Regeneration of the aquatic monocot Aponogeton madagascariensis (lace plant) through callus induction

Carter, Joey; Gunawardena, Arunika H. L. A. N.

doi:10.1016/j.aquabot.2011.01.005

Cited by 13 publications

(11 citation statements)

References 20 publications

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“…However, in other sources such as leaves, petioles and root tips of A. ulvaceus , lethal browning of tissue was one of the impediments to callus induction. Our findings are in conformity with the results of Carter and Gunawardena ( 2011 ), who observed no callus formation in petioles and root tips of A. madagascariens due to browning. Such phenomenon can be attributed to the exudation and oxidation of phenolic compounds in the culture medium as a defence response following tissue wounding or stress (Jones and Saxena 2013 ).…”

Section: Discussionsupporting

confidence: 94%

“…Immature tubers with meristems obtained from in vitro-raised plantlets were used as explant materials. Tuber explants were longitudinally dissected into four equal sections and cultured on MS medium supplemented with different concentrations of BAP (0, 1, 2, and 3 mg/L) in combination with NAA (0, 1, 2 and 3 mg/L) for callus induction and subsequent regeneration (Carter and Gunawardena 2011 ). To prevent desiccation, liquid MS was poured over the explants to submerge the entire tuber segment.…”

Section: Methodsmentioning

confidence: 99%

“…To date, numerous experimental studies on aquatic plant tissue culture have been reported. Aquatic plant species that have been successfully propagated in vitro include A. madacasgariensis (Carter and Gunawardena 2011 ), Cryptocoryne wendtii (Stanly et al 2011 ), Hygrophila polysperma (Roxb.) T. Anderson (Çinar et al 2013 ), Myriophyllum spicatum L. (Zhou et al 2006 ), Nymphoides indica (Jenks et al 2000 ), Pogostemon helferi (Wangwibulkit and Vajrodaya 2016 ) and Potamogeton crispus L. (Zhou et al 2006 ).…”

Section: Introductionmentioning

confidence: 99%

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The biology and in vitro propagation of the ornamental aquatic plant, Aponogeton ulvaceus

2016

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Aponogeton ulvaceus Baker (Aponogetonaceae) is a commercially important ornamental aquatic plant species with traditional medicinal uses. Due to the low survival rate of seedlings, propagation by conventional means has been met with many difficulties. In this study, botanical aspects of A. ulvaceus were examined with regards to the morphology, anatomy and physiology of the plant and an efficient protocol for its in vitro propagation using immature tuber explants has been established. The existence of glandular trichomes on the leaves was discovered and the occurrence of circumnutation in A. ulvaceus has been demonstrated. Immature tuber segments with meristems were cultured on MS medium supplemented with various combinations (0, 1, 2, and 3 mg/L) of BAP and NAA for callus induction. The highest percentage of callus production (100 %) was obtained in two different treatments: 1 mg/L BAP and 3 mg/L NAA, and 2 mg/L BAP and 3 mg/L NAA. For shoot and root organogenesis, the combination of 1 mg/L BAP and 1 mg/L NAA was shown to be significant for A. ulvaceus regeneration when compared to control, which yields a mean shoot and root number of 22.50 and 29.50 respectively. The current protocol is the first reported successful establishment of in vitro clonal propagation of A. ulvaceus.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-016-3041-4) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The biology and in vitro propagation of the ornamental aquatic plant, Aponogeton ulvaceus

2016

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“…Figure one C [1] had been previously published as Figure one C in [2]; additionally Figure one F [1] had been previously published as Figure one E in [3]. This duplication of figures was inadvertent and we have now received permission from both publishers (Global Science Books [2] and Elsevier [3]) to reproduce these images. Note that these figures were for introductory demonstration purposes only, and this correction does not affect the scientific content of the article.…”

Section: Correctionmentioning

confidence: 99%

Erratum to: Do mitochondria play a role in remodelling lace plant leaves during programmed cell death?

et al. 2013

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“…In vitro tissue culture has been identified as an effective technique for large scale multiplication of elite plants. Several reports have demonstrated that aquatic plants can be multiplied by in vitro propagation through proliferation from pre-existing bud in Cryptocoryne, Anubias, Myriophyllum, andPotamogeton (Kane et al, 1990, 1999;Huang et al, 1994;Zhou et al, 2006;Kanchanapoom et al, 2012), through adventitious shoot formation in Limnophila and Aponogeton (Rao and Mohan Ram, 1981;Carter and Gunawardena, 2011) or embryogenesis in Scirpus and Nymphoides (Wang et al, 2004;Myung et al, 2010). However, the most suitable micropropagation method for each species which are commercially common in Japan is still uncertain.…”

Section: Introductionmentioning

confidence: 99%

Niki¹,

Amaki²

2014

Acta Hortic.

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Three aquatic plants, Glossostigma elatinoides (Benth.) Hook.f., Limnophila sp. (unidentified), and Microcarpaea minima (K.D. Koenig ex Retz.) Merrill. were examined to clarify the optimal culture medium and cultural conditions for their micropropagation. Murashige and Skoog (MS) medium strength (1/1, 1/2, 1/4 and 1/8), sucrose concentration (0, 10, 20, 30, and 40 g•L-1), initial pH of medium (4.5, 5.0, 5.5, 6.0, and 6.5), and the kind and concentration of gelling agent (6, 7, 8, 9, 10 g•L-1 for agar and 3 g•L-1 for gellan gum) were examined. The optimal medium conditions for all of the examined plants was ½ MS, 20 g•L-1 sucrose, and 3 g•L-1 gellan gum. The optimal medium pH was 5.0 for G. elatinoides and 6.0 for M. minima and Limnophila. Water supplement during culture was effective on the growth and acclimatization of multiplied plants. The optimal timing of supplement was after 20 days for G. elatinoides and 30 days for M. minima and Limnophila from the explant inoculation.

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Regeneration of the aquatic monocot Aponogeton madagascariensis (lace plant) through callus induction

Cited by 13 publications

References 20 publications

The biology and in vitro propagation of the ornamental aquatic plant, Aponogeton ulvaceus

The biology and in vitro propagation of the ornamental aquatic plant, Aponogeton ulvaceus

Erratum to: Do mitochondria play a role in remodelling lace plant leaves during programmed cell death?

Micropropagation of Ornamental Aquatic Plants, Glossostigma, Microcarpaea, and Limnophila©

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