Regeneration of Plants From Strawberry (Fragaria X Ananassa Duch.) Unpollinated Ovaries and Petals

Production of Gynogenic Tetrahaploid Plants in Strawberry Through Culture of Unpollinated Ovaries

“…After putting the cultures (explants cultured in jars of media) in the dark for 2-3 weeks at 25˚C, the optimum percentage of responding ovaries was achieved with MGO medium (Table 2) associated with zero cold pre-treatment which, significantly, were the best conditions for the two studied cultivars. These results agree with those of Predieri et al (1989) who obtained regeneration from the ovaries of strawberry without cold pre-treatment, and also with Campion and Alloni (1990) and Bohanec et al (1995) using unpollinated onion ovules. The same medium, i.e., MGO medium associated with 1 h cold pre-treatment also gave a high response for both cvs.…”

“…The results agree with those of Bohanec et al (1995) who obtained high regeneration frequency from onion ovaries. Usage of BA agree with reports of Bossoutrot and Hosemans (1985) on Beta vulgaris L. unpollinated ovules, Predieri et al (1989) on strawberry ovaries, Owen and Miller (1996) and Mohamed et al (2002) on strawberry anther culture. Also, usage of BA, IBA, and GA3 confirmed results obtained by Passey et al (2003) who obtained 12 % regeneration frequency from prepollinated ovaries of the strawberry cv.…”

“…Dark-light period was important for regeneration of strawberry anther culture to produce more shoots (Owen and Miller, 1996) and squash anther culture (Metwally et al, 1998), meanwhile, Bossoutrot and Hosemans (1985) produced gynogenic plants in Beta vulgaris L. from in vitro culture of unpollinated ovules without dark treatment. On the contrary, Predieri et al (1989) found that the dark has negative effect on strawberry ovaries culture. The MGO medium showed increase in responding ovaries, which may be due to its components of lower macro nutrients, higher organic and inorganic nitrogen, higher vitamins, usage of BA, IBA and GA3, and two different sources of sugar (sucrose and glucose).…”

“…A.10-13). Predieri et al (1989) reported that most regeneration of strawberry ovaries originated from the area where the ovary had been detached from the receptacle and the internal parenchymatic ovary tissues close to the ovule and that was obvious through the histological examinations. After this period in dark the cultures were exposed to light (16 h/d photoperiod) for 3-4 weeks using the same media.…”

“…For producing gynogenic plants in vegetable crops, Bossoutrot and Hosemans (1985) produced gynogenic plants in Beta vulgaris L. from in vitro culture of unpollinated ovules. Predieri et al (1989) obtained regenerated plants from strawberry unpollinated ovaries, but they did not count the chromosome number of these plants. Campion and Alloni (1990), Bohanec et al (1995), and Alan et al (2007) induced haploid plants in onion from in vitro culture of unpollinated ovaries.…”