The present investigation was conducted to observe the effect of various concentrations (0.1 to 1.5 gm/100ml) of micronutrients (Zn, Mn, B, Cu, Mo, Fe) with yeast extract mannitol media on agriculturally important Rhizobium species isolated from root nodules of Cajanus cajan and to determine the effect of multi-micronutrient yeast extract mannitol media prepared of maximum growth concentrations of micronutrients on rhizobial growth. Concentrations of Zn (0.1%), Mn(0.3%), B(0.1%), and Mo(0.1%) with yeast extract mannitol media composition represented increased number of CFU against control. The growth of Rhizobium in multimicronutrient yeast extract mannitol media decreased by 25% compared to control.

The present study was undertaken to evaluate the most suitable and efficient media of low concentration growth regulators for shoot induction and subsequent multiplication in Centella asiatica L. an important and valuable medicinal plant. The best shoot induction (82±2.2%) was found in MS medium supplemented with 0.5 mg/ l BAP + 0.1 mg/ l NAA from axillary meristem explant. Combination of 0.5 mg/ l BAP + 0.5 mg/ l NAA and 0.5 mg/ l BAP + 0.5 mg/ l KN gave the most effective shoot multiplication from initiated meristem cultures. The elongated shoots were rooted in MS medium supplemented with different concentrations of auxins (NAA and IBA). Lower concentration of IBA was more suitable for root induction when compared to NAA. The success of plant tissue culture for in vitro culture of Centella asiatica was encouraged by acclimatization of the plantlets in the field conditions. About 95% plantlets survived under field conditions.

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A complete protocol is standardized for invitro micropropogation of Aegle marmelos for the first time using cotyledonary nodes derived through invitro raised seedlings.Higher percentage of direct multiple shoots were regenerated from cotyledonary nodal segments through forced axillary branching. The cotyledonary nodes of invitro raised seedlings were used as explants for shoot formation on MS Medium supplemented with Cytokinins (BAP) and Auxins (NAA), either alone or in combinations. Maximum (80%) shoots having shoot length of 2-3 cm were achieved on MS medium fortified with BAP (1.0 mg/l) and NAA (0.5mg/l).By repeating sub culturing of the cotyledonary node on shoot multiplication medium followed by shoot elongation medium after each harvest of the newly formed shoots. Thus, from a single cotyledonary node, about 20-25 shoots were obtained. Shoots formed in vitro were best rooted on MS medium supplemented with 1.0-2.0 mg/l Napthalene acetic acid(NAA),

Aegle marmelos, a popular medicinal plant and is used in traditional medicine to treat numerous ailments. Aegle marmelos are reported to have a wide spectrum of pharmacological properties. Its medicinal usage is well-documented in in different traditional medicine such as the Ayurveda, Unani, Siddha, and other conventional medical systems.