Refolding of autodisplayed anti-NEF scFv through oxidation with glutathione for immunosensors

Intracellular Imaging of Glutathione with MnO2 Nanosheet@Ru(bpy)32+–UiO-66 Nanocomposites

“…Therefore, efficient detection of GSH in living biosystems is favorable to grasp GSH-related pathophysiological events . Various methods including high-performance liquid chromatography, electrochemistry, colorimetry, photoelectrochemistry, surface-enhanced Raman spectroscopy, enzyme-linked immunosorbent, as well as fluorescent assays have exerted prospective assays for the detection of GSH . Among them, the fluorescent detection technique would be recommendable for the successful detection of GSH and intracellular imaging, owing to its nondestructive characteristics, real-time monitoring, rapid response, high selectivity and sensitivity, and the possibility of detection in a living system. − Recently, 2D-layered manganese dioxide nanosheets (MnO 2 NSs) have attracted much attention on the exploration of fluorescent GSH sensing systems due to its distinguished redox activity to recognize GSH and splendid fluorescent quenching ability toward luminescent fluorophores. , However, the reported fluorescent probes were usually directly conjugated on the coverage of the MnO 2 NS, exposing the probes to the exterior environment, which is prone to suffer from photobleaching and premature leakage and subsequently limits probe’s photostability and assay sensitivity .…”

“…4 Therefore, efficient detection of GSH in living biosystems is favorable to grasp GSH-related pathophysiological events. 5 Various methods including high-performance liquid chromatography, 6 electrochemistry, 7 colorimetry, 8 photoelectrochemistry, 9 surface-enhanced Raman spectroscopy, 10 enzyme-linked immunosorbent, 11 as well as fluorescent assays have exerted prospective assays for the detection of GSH. 12 Among them, the fluorescent detection technique would be recommendable for the successful detection of GSH 13 and intracellular imaging, 14 owing to its nondestructive characteristics, realtime monitoring, rapid response, high selectivity and sensitivity, and the possibility of detection in a living system.…”

A one-step immunoassay based on switching peptides for diagnosis of porcine epidemic diarrhea virus (PEDV) using screened Fv-antibodies

“…Then, a 2 nm titanium layer was coated, followed by a 48 nm of gold layer using K575X sputtering (Quorum Emitech, Kent, UK). 20,30,38 SPR chips were incubated with purified Fv-antibodies (10 μg mL −1 ) at 23 °C for 1 h. After washing with PBS, the BSA (1 mg mL −1 ) blocking was performed for 30 min. After washing with PBS again, the RBD antigen (1–500 nM) was flowed for 15 min (10 μL min −1 ) as the association step.…”

“…S2(B), ESI †). 18,[20][21][22]36,[38][39][40][41] Autodisplay of the Fv-antibody on the outer membrane of E. coli…”

Metal–organic frameworks (MOFs) as apt luminescent probes for the detection of biochemical analytes

“…87 Therefore, accurate imaging and sensing of glutathione in vivo is of great significance for understanding its related physiological or pathological activities, which is conducive for disease prevention or the development of new therapeutic techniques. At present, the main methods for detecting GSH in organisms are high performance liquid chromatography, 88 electrochemistry, 89 colorimetry, 90 photoelectric chemical method, 91 surface-enhanced Raman spectroscopy, 92 enzyme-linked immunosorbent, 93 and fluorescent assays. 94 Compared to other detection methods, fluorescence detection possesses non-destructive characteristics, real-time monitoring, rapid response, high selectivity and sensitivity, etc.…”