis of ligand binding, although a complex of UG with a ligand has not yet been crystallized.…”
mentioning
confidence: 99%