Regeneration and bioengineering of the gastrointestinal tract: Current status and future perspectives

Orlando, Giuseppe; García‐Arrarás, José E.; Soker, Tom; Booth, Christopher; Sanders, Brian; Ross, Christina; Coppi, Paolo De; Farney, Alan C.; Rogers, Jeffrey; Stratta, Robert J.

doi:10.1016/j.dld.2012.04.005

Cited by 15 publications

(11 citation statements)

References 74 publications

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“…In last two decades translational research has progressed to make non modular tissue constructs such as skin, bladders, vessels and upper airways. 13 For their development, autologous cells are seeded on either artificial or natural supporting scaffolds. 13 These constructs are implanted without the reconstruction of the vascular supply, and the nutrients and oxygen as they receive supply from adjacent tissues by diffusion.…”

Section: Cell-printing Methodsmentioning

confidence: 99%

“…13 For their development, autologous cells are seeded on either artificial or natural supporting scaffolds. 13 These constructs are implanted without the reconstruction of the vascular supply, and the nutrients and oxygen as they receive supply from adjacent tissues by diffusion. Models of functioning hearts and livers have been engineered using "natural tissue" scaffolds and efforts are underway to produce kidneys, pancreas and small intestine.…”

Section: Cell-printing Methodsmentioning

confidence: 99%

“…More specifically, in organ bioengineering cells are seeded on biological or synthetic scaffolding materials ex vivo and allowed to either mature in bioreactors or be implanted without undergoing any maturation. 13 As the extracellular matrix is capable of driving complexity during development, hence greater attention must be paid to nanoscale cues to recapitulate complexity in engineered tissues. But mimicking the natural extracellular matrix is one of the critical and challenging technological barriers for scaffold engineering.…”

mentioning

confidence: 99%

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Role of Biological Scaffolds, Hydro Gels and Stem Cells in Tissue Regeneration Therapy

Upadhyay¹

2017

ATROA

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Present review article emphasizes role of biological scaffolds, hydrogels and stem cells in tissue engineering mainly in regeneration or repairing of damaged tissues. Highly porous scaffold biomaterials are developed which act as templates for tissue regeneration and potentially guide the growth of new tissue. Present article also describes de-cellularization, cell printing, vascularization, integration of cross linking of methods for scaffolding of biomaterials to reproduce and recapitulate complexity in engineered tissues. It also explains different scaffold types, polymer hydrogels which are necessary for formation of microstructure, cell attachment, differentiation, tissue vascularization and integration. It also explains use of induced pluripotent stem cells (iPSCs) and engineered MSCs as new diagnostic and potential therapeutic tools to remove sustained damage and complications from organ failures. These engineered MSCs assist in making self-assembling supramolecular hydrogels, which have larger applications in cell therapy of intractable diseases and tissue regeneration. No doubt development of more advanced biomaterials, growth factors and stem cell derived products/factors and tissue transplantation methods based on cell regeneration programming will revolutionize the clinical therapeutics. This article suggests identification of new biomaterials/biomolecules such as growth factors, scaffolds, integration, adhesion and regulatory molecules and cell secreted factors which can induce major metabolic and signaling pathways during phase of tissue repairing and induction of regeneration. This innovative research area needs many conceptual improvements in organ therapies, methods and technological advancement to scale more services to the human society.

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Section: Cell-printing Methodsmentioning

confidence: 99%

Section: Cell-printing Methodsmentioning

confidence: 99%

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confidence: 99%

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Role of Biological Scaffolds, Hydro Gels and Stem Cells in Tissue Regeneration Therapy

Upadhyay¹

2017

ATROA

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“…The need for new intestinal tissues arises from many disorders including intestinal ischemia, tumors, and infl ammatory bowel disease [2]. Bioengineering of the small intestine is becoming an attractive fi eld of research where all efforts and investments are undoubtedly justifi ed [1,[3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…The small bowel is possibly the only segment of the digestive tract that may be considered as absolutely essential to life [1]. The need for new intestinal tissues arises from many disorders including intestinal ischemia, tumors, and infl ammatory bowel disease [2].…”

Section: Introductionmentioning

confidence: 99%

Multiplication of the Enterocyte Mass by Serosal Patch Technique / Umnožavanje Enterocitne Mase Tehnikom Seroznog Patch-A

et al. 2015

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Damage to the small intestine and impairment of the intestinal epithelium occur in various diseases, resulting in a need for new epithelium. Therefore, bioengineering of the small intestine is becoming an attractive fi eld of research where all contributions are highly appreciated.The purpose of this study was to determine the possibility of the multiplication of the enterocyte mass using the technique of serosal patch with the application of hematopoietic stem cells, as well as the assessment of the quality of newly formed mucosa.Sixty Mill Hill hooded rats were divided in 4 groups, 15 animals each. In the control group animals, the patch was not created. In the other three groups, the animals were operated on and in each group 8 parietal and 7 visceral patches have been created. One of the groups with operated animals (Group NS) was not postoperatively treated. The second group of operated animals (Group G) was stimulated with granulocyte colony-stimulating factor (G-CSF). The third group of operated animals (Group GM) was stimulated with recombined humane granulocyte-macrophage colony-stimulating factor (rHuGM-CSF).In the group of animals that were not stimulated, epithelium proliferated slowly. In the group of animals stimulated with G-CSF stimulants, the epithelium initially proliferated rapidly, but appeared atrophic after eight weeks. Stimulation by rHuGM-CSF led to faster epithelization, and epithelium showed signs of advancing proliferation after eight weeks.We confi rmed the possibility of enterocyte mass multiplication by using the serosal patch technique, as well as that stimulation with rHuGM-CSF is more effective than stimulation with G-CSF.

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Mimicking Human Pathophysiology in Organ‐on‐Chip Devices

et al. 2018

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Convergence of life sciences, engineering, and basic sciences has opened new horizons for biologically inspired innovations, and a considerable number of organ‐on‐a‐chip platforms have been developed for mimicking physiological systems of biological organs such as the brain, heart, lung, kidney, liver, and gut. Various biophysicochemical factors can also be introduced into such organ‐on‐a‐chip platforms to study metabolic and systemic effects spanning from drug toxicity to different pathologic manifestations. There is also a pressing need to develop better disease models for common pathologies using variations of these platforms. This can be achieved by recapitulating the unique microenvironment of a disease to investigate the cause and development of abnormal conditions as well as the structural and functional changes resulting from such a pathology. In this review, the organ‐on‐a‐chip platforms that have been developed to model different pathologies of neurodegenerative, cardiovascular, respiratory, hepatic, and digestive systems, along with cancer are summarized. Although the field is still in its infancy, it is anticipated that developing disease model‐on‐a‐chip platforms will likely be a valuable addition to the field of disease modeling, pathology studies, and improved drug discovery.

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Regeneration and bioengineering of the gastrointestinal tract: Current status and future perspectives

Cited by 15 publications

References 74 publications

Role of Biological Scaffolds, Hydro Gels and Stem Cells in Tissue Regeneration Therapy

Role of Biological Scaffolds, Hydro Gels and Stem Cells in Tissue Regeneration Therapy

Multiplication of the Enterocyte Mass by Serosal Patch Technique / Umnožavanje Enterocitne Mase Tehnikom Seroznog Patch-A

Mimicking Human Pathophysiology in Organ‐on‐Chip Devices

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