Regeneration of plants from callus tissue of okra (Abelmoschus esculentus)

Roy, Mohashweta; Mangat, B. S.

doi:10.1016/0168-9452(89)90046-0

Cited by 18 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding in vitro culture of okra, only limited numbers of protocols were reported for shoot organogenesis (Table 2 ). Tissue culture-based direct shoot regeneration from cotyledon and cotyledonary node explants (Mangat and Roy, 1986 ), and regeneration of okra plants from callus tissue derived from cotyledonary axil (Roy and Mangat, 1989 ) are known. Further, Ganesan et al ( 2007 ) have reported plant-regeneration protocol through somatic embryogenesis from suspension culture; while Anisuzzaman et al ( 2008a ) developed a protocol for mass in vitro propagation using meristem culture for disease-free plant production.…”

Section: Transformation Protocols and Transgenic Development In Okramentioning

confidence: 99%

Biotechnological Advancements and Begomovirus Management in Okra (Abelmoschus esculentus L.): Status and Perspectives

et al. 2017

View full text Add to dashboard Cite

Despite the importance of okra, as one of the important vegetable crop, very little attention has been paid to its genetic improvement using advanced biotechnological tools. The exploitation of marker assisted breeding in okra is often limited due to the availability of a few molecular markers, the absence of molecular genetic-map(s), and other molecular tools. Chromosome linkage-groups were not yet constructed for this crop and reports on marker development are very scanty and mostly hovering around cultivar characterization. Besides, very little progress has been observed for transgenic development. However, high throughput biotechnological tools like chromosome engineering, RNA interference (RNAi), marker-assisted recurrent selection (MARS), genome-wide selection (GWS), targeted gene replacement, next generation sequencing (NGS), and nanobiotechnology can provide a rapid way for okra improvement. Further, the etiology of many deadly viral diseases like the yellow vein mosaic virus (YVMV) and okra enation leaf curl virus (OELCV) in okra is broadly indistinct and has been shown to be caused by various begomovirus species. These diseases cause systemic infections and have a very effective mode of transmission; thus, preventing their spread has been very complicated. Biotechnological interventions have the potential to enhance okra production even under different viral-stress conditions. In this background, this review deals with the biotechnological advancements in okra per se along with the begomoviruses infecting okra, and special emphasis has been laid on the exploitation of advanced genomic tools for the development of resistant varieties.

show abstract

Section: Transformation Protocols and Transgenic Development In Okramentioning

confidence: 99%

Biotechnological Advancements and Begomovirus Management in Okra (Abelmoschus esculentus L.): Status and Perspectives

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In the present study on dahlia, nodular calli were induced from leaf explants by single application of one of the cytokinins, either BA, TDZ or zeatin, without co-addition of auxins. Occurrence of callus induction on only cytokinin-containing medium has been reported in several species such as Stylosanthes scabra (Godwin et al 1987) and Cuscuta reflexa (Das et al 2011) with BA and okra (Abelmoschus esculentus) with either BA, kinetin or zeatin (Roy and Mangat 1989). In dahlia, almost 100% of the leaf explants showed nodular callus formation on medium with 10 mg l −1 TDZ or zeatin.…”

Section: Discussionmentioning

confidence: 99%

Highly efficient system for plant regeneration from leaf and stem explants in Dahlia

Otani

Endo

Chin

et al. 2013

Plant Biotechnology

View full text Add to dashboard Cite

Protocol of plant regeneration from leaf and stem explants was established in dahlia (Dahlia×pinnata) 'Yamatohime' in in vitro culture. Nodular calli were successfully induced from leaf and stem explants on 30 g l −1 sucrosecontaining MS medium supplemented singly with one of the cytokinins, 6-benzyladenine (BA), zeatin or thidiazuron (TDZ) and the highest frequency of nodular callus induction was obtained on medium containing 10 mg l −1 TDZ. The nodular callus induced on this medium subsequently produced a tissue with numerous shoot primordial structures called 'mass of shoot primordia' (MSP), which was maintained at least for more than 2 years on the same medium. Shoot induction occurred after transfer the MSP onto hormone-free MS medium, in which replacement of sucrose by maltose was necessary. The shoots excised from the MSP were rooted on hormone-free MS medium and successfully grown under greenhouse conditions. The regenerated plants showed the same morphological characteristics to those of the control plants and flowered normally.

show abstract

“…51 In another study on A. esculentus, callus was induced from hypocotyl and cotyledonary axil explants on kinetinsupplemented MS medium and rejuvenation of plants by supplementing benzyl adenine was observed. 48 Following this, Haider et al(1993) also regenerated the wholeplant from the hypocotyl callus culture with a combination of BAP and NAA treatment. 49 Ganesan et al (2007) have also reported a tissue culture study on A. esculentus, they devised a method for generating somatic embryos and regenerating plants using hypocotyl explants.…”

Section: Biotechnological Studies On a Esculentus Tissue Culture Studiesmentioning

confidence: 99%

Advances in the Micropropagation and Genetic Transformation of Abelmoschus Esculentus (L.) Moench for Insect Resistance

Daniel¹,

Duraipandiyan²,

Packiam³

2023

Curr Agri Res Jour

View full text Add to dashboard Cite

Abelmoschus esculentus (L.) Moench, known as okra, is a common vegetable crop in many diets and serves as a nutrient-rich source. It has a high content of protein, vitamins, minerals and compounds of high medicinal value. India tops in the consumption of pods and ranks first among the worldwide total production. It is now widely cultivated in many countries. Among the factors that hamper okra's marketable fruit yield, insect pests are the major ones. As numerous pests attack vegetables, controlling insect pests is one of the key elements to improve the yield of this crop. A workable approach for improving okra yield is micropropagation. It has been employed for a variety of things, including as large multiplication, inducing somaclonal variation to improve the desirable agronomic traits, maintaining certain genotypes, and genetic modification utilising molecular techniques. In this review, we highlight the most significant research on the micropropagation of okra, which is mediated by a variety of regeneration responses. The media and growth regulators for each of the approaches discussed, we go through how transformation techniques for insect resistance have been made possible via micropropagation. Utilizing this technology might be a workable plan to add genes and enhance particular features. Studying molecular pathways is another option provided by genetic transformation. This offers benefits for developing breeding programmes and optimising field production especially the effective use of CRISPR in genetically diverse lepidopteran insects opened options to study gene functions, insect modification, and pest management.

show abstract

Regeneration of plants from callus tissue of okra (Abelmoschus esculentus)

Cited by 18 publications

References 15 publications

Biotechnological Advancements and Begomovirus Management in Okra (Abelmoschus esculentus L.): Status and Perspectives

Biotechnological Advancements and Begomovirus Management in Okra (Abelmoschus esculentus L.): Status and Perspectives

Highly efficient system for plant regeneration from leaf and stem explants in Dahlia

Advances in the Micropropagation and Genetic Transformation of Abelmoschus Esculentus (L.) Moench for Insect Resistance

Contact Info

Product

Resources

About