Reflectance confocal microscopy of solitary mastocytoma and correlation with horizontal histopathological sections

Correlation Between In Vivo Reflectance Confocal Microscopy and Horizontal Histopathology in Skin Cancer: A Review

“…( 39 ) first described RCM findings of mastocytosis in a huge group of 200 patients, including all different clinical presentation; regardless of the specific variant examined; all cases showed similar RCM features: the absence of aggregates of bright element in the context of finely granular and edematous papillary dermis was a constant finding. Following these results, our group first described more specific RCM features of SM and correlated them with HSS for validation ( 15 ): in particular, the presence of enlarged dermal papillae, containing tortuous vessels and large, uniformly round-shaped, bright cells at the level of dermoepidermal junction ( Figure 3A ) perfectly matched with the finding of aggregates of round, CD117-positive mastocytes with granular cytoplasm located to dermal papillae on HHS ( Figure 3B ).…”

Horizontal histopathology correlates with in vivo reflectance confocal microscopy features of molluscum contagiosum: A case series

“…[2][3][4][5][6][7][8] A strong correlation has been found in multiple inflammatory and neoplastic skin diseases, including psoriasis, discoid lupus erythematosus, mycosis fungoides, squamous cell carcinoma in situ, and others. [2][3][4][5][6][7] The aim of this paper was to correlate RCM findings of molluscum contagiosum (MC) to HHSs in order to confirm the use of RCM in the diagnostic approach to MC. Six patients (four males and two females; median age: 13.2 years) with multiple cutaneous lesions clinically and dermoscopically typical for MC were enrolled in the study.…”

“…For each patient, one lesion was examined using the handheld RCM device (Vivascope 3000, Caliber I.D., Rochester, New York), excised and processed for HHSs as previously described. [2][3][4][5][6][7] Slides were stained with hematoxylin and eosin and "digitalized" by using a Scan-Scope Digital Slide Scanner (Aperio, Vista, California). An informed consent was signed by all patients included in the study.…”

“…1 Although the resolution of RCM is almost comparable to that obtained by histopathology, these two techniques visualize the skin from different "angles," as RCM and traditional histopathology provide transverse and vertical sections of the skin, respectively. [2][3][4][5][6][7] In order to solve this discrepancy and to further support the diagnostic accuracy of RCM in skin disorders, our research group in the past years has confirmed the reliability of RCM by correlating its findings with horizontal histopathological sections (HHSs), which, unlike the traditional ones, reflect the same plane as RCM. [2][3][4][5][6][7][8] A strong correlation has been found in multiple inflammatory and neoplastic skin diseases, including psoriasis, discoid lupus erythematosus, mycosis fungoides, squamous cell carcinoma in situ, and others.…”

“…[2][3][4][5][6][7] In order to solve this discrepancy and to further support the diagnostic accuracy of RCM in skin disorders, our research group in the past years has confirmed the reliability of RCM by correlating its findings with horizontal histopathological sections (HHSs), which, unlike the traditional ones, reflect the same plane as RCM. [2][3][4][5][6][7][8] A strong correlation has been found in multiple inflammatory and neoplastic skin diseases, including psoriasis, discoid lupus erythematosus, mycosis fungoides, squamous cell carcinoma in situ, and others. [2][3][4][5][6][7] The aim of this paper was to correlate RCM findings of molluscum contagiosum (MC) to HHSs in order to confirm the use of RCM in the diagnostic approach to MC.…”