Regeneration of grape (Vitis labruscana cv. Kyoho) by shoot-tip culture

Park, Hye-Jeong; Lee, Ho-Rim; Pyee, Jaeho; Cha, Hyeon-Cheol

doi:10.1007/bf03030350

Cited by 9 publications

(4 citation statements)

References 18 publications

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“…Nevertheless, there was no clear relationship between the concentration of BAP and the callus intensity. This could indicate that this response might be influenced by other factors (e.g., physiological state or nodal stem localization) related to the endogenous level of the plant growth regulators (PGRs) in the plant tissue [61,62].…”

Section: Induction Of Axillary Bud Developmentmentioning

confidence: 99%

In Vitro Propagation of an Endangered Helianthus verticillatus by Axillary Bud Proliferation

Nowakowska

Pavlović

Nowicki

et al. 2020

Plants

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Helianthus verticillatus (Asteraceae), whorled sunflower, is a perennial species restricted to a few locations in the Southeastern United States. Habitat loss has caused H. verticillatus to become rare, and since 2014, it has been federally listed as an endangered species. As a part of the recovery plan for the restoration and protection of H. verticillatus, an efficient micropropagation protocol based on axillary shoot proliferation was developed. Various concentrations of 6-benzylaminopurine (BAP; 0 to 4.44 µM) were examined for their morphogenetic potential in the regeneration of six genotypes of H. verticillatus from the nodal explants derived from greenhouse-grown plants. Both the BAP concentration and genotype had significant effects on the regeneration capacity of H. verticillatus. Although the induced buds were observed on ½-strength Murashige and Skoog medium without plant growth regulators, a higher rate of induction and bud development were achieved on media with either 0.88 or 2.22 µM BAP, regardless of the genotype. Successful rooting of the induced shoots was achieved within four weeks after the transfer from the induction medium to the fresh ½-strength MS medium, but the rooting efficiency was dependent on the plant’s genetic background. Regenerated plantlets, with well-developed shoots and roots, were acclimatized successfully to greenhouse conditions with a 97% survival rate. Simple sequence repeats (SSRs) markers were employed to assess the genetic uniformity of the micropropagated plants of H. verticillatus. No extraneous bands were detected between regenerants and their respective donor plants, confirming the genetic fidelity and stability of regenerated plants. To our knowledge, the protocol developed in this study is the first such report for this endangered species.

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Section: Induction Of Axillary Bud Developmentmentioning

confidence: 99%

In Vitro Propagation of an Endangered Helianthus verticillatus by Axillary Bud Proliferation

Nowakowska

Pavlović

Nowicki

et al. 2020

Plants

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“…The combination of various growth regulators and their concentrations significantly influences shoot length due to their effect on cell division and cell expansion (Gordon and Letham, 1975). Therefore, growth regulators are noticeably important for in vitro shoot proliferation, but some internal factors and nutrient conditions can modify their activities (Park et al, 2001). Shoot regeneration also depends upon endogenous levels of growth regulators in the explant and the position of node, as different types of buds showed different regeneration efficiencies in grape (Nontaswatsri et al, 2002).…”

Section: Growth Of In Vitro Shoot Culturesmentioning

confidence: 99%

Optimizing the concentrations of plant growth regulators for in vitro shoot cultures, callus induction and shoot regeneration from calluses of grapes

Khan¹,

Ahmed

Hafiz

et al. 2015

OENO One

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Aim: To optimize the concentrations of growth regulators in the media for the proficient micropropagation of grapevine (Vitis vinifera L.) cv. King’s Ruby.Methods and results: Apical meristems of the grape cultivar were used to establish in vitro shoot cultures. Nodal explants, each containing an axillary bud, taken from in vitro grown shoots were inoculated in shoot proliferation medium, i.e., half strength Murashige and Skoog (MS) medium supplemented with benzyl aminopurine (BAP), kinetin, glycine and gibberellic acid (GA3). A higher number of shoots (5.33) with greater shoot length (2.75 cm) was produced in the medium supplemented with 1.0 mg L-1 BAP and 0.1 mg L-1 GA3. Calluses were induced from leaf explants taken from in vitro grown shoots. Callus induction was greater (73.00%) on the medium containing 2.0 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D), 0.3 mg L-1 BAP and 0.2 mg L-1 α-naphthaleneacetic acid (NAA). The maximum frequency of shoot regeneration (53.33%) was achieved on the medium supplemented with 1.5 mg L-1 BAP and 0.5 mg L-1 NAA, and the regenerated shoots successfully formed roots on growth regulator-free half strength MS medium.Conclusion: Optimizing the concentration of BAP and GA3 and omitting the glycine and kinetin in the culture medium increased the number and length of shoots. Similarly, for inducing the callus of the leaf explants, taken from in vitro grown shoots, it is recommended to adjust the medium with the higher concentration of 2,4-D and lower concentrations of BAP. Moreover, the maximum number of shoots was regenerated on a medium supplemented with relatively high levels of both BAP and NAA (1.5 and 0.5 mg L-1, respectively). Finally, we suggest the half strength MS medium that is free from growth regulators for the root formation of the regenerated shoots.Significance and impact of the study: Optimizing the concentration of growth regulators is crucial for the efficient micropropagation of a grape cultivar. Knowing the specific balance between the growth regulators is necessary to establish in vitro shoot cultures, callus induction and shoot regeneration and, hence, to propagate disease-free true to type grape cultivars in a short time.

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“…Many studies have reported that rootstock varieties reveal higher organogenesis and somatic embryogenesis potential than hybrids and varieties belonging to the V. vinifera species [133]. However, several other factors were reported to influence the efficiency of grapevine in vitro plant regeneration, such as culture medium composition, especially the type and concentrations of plant growth regulators (PGRs) [134,135]; explants' developmental stage [136] and phyllotactic position [137]; light regime [138]; pH value [139], etc. Therefore, over the years, numerous studies were focused mainly on optimizing protocols for efficient regeneration across different grapevine genotypes.…”

Section: Grapevine Plant Regeneration Methodsmentioning

confidence: 99%

Biotechnologies and Strategies for Grapevine Improvement

Butiuc-Keul

Coste

2023

Horticulturae

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Grapevine (Vitis vinifera subsp. Vinifera) is one of the most widespread and economically important perennial fruit crops in the world. Viticulture has changed over the years in response to changing environmental conditions and market demands, triggering the development of new and improved varieties to ensure the crop’s sustainability. The aim of this review is to provide a perspective on the recent developments in biotechnology and molecular biology and to establish the potential of these technologies for the genetic improvement of grapevine. The following aspects are discussed: (i) the importance of molecular marker-based methods for proper cultivar identification and how NGS-based high-throughput technologies have greatly benefited the development of genotyping techniques, trait mapping, and genomic selection; (ii) the recent advances in grapevine regeneration, genetic transformation, and genome editing, such as new breeding technology approaches for enhanced grapevine yield, quality improvement, and the selection of valuable varieties and cultivars. The specific problems and challenges linked to grapevine biotechnology, along with the importance of integrating classical and new technologies, are highlighted.

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Regeneration of grape (Vitis labruscana cv. Kyoho) by shoot-tip culture

Cited by 9 publications

References 18 publications

In Vitro Propagation of an Endangered Helianthus verticillatus by Axillary Bud Proliferation

In Vitro Propagation of an Endangered Helianthus verticillatus by Axillary Bud Proliferation

Optimizing the concentrations of plant growth regulators for in vitro shoot cultures, callus induction and shoot regeneration from calluses of grapes

Biotechnologies and Strategies for Grapevine Improvement

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