Regeneration and myogenic cell proliferation correlate with taurine levels in dystrophin- and MyoD-Deficient muscles

McIntosh, Laura; Garrett, Kerryn L.; Megeney, Lynn A.; Rudnicki, Michael A.; Anderson, Judy E.

doi:10.1002/(sici)1097-0185(199810)252:2<311::aid-ar17>3.0.co;2-q

Cited by 54 publications

(56 citation statements)

References 44 publications

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“…2, A and B) for 34 min on each voxel. Peaks with contributions Cr/PCr [total Cr (tCr)], choline (Cho), Tau, and intraand extramyocellular lipids (IMCL and EMCL, respectively) were examined (45)(46)(47).…”

Section: Methodsmentioning

confidence: 99%

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Early metabolic changes measured by ¹H MRS in healthy and dystrophic muscle after injury

Pratt

Spangenburg

et al. 2012

Journal of Applied Physiology

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Xu S, Pratt SJ, Spangenburg EE, Lovering RM. Early metabolic changes measured by 1 H MRS in healthy and dystrophic muscle after injury. J Appl Physiol 113: 808 -816, 2012. First published June 28, 2012 doi:10.1152/japplphysiol.00530.2012.-Skeletal muscle injury is often assessed by clinical findings (history, pain, tenderness, strength loss), by imaging, or by invasive techniques. The purpose of this work was to determine if in vivo proton magnetic resonance spectroscopy ( 1 H MRS) could reveal metabolic changes in murine skeletal muscle after contraction-induced injury. We compared findings in the tibialis anterior muscle from both healthy wild-type (WT) muscles (C57BL/10 mice) and dystrophic (mdx mice) muscles (an animal model for human Duchenne muscular dystrophy) before and after contraction-induced injury. A mild in vivo eccentric injury protocol was used due to the high susceptibility of mdx muscles to injury. As expected, mdx mice sustained a greater loss of force (81%) after injury compared with WT (42%). In the uninjured muscles, choline (Cho) levels were 47% lower in the mdx muscles compared with WT muscles. In mdx mice, taurine levels decreased 17%, and Cho levels increased 25% in injured muscles compared with uninjured mdx muscles. Intramyocellular lipids and total muscle lipid levels increased significantly after injury but only in WT. The increase in lipid was confirmed using a permeable lipophilic fluorescence dye. In summary, loss of torque after injury was associated with alterations in muscle metabolite levels that may contribute to the overall injury response in mdx mice. These results show that it is possible to obtain meaningful in vivo 1 H MRS regarding skeletal muscle injury.in vivo magnetic resonance spectroscopy; eccentric injury; mdx; muscle damage INTENSE PHYSICAL EXERCISE can cause skeletal muscle damage and inflammation, resulting in pain and a loss of maximal force production. Skeletal muscle injury resulting from high-force lengthening contractions, such as intense physical exercise, is clinically termed a "muscle strain" and is one of the most common ailments seen by physicians. A diagnosis of acute muscle strains is still typically made based on physical exam and patient history. Several direct and indirect indicators of skeletal muscle damage have been observed, including disruption of contractile tissue, cellular accumulation of calcium, delayed onset muscle soreness, a prolonged reduction in muscular strength and range of motion, and an increase in the appearance of muscle proteins in the blood, such as creatine (Cr) kinase (CK), lactate dehydrogenase, and myoglobin (2, 6, 19). Duchenne muscular dystrophy (DMD) is characterized clinically by severe, progressive, and irreversible loss of muscular function. The disease is caused by the lack of dystrophin, a large membrane-associated protein expressed in skeletal muscle and localized to the inner face of the sarcolemma (63). There are significant parallels between the damage incurred after lengthening ("eccentric") contractions ...

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Section: Methodsmentioning

confidence: 99%

“…Ex vivo 1 H MRS work has shown lower levels of taurine (Tau) and Cr in resting mdx muscle but increases in Tau during an increase in muscle regeneration after crush injury (47). However, to the best of our knowledge, there are no high-resolution in vivo 1 H MRS studies examining spectroscopic features in injured mice.…”

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confidence: 99%

Early metabolic changes measured by ¹H MRS in healthy and dystrophic muscle after injury

Pratt

Spangenburg

et al. 2012

Journal of Applied Physiology

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“…In both tissue types from the mdx mouse, taurine was elevated compared with the levels in control tissue samples. This metabolite has previously been reported to be a biomarker for dystrophic tissue in skeletal muscle 66,67 and is thought to be an adaptive response to a loss of dystrophin. 63 Duchenne muscular dystrophy has also been investigated in intact cardiac tissue using high resolution magic angle spinning (MAS) NMR, coupled with PR.…”

Section: Metabonomic Studies Of Physiological Variationmentioning

confidence: 97%

NMR-based metabonomic approaches for evaluating physiological influences on biofluid composition

et al. 2004

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Strategies such as genomics, proteomics and metabonomics are being applied with increasing frequency in the pharmaceutical industry. For each of these approaches, toxicological response can be measured by terms of deviation from control or baseline status. However, in order to accurately define drug-induced response, it is necessary to characterize the normal degree of physiological variation in the absence of stimuli. Here, 1 H NMR spectroscopic-based analyses of the metabolic composition of urine in experimental animals under various normal physiological conditions are reviewed. In particular, the effects of inter-animal and diurnal variation, gender, age, diet, species, strain, hormonal status and stress on the biochemical composition of urine are explored. Pattern recognition methods facilitate the comparison of urine NMR spectra over a given time-course, enabling the establishment of changes in profile and highlighting the dynamic metabolic status of an organism. Thus metabonomic approaches based on information-rich spectroscopic data sets can be used to evaluate normal physiological variation and for investigation of drug safety issues.

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“…These strategies have brought stem cell therapy to the forefront of therapeutic muscle regeneration. The anticipation of cell therapy strategies has also encouraged study of non-invasive technologies such as magnetic resonance spectroscopy and imaging to monitor the success of cell engraftment or treatment (Cahill et al, 2004;Hoehn et al, 2002;McIntosh et al, 1998a;McIntosh et al, 1998b;McIntosh et al, 1998c). Again, of particular importance for therapies involving satellite cells as conveyors of muscle plasticity is the establishment of new satellite cell precursors (through some influences that induce quiescence) on the newly transformed host fibres.…”

Section: (2) Satellite Cells As a Conveyance In Skeletal Muscle Plastmentioning

confidence: 99%

The satellite cell as a companion in skeletal muscle plasticity:currency, conveyance, clue, connector and colander

Anderson

2006

Journal of Experimental Biology

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THE JOURNAL OF EXPERIMENTAL BIOLOGY 2277 Satellite cells in muscle plasticity formed cells fusing to form myoblasts and finally multinucleated muscle fibres.'That conference was a landmark in studies of the satellite cell and muscle regeneration, and was attended by many of the notable and pioneering contributors to the field of muscle development and muscle regeneration. Muir summarized the debate on a working definition of the satellite cell, as follows (Muir, 1970):'The satellite cell of striated muscle can be defined as a mononucleated cell, whose cytoplasm does not contain myofilaments, and which is enclosed by or lies within the basement membrane component of the sarcolemma of the striated muscle fibre. This definition does not exclude cells which are partially or completely separated from the muscle fibre plasma membrane by extensions of the basement membrane, providing that the basement membrane forms a complete investment of their outer surfaces. ' Although there were presentations detailing the observed uptake of 3 H-thymidine into nuclei of satellite cells (Hay, 1970), the role of satellite cells as precursors was not fully established in 1969. In fact there was still very strong debate that a muscle fibre could fragment into blastema cells that led to new formation of muscle. Even at this time, there was more than one paper on the appearance of osteogenic and chondrogenic tissues from minces of muscle replaced under the skin, and a report of muscle fibre nuclei contributed by a labelled connective tissue grafted into a salamander limb during regeneration (Steen, 1970).It wasn't until two seminal reports from the laboratory of Dr Charles Leblond at McGill University (Moss and Leblond, 1970;Moss and Leblond, 1971) that skeletal muscle satellite cells were convincingly identified as the source of precursor cells that proliferate and fuse to form new skeletal muscle fibres. Time-course studies of labelled nuclei in growing muscle showed that satellite cells were the only muscle cells that had incorporated 3 H-thymidine. The report followed work on colchicine-treated rats (MacConnachie et al., 1964) that showed mitotic figures only in the peripheral 'satellite' position on muscle fibres. The idea that muscle fibre nuclei give rise to the increasing number of myonuclei during the growth was convincingly ruled out (Enesco and Puddy, 1964). The notion that satellite cells contribute these domains to a growing or regenerating fibre, one at a time, is daunting in light of the expenditure of proliferative energy and fusion events. However, it speaks strongly to one of the major roles of satellite cells as a currency of muscle (see below). Satellite cells were also observed on intrafusal fibres (Katz, 1961). Two types of satellite cells were identified on these socalled muscle spindle fibres and were distinguished from those on extrafusal fibres (Maynard and Cooper, 1973), although at least one would now be called a myoblast.Although typically quiescent in normal adult muscle, satellite cells are generally con...

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Regeneration and myogenic cell proliferation correlate with taurine levels in dystrophin- and MyoD-Deficient muscles

Cited by 54 publications

References 44 publications

Early metabolic changes measured by ¹H MRS in healthy and dystrophic muscle after injury

Early metabolic changes measured by ¹H MRS in healthy and dystrophic muscle after injury

NMR-based metabonomic approaches for evaluating physiological influences on biofluid composition

The satellite cell as a companion in skeletal muscle plasticity:currency, conveyance, clue, connector and colander

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Regeneration and myogenic cell proliferation correlate with taurine levels in dystrophin- and MyoD-Deficient muscles

Cited by 54 publications

References 44 publications

Early metabolic changes measured by 1H MRS in healthy and dystrophic muscle after injury

Early metabolic changes measured by 1H MRS in healthy and dystrophic muscle after injury

NMR-based metabonomic approaches for evaluating physiological influences on biofluid composition

The satellite cell as a companion in skeletal muscle plasticity:currency, conveyance, clue, connector and colander

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Early metabolic changes measured by ¹H MRS in healthy and dystrophic muscle after injury

Early metabolic changes measured by ¹H MRS in healthy and dystrophic muscle after injury