Regeneration of islet β‐cells in tree shrews and rats

Zhao, Yingjie; Liu, Yaqian; Yuan, Jingjing; Dai, Xin; Niu, Miaomiao; Sun, Xiaomei; Kuang, Dexuan; Wang, Wenguang; Tong, Pinfen; Li, Na; Xiang, Lei; Jia, Yongyi; Dai, Jiejie; Chen, Hua

doi:10.1002/ame2.12023

Cited by 4 publications

(2 citation statements)

References 37 publications

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“…This route of insulin secretion is impaired by reduced levels of adenosine triphosphate (ATP). This occurs because STZ interferes with the expression of GLUT2 and the citric acid cycle, reducing the production of nicotinamide adenine dinucleotide (NAD) and ATP below physiological levels (Zhao et al 2018). Thus, oxygen consumption in mitochondria decreases substantially, resulting in the formation of superoxide anions, and increased activity of the enzyme xanthine oxidase (Turk et al 1993).…”

Section: Resultsmentioning

confidence: 99%

Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic rats

et al. 2020

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The study aims to analyse the treatment of whey protein enriched with Stevia rebaudiana fraction in insulin secretion and its role mitigating streptozotocin-induced hyperglycemia in rats. Thus, diabetic animals were treated with whey protein enriched with S. rebaudiana fraction or with only the protein isolate or only the Stevia fraction. Insulin level in plasma was measured by radioimmunoassay and the viability of b cells was detected by immunohistochemistry. The results showed that diabetic animals treated with whey protein enriched with S. rebaudiana fraction had a greater recovery from insulinemia, with plasma levels similar to non-diabetic animals (* 0.13 ng/ mL). In addition, the same group showed a higher number of insulin-positive pancreatic B cells (* 66%) in immunohistochemistry analysis, while the diabetic groups treated with only the fraction of stevia or whey protein showed 38 and 59% of positive cells, respectively. These results show that the treatment may have restored the viability of streptozotocin-injured pancreatic B cells, and consequently increased insulin secretion, suggesting whey protein enriched with S. rebaudiana fraction can be used an adjunct/supplement in diabetic treatment.

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Section: Resultsmentioning

confidence: 99%

Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic rats

et al. 2020

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“…However, in this experiment, the severe DM model induced DKA even though in the exogenous insulin treatment, making intra-islet regeneration of beta cells unlikely. Additionally, although alpha-to-beta cell conversion is considered possible, it was not observed histopathologically over a 21-day period in a high-dose STZ model (60 mg/kg) in rats, instead, this research team histopathologically confirmed that regeneration primarily occurred from centroacinar exocrine cells [ 20 ]. However, from a functional perspective, BGLs remained high after STZ administration, and there was no significant increase in blood insulin levels.…”

Section: Discussionmentioning

confidence: 99%

Induction of Diabetes Mellitus Using Alloxan in Sprague Dawley Rats

Kim

2024

Cureus

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Purpose: The use of rodent models for diabetes, particularly with pancreatic islet transplantation, has been prevalent in various preclinical trials. The purpose of this study is to establish a diabetes mellitus (DM) model in Sprague Dawley (SD) rats using alloxan evaluated by assessing alloxan dosage, the induction rate of diabetes, and glucose stability through insulin treatment. Methods: Over the course of 13 experimental rounds, diabetes was induced in 86 SD rats using alloxan at concentrations of 200 mg/kg (16 rats) or 150 mg/kg (70 rats). Various parameters, including diabetes induction rates, average insulin doses, extent of weight loss, and adverse effects such as diabetic ketoacidosis (DKA), were measured. Results: The administration of 200 mg/kg of alloxan in rats resulted in severe diabetes induction, leading to DKA in three individuals, despite daily insulin glargine administration, DKA prevention was unsuccessful. The stability of alloxan decreases over time, especially when refrigeration is compromised during weighing. In the group treated with 150 mg/kg of alloxan, the diabetes induction rate was 83%. The average insulin dose was 2.21 units/kg/day. In contrast, the group treated with 200 mg/kg of alloxan exhibited a diabetes induction rate of 81% with a statistically significant higher average insulin requirement at 7.58 units/kg/day compared to 150 mg/kg of alloxan. Conclusion: Inducing diabetes in rats with 150 mg/kg of alloxan is considered more suitable for creating a diabetes model for xenogeneic islet transplantation compared to using 200 mg/kg of alloxan. This is due to fewer complications related to DKA or hyperglycemia and reduced need for exogenous insulin treatment.

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RANKL/RANK contributes to the pathological process of type 2 diabetes mellitus through TRAF3 activation of NIK

Yu,

Tian,

et al. 2024

International Immunopharmacology

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Regeneration of islet β‐cells in tree shrews and rats

Cited by 4 publications

References 37 publications

Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic rats

Whey protein enriched with Stevia rebaudiana fraction restores the pancreatic function of streptozotocin induced diabetic rats

Induction of Diabetes Mellitus Using Alloxan in Sprague Dawley Rats

RANKL/RANK contributes to the pathological process of type 2 diabetes mellitus through TRAF3 activation of NIK

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