Regeneration of hair and other skin appendages: A microenvironment‐centric view

Huang, Chenyu; Du, Yanan; Nabzdyk, Christoph S.; Ogawa, Rei; Koyama, Tadanobu; Orgill, Dennis P.; Fu, Xiaobing

doi:10.1111/wrr.12451

Cited by 14 publications

(9 citation statements)

References 93 publications

(170 reference statements)

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“…36 Although the mechanism by which HFSCs promote wound healing remains unclear, evidence suggests that pathways including the Wnt, Bmp, Notch and Sonic hedgehog (SHH) pathways are involved in hair follicle regeneration and wound repair, among which the Wnt/β-catenin pathway plays an important regulatory role. 37,38 Regarding the application of autologous growth factors, platelet-rich plasma (PRP) may be regarded as the representative which contains at least six major growth factors, including bFGF, PDGF, VEGF, EGF, TGF-β and IGF-1. 39 PRP can provide growth factors necessary for wound repair and regulate the dynamic balance between cells and extracellular matrix in the local microenvironment, providing favorable conditions for wound repair.…”

Section: Discussionmentioning

confidence: 99%

<p>Umbilical Cord-Derived Mesenchymal Stem Cell-Derived Exosomes Combined Pluronic F127 Hydrogel Promote Chronic Diabetic Wound Healing and Complete Skin Regeneration</p>

Yang¹,

Chen²,

Pan³

et al. 2020

IJN

340

213

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Chronic refractory wounds are a multifactorial comorbidity of diabetes mellitus with the characteristic of impaired vascular networks. Currently, there is a lack of effective treatments for such wounds. Various types of mesenchymal stem cell-derived exosomes (MSC-exos) have been shown to exert multiple therapeutic effects on skin regeneration. We aimed to determine whether a constructed combination of human umbilical cord MSC (hUCMSC)-derived exosomes (hUCMSC-exos) and Pluronic F-127 (PF-127) hydrogel could improve wound healing. Materials and Methods: We topically applied human umbilical cord-derived MSC (hUCMSC)-derived exosomes (hUCMSC-exos) encapsulated in a thermosensitive PF-127 hydrogel to a full-thickness cutaneous wound in a streptozotocin-induced diabetic rat model. The material properties and wound healing ability of the hydrogel and cellular responses were analyzed. Results: Compared with hUCMSC-exos, PF-127-only or control treatment, the combination of PF-127 and hUCMSC-exos resulted in a significantly accelerated wound closure rate, increased expression of CD31 and Ki67, enhanced regeneration of granulation tissue and upregulated expression of vascular endothelial growth factor (VEGF) and factor transforming growth factor beta-1 (TGFβ-1). Conclusion: The efficient delivery of hUCMSC-exos in PF-127 gel and improved exosome ability could promote diabetic wound healing. Thus, this biomaterial-based exosome therapy may represent a new therapeutic approach for cutaneous regeneration of chronic wounds.

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Section: Discussionmentioning

confidence: 99%

<p>Umbilical Cord-Derived Mesenchymal Stem Cell-Derived Exosomes Combined Pluronic F127 Hydrogel Promote Chronic Diabetic Wound Healing and Complete Skin Regeneration</p>

Yang¹,

Chen²,

Pan³

et al. 2020

IJN

340

213

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“…Si‐containing biomaterials are capable of altering the microenvironment to generate the biological effects . The microenvironment can largely influence various cellular aspects, such as biological characteristics, gene expression, and protein synthesis . Therefore, “ion therapy” was proposed as a novel therapeutic strategy for certain intractable diseases, which includes treatment with bioactive ions derived from silicate biomaterials …”

Section: Discussionmentioning

confidence: 99%

Regenerative and protective effects of calcium silicate on senescent fibroblasts induced by high glucose

Bian

et al. 2020

Wound Repair Regeneration

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Diabetic wounds are a common complication of diabetes and therefore a pressing issue for clinicians. High-glucose (HG)-induced fibroblast senescence is mainly responsible for delayed wound healing. Calcium silicate (CS), a kind of bioceramic, is thought to have regenerative properties. The aim of this study was to determine the regenerative and protective effects of CS on senescent fibroblasts induced by HG. Fibroblasts were passaged five times and treated with HG and CS. Compared with the normal glucose (NG) group, the proliferation, migration, and differentiation capacity of HG-induced fibroblasts significantly decreased (P < .05). After treatment with CS, the functions of HG-induced senescent fibroblasts were partly restored (P < .05). The mechanism of the regenerative and protective effects of CS may be related to the decreased reactive oxygen species generation, improved senescent state (SA-β-gal expression decreased), up-regulated expression of Smad2 and phosphorylated Smad2, and down-regulated expression of p16, p21, and p53. An in vivo experiment also demonstrated that CS had a therapeutic effect on diabetic wounds via differentiation of fibroblasts into myofibroblasts and enhanced collagen deposition. These results indicate that CS may be a promising candidate for diabetic wound therapy.

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“…The microenvironment of the host also exercises a great influence on the reconstruction of hair follicles [25]. The microenvironment typically consists of the following sections: cells (such as white blood cells, fibroblasts, and adipocytes), growth factors (such as transforming growth factor, vascular endothelial growth factor, and fibroblast growth factor), the extracellular matrix (laminin, fibronectin, and proteoglycan), and signaling molecules (WNT, bone morphogenetic protein, and Notch) [26–28].…”

Section: Discussionmentioning

confidence: 99%

Unlocking the vital role of host cells in hair follicle reconstruction by semi-permeable capsules

Fan

Miao

et al. 2017

PLoS ONE

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Organ regeneration is becoming a promising choice for many patients; however, many details about the mechanisms underlying organ regeneration remain unknown. As regenerative organs, hair follicles offer a good model to study the mechanisms associated with regenerative medicine. The relevant studies have mainly focused on donor cells, and there are no systematic studies involving the effect of host factors on hair follicle reconstruction. Thus, we intend to explore the effect of host cells on hair follicle reconstruction. Epidermal and dermal cells from red fluorescent protein (RFP) transgenic newborn mice were injected into green fluorescent protein (GFP) transgenic mice. In addition, we wrapped the mixed dermal and epidermal cells from GFP transgenic and RFP transgenic mice by the Cell-in-a-Box kit to form "capsules," so that the cells within would be isolated from host cells. These capsules were cultured in vitro and transplanted in vivo. Fully developed reconstructed hair follicles were observed after the injection of mixed cells. These reconstructed follicles mainly consisted of donor cells, as well as a small number of host cells. The encapsulated cells gradually aggregated into cell spheres in vitro without apparent differentiation towards hair follicles. With respect to the transplanted capsules, concentric circle structures were observed, but no hair follicles or hair shafts formed. When the concentric circle structures were transplanted in vivo, mature hair follicles were observed 30 days later. Host cells were found in the reconstructed hair follicles. Thus, we conclude that host cells participate in the process of hair follicle reconstruction, and they play a vital role in the process, especially for the maturation of reconstructed hair follicles. Furthermore, we established a special hair follicle reconstruction system with the help of capsules: transplant cells were isolated from host, but other factors from host could exchange with cells inside.

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Regeneration of hair and other skin appendages: A microenvironment‐centric view

Cited by 14 publications

References 93 publications

<p>Umbilical Cord-Derived Mesenchymal Stem Cell-Derived Exosomes Combined Pluronic F127 Hydrogel Promote Chronic Diabetic Wound Healing and Complete Skin Regeneration</p>

<p>Umbilical Cord-Derived Mesenchymal Stem Cell-Derived Exosomes Combined Pluronic F127 Hydrogel Promote Chronic Diabetic Wound Healing and Complete Skin Regeneration</p>

Regenerative and protective effects of calcium silicate on senescent fibroblasts induced by high glucose

Unlocking the vital role of host cells in hair follicle reconstruction by semi-permeable capsules

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