Regeneration of Bladder Urothelium, Smooth Muscle, Blood Vessels and Nerves Into an Acellular Tissue Matrix

Sutherland, Ronald S.; Baskin, Laurence S.; Hayward, Simon W.; Cunha, Gerald R.

doi:10.1097/00005392-199608001-00002

Cited by 95 publications

(124 citation statements)

References 21 publications

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“…These properties together with its biocompatibility make ECM an ideal biomaterial for tissue engineering purposes. ECM harvested from the small intestine, skin, liver, pancreas, and urinary bladder has been successfully used to promote the regeneration of tissues and organs in both animal models and human (14)(15)(16)(17)(18). Several types of mono-or multilayer vascular grafts containing collagen and elastin have been obtained using different techniques (9,(19)(20)(21)(22)(23).…”

Section: Introductionmentioning

confidence: 99%

ECM-based triple layered scaffolds for vascular tissue engineering

Grandi¹

2011

Int J Mol Med

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Abstract. The present study focused on the development of three layered small-diameter (<6 mm) extracellular matrix (ECM)-based vessels. These were engineered artificially through the freeze-drying technique. A layer of decellularized bovine aorta (DAM) was deposited on a mandrel and, after lyophilization, it was dipped into a poly-L-lactide acid (PLLA)/polyethylene glycol (PEG) 2000 dichloromethane solution then quickly wrapped with a pre-prepared thin DAM sheet. Mechanical properties of three-layered scaffolds were evaluated by means of uniaxial tensile measurement. Furthermore, human endothelial and smooth muscle cells were seeded on internal and external scaffold surfaces, respectively, and co-cultured for 7 days. Our results demonstrate that i) ECM components provide suitable stimuli for cell adhesion and proliferation, ii) the microporous intermediate PLLA/ PEG2000 layer is responsible for the scaffold resistance and iii) the layered deposition technique can be considered a valuable method to obtain layered vascular scaffolds of different sizes and with a good compromise between stiffness and elasticity for optimal cell organization.

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Section: Introductionmentioning

confidence: 99%

ECM-based triple layered scaffolds for vascular tissue engineering

Grandi¹

2011

Int J Mol Med

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“…The integration of these matrices into the host tissue may benefit from a microvascular network capable of anastomosing with the host microvasculature, thereby ensuring an adequate supply of blood and nutrients to the implants. In the last few years, acellular matrices have been successfully used to substitute and repair skin, bladder, urethra, small bowel, and skeletal muscle defects (Sutherland et al, 1996;Takami et al, 1996;Parnigotto et al, 2000a, b;Marzaro et al, 2002). Iurlaro et al (2002) described a new method for the neovascularization of polyurethane meshes from explants of rat aorta.…”

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confidence: 99%

Angiogenic response induced by acellular aortic matrix in vivo

et al. 2004

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In this study, we investigated the angiogenic response induced by acellular aortic matrices implanted in vivo onto the chick embryo chorioallantoic membrane (CAM), a useful model for such investigation. Results showed that acellular matrices were able to induce a strong angiogenic response comparable to that of fibroblast growth factor 2 (FGF-2), a wellknown angiogenic cytokine. The angiogenic response was further increased when exogenous FGF-2 or transforming growth factor beta 1 (TGF-␤1) were added to the matrices and inhibited by the addition of an anti-FGF-2 or anti-TGF-␤1 antibodies. The response may be considered dependent on a direct angiogenic effect exerted by the matrices and in part also by the presence of FGF-2 and TGF-␤1 in the acellular matrices.

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“…The absence of chronic cystitis in this study, which is one of the most leading causes of failure of bladder augmentation, can be explained in the light of good regeneration of the urothelium that started at the adjacent edges of the native bladder [33], subsequently rested on the basement membrane after completely covering the graft and gradually developed into a urothelium resembling a normal bladder epithelium [15]. This newly formed urothelium maintained a tight permeability barrier, and damage to it predisposes it to leakage of urine components into the underlying bladder layers, leading to symptoms of cystitis [22] or absorption of urine with subsequent alteration of urea and creatinine levels.…”

Section: Discussionmentioning

confidence: 54%

Full-Thickness Hysterocystoplasty for Management of a Large Bladder Defect: Experimental Study in Goats

Abdelrhman

Seddek

Bakr

et al. 2013

The Journal of Veterinary Medical Science

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ABSTRAcT. The uterine tissue of three healthy nonpregnant goats was evaluated experimentally as a graft for closure of a bladder defect. Goats were subjected to ovariectomy, and then one detubularized uterine horn was used as a graft to close a large defect in the ventral aspect of the bladder. Follow up included monthly radiographic and ultrasonographic examinations and evaluations of kidney function and electrolytes changes for six months. The goats were euthanized after six months, and both the bladder and the graft were examined macroscopically and histologically. The technique required less invasive procedures than those described with alternative techniques of cystoplasty, achieved a high survival rate without life-threatening complications post surgery, was associated with no significant changes in kidney function and electrolytes levels, showed proper healing of the transplanted graft by regeneration rather than repair without scaring or fibrosis and with complete covering of the graft by a healthy urothelium and was associated with no malignant transformation. Hysterocystoplasty is a noninvasive, non-life-threatening technique due to the close position of the bladder to the uterus, and proper healing of the graft reflects absence of pressure on its blood supply. It is an acceptable alternative technique for closure of a large bladder defect and avoids disadvantages of alternative techniques of cystoplasty. It might be accepted by owners of pets, and further studies in clinical cases of dogs are advised. Although cystoplasty is a historic topic that has been discussed well in scientific literature and argued at legendary proportion among urologists, the search for an ideal organ to replace a diseased bladder remains elusive for reconstructive urologists [32]. Many augmentation cystoplasty or neobladder techniques have been used routinely for treatment of reduced bladder compliance and capacity secondary to bladder infection and inflammation [27], neurogenic dysfunction or congenital bladder disorders [16], detrusor over activity [10], large bladder defects [8,9], bladder necrosis [30] and bladder tumors [7,12]. Although encouraging animal and human results have been reported with different techniques, each technique had its own limitations, complications and disadvantages [2,4,11,27].The ileum and colon have been used for decades for reconstruction of the bladder, in spite of the harmful contact of urine with the mucosa of the augmenting patch [23], which is predisposed to multiple short-and/or long-term complications like chronic bacteriuria or mucous secretion [1,23], stone formation [1,11,29], disturbance of calciumphosphate metabolism with potential bone growth retardation [24,28], altered hepatic and electrolyte metabolism [24], gastrointestinal motility disorders [23], augmenting patch perforation [1,6] and malignant transformation of the augmenting patch [1,14].Gastrocystoplasty has been described as an alternative technique; unfortunately, as the popularity of gastrocystoplasty increased, so did ...

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Regeneration of Bladder Urothelium, Smooth Muscle, Blood Vessels and Nerves Into an Acellular Tissue Matrix

Cited by 95 publications

References 21 publications

ECM-based triple layered scaffolds for vascular tissue engineering

ECM-based triple layered scaffolds for vascular tissue engineering

Angiogenic response induced by acellular aortic matrix in vivo

Full-Thickness Hysterocystoplasty for Management of a Large Bladder Defect: Experimental Study in Goats

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