Regenerating dynamic organs using biomimetic patches

Chansoria, Parth; Etter, Emma L.; Nguyen, Juliane

doi:10.1016/j.tibtech.2021.07.001

Cited by 25 publications

(54 citation statements)

References 114 publications

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“…In the presence of a photoinitiator within the solution, the methacrylate moieties within and between neighboring polymeric chains can be covalently bound upon UV exposure, , leading to an irreversibly photo-cross-linked matrix. This stable matrix and its biocompatible and biodegradable properties offer a wide variety of applications such as scaffolds for tissue engineering and drug delivery, , wound healing, biological templates for studying disease progression, and extrusion and VAT photopolymerization bioprinting …”

Section: Introductionmentioning

confidence: 99%

Characterizing the Effects of Synergistic Thermal and Photo-Cross-Linking during Biofabrication on the Structural and Functional Properties of Gelatin Methacryloyl (GelMA) Hydrogels

Chansoria

Asif

Polkoff

et al. 2021

ACS Biomater. Sci. Eng.

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Gelatin methacryloyl (GelMA) hydrogels have emerged as promising and versatile biomaterial matrices with applications spanning drug delivery, disease modeling, and tissue engineering and regenerative medicine. GelMA exhibits reversible thermal cross-linking at temperatures below 37 °C due to the entanglement of constitutive polymeric chains, and subsequent ultraviolet (UV) photo-cross-linking can covalently bind neighboring chains to create irreversibly crosslinked hydrogels. However, how these cross-linking modalities interact and can be modulated during biofabrication to control the structural and functional characteristics of this versatile biomaterial is not well explored yet. Accordingly, this work characterizes the effects of synergistic thermal and photo-cross-linking as a function of GelMA solution temperature and UV photo-cross-linking duration during biofabrication on the hydrogels' stiffness, microstructure, proteolytic degradation, and responses of NIH 3T3 and human adiposederived stem cells (hASC). Smaller pore size, lower degradation rate, and increased stiffness are reported in hydrogels processed at lower temperature or prolonged UV exposure. In hydrogels with low stiffness, the cells were found to shear the matrix and cluster into microspheroids, while poor cell attachment was noted in high stiffness hydrogels. In hydrogels with moderate stiffness, ones processed at lower temperature demonstrated better shape fidelity and cell proliferation over time. Analysis of gene expression of hASC encapsulated within the hydrogels showed that, while the GelMA matrix assisted in maintenance of stem cell phenotype (CD44), a higher matrix stiffness resulted in higher pro-inflammatory marker (ICAM1) and markers for cell-matrix interaction (ITGA1 and ITGA10). Analysis of constructs with ultrasonically patterned hASC showed that hydrogels processed at higher temperature possessed lower structural fidelity but resulted in more cell elongation and greater anisotropy over time. These findings demonstrate the significant impact of GelMA material formulation and processing conditions on the structural and functional properties of the hydrogels. The understanding of these material-process-structure−function interactions is critical toward optimizing the functional properties of GelMA hydrogels for different targeted applications.

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Section: Introductionmentioning

confidence: 99%

Characterizing the Effects of Synergistic Thermal and Photo-Cross-Linking during Biofabrication on the Structural and Functional Properties of Gelatin Methacryloyl (GelMA) Hydrogels

Chansoria

Asif

Polkoff

et al. 2021

ACS Biomater. Sci. Eng.

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“…Indeed, cardiac tissue normally contracts both longitudinally and transversely during systole with an auxetic surface deformation having a Poisson's ratio between −0.8 and −0.6. 69 Therefore, auxetic films or patches can match the mechanical behavior of dynamic organs. In 2020, Olvera et al 201 fabricated PPy coated auxetic missing rib and square patches using PCL for the treatment of myocardial The results suggested that the number of attached cells was higher for the Aux-PPy patches compared to the square-PPy patches.…”

Section: Significance Of Auxetic Mechanical Metamaterials In Biomedic...mentioning

confidence: 99%

“…In organs, besides the intrinsic biomechanical anisotropy, meaning different properties through the directions due to the preferential orientation of the cells and ECM components, the morphology of the fibers creates interstitial voids (i.e., in cardiac fibers, smooth muscle fibers, and the collagen fibers of the skin and lung) . This morphology based on interstices produces volumetric deformation and simultaneous stretching and contraction in multiple directions at the organ surface that agree with the auxetic property.…”

Section: Significance Of Auxetic Mechanical Metamaterials In Biomedic...mentioning

confidence: 99%

“…68 For instance, auxetic films or patches can properly fit the requirements for dynamic organs that present change shape as part of their normal function (i.e., heart, lung, stomach, and intestines). 69 In this context, auxetic cardiac patches have been developed for treating myocardial infarction (MI) 70 owing to their multifunctional properties. In addition, these scaffolds could be applied for a wider range of biomedical engineering applications, such as bone screws, intervertebral discs, cartilage, and bone tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

“…In particular, for tissue engineering, the outstanding properties of auxetic polymer-based scaffolds with tunable Poisson’s ratio and adequate mechanical behavior were found to be useful in controlling the stress distribution throughout the scaffolds’ structure designed to interact with cells, resulting in enhanced cell proliferation, attachment, and migration. − A few studies have demonstrated the huge potential of auxetic mechanical metamaterials in the biomedical field, in particular, for tissue engineering to help regenerate human organs . For instance, auxetic films or patches can properly fit the requirements for dynamic organs that present change shape as part of their normal function (i.e., heart, lung, stomach, and intestines) . In this context, auxetic cardiac patches have been developed for treating myocardial infarction (MI) owing to their multifunctional properties.…”

Section: Introductionmentioning

confidence: 99%

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Review: Auxetic Polymer-Based Mechanical Metamaterials for Biomedical Applications

Veerabagu

Palza

Quero

2022

ACS Biomater. Sci. Eng.

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Over the last three decades but more particularly during the last 5 years, auxetic mechanical metamaterials constructed from precisely architected polymer-based materials have attracted considerable attention due to their fascinating mechanical properties. These materials present a negative Poisson's ratio and therefore unusual mechanical behavior, which has resulted in enhanced static modulus, energy adsorption, and shear resistance, as compared with the bulk properties of polymers. Novel advanced polymer processing and fabrication techniques, and in particular additive manufacturing, allow one to design complex and customizable polymer architectures that are particularly relevant to fabricate auxetic mechanical metamaterials. Although these metamaterials exhibit exotic mechanical properties with potential applications in several engineering fields, biomedical applications seem to be one of the most relevant with a growing number of articles published over recent years. As a result, special focus is needed to understand the potential of these structures and foster theoretical and experimental investigations on the potential benefits of the unusual mechanical properties of these materials on the way to high performance biomedical applications. The present Review provides up to date information on the recent progress of polymer-based auxetic mechanical metamaterials mainly fabricated using additive manufacturing methods with a special focus toward biomedical applications including tissue engineering as well as medical devices including stents and sensors.

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Rationally Designed Anisotropic and Auxetic Hydrogel Patches for Adaptation to Dynamic Organs

Chansoria

Blackwell

Etter

et al. 2022

Adv Funct Materials

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Current hydrogel or fabric patches for organ repair are generally not designed to conform to the complex mechanics of dynamic organs such as the lung or heart. This study presents a new, biocompatible and bilayered, hydrogelbased patch platform, consisting of a non-fouling top layer and a cell adhesive bottom layer, that caters to the anisotropic and auxetic characteristics of dynamic organs. Integrated computational and experimental studies are used to screen over 116 unique anisotropic-auxetic architectures to establish design rules and tailor the patches to a broad range of target organ dynamics. The patches are then validated in ex vivo and in vivo animal models, where the auxetic patches outperformed non-auxetic patches in conforming to the volumetric dilation-contraction of dynamic organs. To further expand the functionality of the auxetic patch platform, novel hole-filling auxetic patches are developed. These hole-filling patches composited with fibrin robustly reduce pulmonary air leakage in rats with surgically induced lung puncture. This is the first demonstration of a rational patch design framework that features both anisotropic and auxetic properties to cater to a wide range of organ dynamics. These studies pave the way for future clinical development of biomimetic patches.

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Regenerating dynamic organs using biomimetic patches

Cited by 25 publications

References 114 publications

Characterizing the Effects of Synergistic Thermal and Photo-Cross-Linking during Biofabrication on the Structural and Functional Properties of Gelatin Methacryloyl (GelMA) Hydrogels

Characterizing the Effects of Synergistic Thermal and Photo-Cross-Linking during Biofabrication on the Structural and Functional Properties of Gelatin Methacryloyl (GelMA) Hydrogels

Review: Auxetic Polymer-Based Mechanical Metamaterials for Biomedical Applications

Rationally Designed Anisotropic and Auxetic Hydrogel Patches for Adaptation to Dynamic Organs

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