Regeneration of Three Sweet Potato (Ipomoea batatas (L.)) Accessions in Ghana via, Meristem and Nodal Culture

Addae-Frimpomaah, F.; Amponsah, Jonathan; Tengey, Theophilus Kwabla

doi:10.3923/ijpbg.2014.121.138

Cited by 10 publications

(11 citation statements)

References 8 publications

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“…Plant hormones exert profound morphological effects on plant growth and development, the precise action depending on the type and concentration of the hormone and the sensitivity of the organ involved [13]. In this study, the effect of BAP, GA3 and NAA, either in combination or alone, on cultured Brondal meristems was investigated.…”

Section: Discussionmentioning

confidence: 99%

Effect of Bap, Naa and Ga3, Either Alone or in Combination, on Meristem Culture and Plantlet Establishment in Sweet Potato (Cv Brondal)

Masekesa¹,

Gasura²,

Matikiti³

et al. 2016

AJFAND

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In Zimbabwe, the average sweet potato yield (6 t/ha) is relatively low when compared to Asian counterparts (17 t/ha). These low crop yields have been blamed on weevil infestations and viral infections which account for 60-90% of sweet potato yield losses in Africa. Meristem tip culture, a Centre for Potato Improvement (CIP) initiated tissue culture technique, has been widely used to eradicate viruses from clonally propagated crops and has been noted to be one of the instrumental techniques that helped China to increase sweet potato yields. In an effort to adopt the meristem tip culture technique for the production of virus-free planting material of a local sweet potato (cv Brondal), a study was conducted to evaluate the effect of Benzylamino purine (BAP), 1-Naphthaleneacetic acid (NAA) and Gibberellic acid (GA3) (either alone or in combination) on cultured Brondal meristems. The different hormonal treatments were assessed on the following parameters: plantlet regenerative capacity, multiple plantlet production, shoot height, average leaf number per shoot and average node number per shoot, ten weeks after meristem culture. All treatments containing a combination of BAP (1 mg -L) and GA3 (at either 5 mg -L, 10 mg -L, or 20 mg -L) had a significantly (p<0.01) higher plantlet regenerative capacity of 33-66% when compared to other treatment combinations. Only treatments, 10 mg -L GA3 + 1 mg -L BAP and 20 mg -L GA3 + 1 mg -L BAP were capable of inducing multiple plantlet formation, producing an average of three plantlets/meristem and two plantlets/meristem respectively. Overall, treatment 10 mg -L GA3 + 1 mg -L BAP gave rise to significantly (p<0.01) taller shoots (20 mm) compared to the rest of the treatments used. For average leaf number per shoot, all GA3 treatments (5 mg -L, 10 mg -L, or 20 mg -L) supplemented with 1 mg -L BAP gave significantly (p<0.01) higher numbers of leaves (six leaves/shoot) than the rest of the treatments. Treatments 10 mg -L GA3 + 1 mg -L BAP and 20 mg -L GA3 + 1 mg -L BAP gave rise to the highest number of nodes per shoot, producing an average of three nodes per shoot. In sharp contrast to treatments containing a combination of BAP and GA3, all treatments containing a combination of BAP and NAA performed poorly in all parameters tested for plant regeneration of Brondal sweet potato variety. In conclusion, the best hormonal treatment for culturing Brondal meristems proved to be 10 mg -L GA3 + 1 mg -L BAP.

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Section: Discussionmentioning

confidence: 99%

Effect of Bap, Naa and Ga3, Either Alone or in Combination, on Meristem Culture and Plantlet Establishment in Sweet Potato (Cv Brondal)

Masekesa¹,

Gasura²,

Matikiti³

et al. 2016

AJFAND

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“…This media also recorded the least mean value in all the measured parameters except for shoot height and degree of callusing. Addae-Frimpomaah et al (2014) obtained callus in media supplemented with low concentration of BAP or kinetin (KIN). They reported 0.25 mg/l BAP to have given the highest result of shoot induction (80%).…”

Section: Fig-2: Callus and Plantlets From Some Of The Media Combinationsmentioning

confidence: 99%

In vitro propagation of sweet potato (Ipomoea batatas (L.) Lam.) cultivars

Abubakar

Yahaya

Shaibu

et al. 2018

ASD

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The study is aimed at establishing a simple protocol for in vitro regeneration of sweet potato with a view to providing planting materials to farmers as well as basis for genetic improvement. Axillary buds were excised and cultured on Murashige and Skoog (MS) basal salts supplemented with 6-benzyl aminopurine (BAP), gibberellic acid (GA 3 ) and naphthalene acetic acid (NAA) singly or in combination. The shoot height and number of leaves differed significantly among the cultivars. The result also indicated significant difference (p< 0.01) among the cultivars with King J recording the highest mean values. Significant differences (p< 0.05) was also recorded in the media combination with respect to organogenesis and number of shoots obtained. The results of hardening further revealed 33.33% success in the explants transferred directly to the field, as well as for the plantlets that were gradually weaned in a mixture of 3:1 sand and biochar.

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“…Reports of similar research by others [3,6,10] showed that sweet potato is recalcitrant to every in vitro culture treatment and occur at low frequencies for various explants. In fact, shoots can easily be regenerated from explants of stems, petioles, leaves and roots but callus induction and shoot regeneration is difficult, if not impossible [11].…”

Section: Plantlet Regeneration Potential Of Callimentioning

confidence: 89%

“…Comparison of several protocols on regeneration of sweet potato [6,8] also showed that every cultivar vary widely in their responses to issue culture and plant regeneration [9]. Hence, genotype is one of the most important factors affecting the evolution of in vitro culture and regeneration [10]. Reported in this paper is an approach in which different explants of three sweet potato cultivars were used to induce embryogenic callus and attempts made to regenerate plantlets in vitro.…”

Section: Introductionmentioning

confidence: 99%

Responses of Different Explants of Sweet Potato on Modified MS and LS Based Nutrient Media in vitro

Michael

2019

AJAAR

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A good number of reports on sweet potato (Ipomoea batatas L.) tissue culture using different tissues of various cultivars with varying level of efficiency and reproducibility are available but callus induction and plantlet regeneration are recalcitrant and limited to a few genotypes in response to different treatments in vitro. Reported in this paper is a procedure in which several explants of three high yielding and drought tolerant sweet potato cultivars (SK010, WHCH005 and PRAP496), mostly grown and cultivated in the highlands of Papua New Guinea (PNG) were used to induce embryogenic callus and regenerate plantlets. To achieve a reliable and an efficient system for inducing embryogenic callus on stem, petiole and leaf disc explants, a modified form of Murashige and Skoog (MS) and Linsmaier and Skoog (LS) media, supplemented with 1 gL-1 picloram and 8 gL-1 agar was used. This procedure resulted in large amount of embryogenic calli that were potentially capable of regenerating whole plantlets on all the different types of explants tested. Further attempts made in plantlet regeneration failed, however ways in which improvement of the tested regeneration media can be made for plant regeneration in similar studies were discussed.

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Regeneration of Three Sweet Potato (Ipomoea batatas (L.)) Accessions in Ghana via, Meristem and Nodal Culture

Cited by 10 publications

References 8 publications

Effect of Bap, Naa and Ga3, Either Alone or in Combination, on Meristem Culture and Plantlet Establishment in Sweet Potato (Cv Brondal)

Effect of Bap, Naa and Ga3, Either Alone or in Combination, on Meristem Culture and Plantlet Establishment in Sweet Potato (Cv Brondal)

In vitro propagation of sweet potato (Ipomoea batatas (L.) Lam.) cultivars

Responses of Different Explants of Sweet Potato on Modified MS and LS Based Nutrient Media in vitro

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