Regeneration of Aegle marmelos (l.) Through Enhanced Axillary Branching from Cotyledenory Node

Abstract

A complete protocol is standardized for invitro micropropogation of Aegle marmelos for the first time using cotyledonary nodes derived through invitro raised seedlings.Higher percentage of direct multiple shoots were regenerated from cotyledonary nodal segments through forced axillary branching. The cotyledonary nodes of invitro raised seedlings were used as explants for shoot formation on MS Medium supplemented with Cytokinins (BAP) and Auxins (NAA), either alone or in combinations. Maximum (80%) shoots having shoot length of 2-3 cm were achieved on MS medium fortified with BAP (1.0 mg/l) and NAA (0.5mg/l).By repeating sub culturing of the cotyledonary node on shoot multiplication medium followed by shoot elongation medium after each harvest of the newly formed shoots. Thus, from a single cotyledonary node, about 20-25 shoots were obtained. Shoots formed in vitro were best rooted on MS medium supplemented with 1.0-2.0 mg/l Napthalene acetic acid(NAA),