Regeneration of Kappaphycus alvarezii seaweed resulting from the transformation of the PaCs gene (Citrate Synthase) to increase resistance to environmental congestion in floating net cages in Pangkep water

Abstract

The PaCs (Citrate Synthase) gene of the seaweed K. alvarezii has effectively undergone in vitro transformation. To improve the survival of the transformation of the Citrate Synthase seaweed gene, it is carried out: (1) Regeneration of the results of transformation in culture chamber in the laboratory; (2) Acclimatization in green net (MS 100 μm) size 50x50x50 cm, density 200 explants maintained for 2 weeks, transferred to the blue net with a size of 50x50x50 cm (MS 200 μm) density 200 explants maintained for 4 weeks, then maintenance by the longline method using a double rope with a distance of 15 Cm on floating net cages. At intervals of two weeks, measurements of size, length, and water quality are made. Analysis of PaCs gene integration with PCR using Primary PaCS-F and PaCS-R. The results showed that the long DGR in the culture chamber was 0.11% to 0.18%/day, weight growth rate 0.62-1.30%/day, Acclimatization on green nets indicates a long growth rate of 2.28-2.3%/day, weight grow rate of 0.62 to 1.3% / day, and on blue nets, increases long grow rate 3-5%/day and weight 1.15-2.18%/day. Seaweed enlargement using the longline method shows a long growth rate of 5.3-9%/day and weight growth range from 1,23-3.2%/day. The band was located in the CaMV 35S vector at a place corresponding to the 1630 bp PaCS-F and PaCS-R promoter segments, according to the results of the integration of the Citrate Synthase gene in seaweed. Based on these findings, the seaweed K. alvarezii has successfully received the PaCS gene insertion.