Regeneration of Zebrafish CNS: Adult Neurogenesis

Ghosh, Sukla; Hui, Subhra Prakash

doi:10.1155/2016/5815439

Cited by 70 publications

(56 citation statements)

References 155 publications

(211 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The zebrafish is a powerful model organism for several reasons: (i) transparent embryos develop quickly ex utero facilitating longitudinal live imaging with microscopy throughout heart development, (ii) inexpensive and large numbers of subjects, and (iii) ease of high-throughput screening paired with sophisticated genetic approaches. Zebrafish as a model of brain development have rapidly increased in popularity over the past two decades and are now being used in a wide variety of studies modeling cognitive disorders, emotional and behavioral motivation, myelination, regeneration, autism, and neuropharmacological screening 136–139 .…”

Section: Animal Models In Chdmentioning

confidence: 99%

Neurodevelopmental Abnormalities and Congenital Heart Disease

Morton

Ishibashi

Jonas

2017

Circulation Research

158

View full text Add to dashboard Cite

In the past two decades it has become evident that individuals born with congenital heart disease (CHD) are at risk of developing life-long neurological deficits. Multifactorial risk factors contributing to neurodevelopmental abnormalities associated with CHD have been identified; however the underlying etiologies remain largely unknown and efforts to address this issue have only recently begun. There has been a dramatic shift in focus from newly acquired brain injuries associated with corrective and palliative heart surgery to antenatal and preoperative factors governing altered brain maturation in CHD. In this review, we describe key time windows of development during which the immature brain is vulnerable to injury. Special emphasis is placed on the dynamic nature of cellular events and how CHD may adversely impact the cellular units and networks necessary for proper cognitive and motor function. In addition, we describe current gaps in knowledge and offer perspectives about what can be done to improve our understanding of neurological deficits in CHD. Ultimately, a multidisciplinary approach will be essential in order to prevent or improve adverse neurodevelopmental outcomes in individuals surviving CHD.

show abstract

Section: Animal Models In Chdmentioning

confidence: 99%

Neurodevelopmental Abnormalities and Congenital Heart Disease

Morton

Ishibashi

Jonas

2017

Circulation Research

158

View full text Add to dashboard Cite

show abstract

“…Zebrafishes are an excellent model to target functional nerve regeneration in humans and other mammals (Ghosh & Hui, 2016). These fishes have an innate ability to regenerate; however, the time taken for regeneration increases with aging (Graciarena, Dambly-Chaudiere, & Ghysen, 2014).…”

Section: Animal Experimentsmentioning

confidence: 99%

Strategic design of peptide‐decorated aligned nanofibers impregnated with triiodothyronine for neural regeneration

Satish

Korrapati

2019

J Tissue Eng Regen Med

View full text Add to dashboard Cite

Nerve injuries are often debilitating as its regeneration occurs in a slow and laborious manner. Remediation of nerve injury is a colossal task as functional restoration in larger gaps seldom occurs due to the complex nerve regeneration mechanism. A nanofiber‐based graft material has been fabricated to provide topographical and biochemical cues to encourage neural differentiation. Laminin plays a crucial role in supporting peripheral nerve regeneration and hence aligned polyvinyl cinnamate nanofibers surface‐conjugated with laminin‐derived cell‐adhesion peptides have been fabricated to improve selective neural adhesion and regeneration. Further, triiodothyronine has been encapsulated within the nanofibers enabling its sustained release so as to bolster regeneration and reinstate the lost functionality to the damaged nerve. The fabricated nanofibers were characterized for its physicochemical, morphological, and topographical properties. Nanofibers were biocompatible, improved cell adhesion rate, and illustrated favourable interaction with cells. Gene expression (showed 9.5 and 4.1 fold increase in β‐tubulin and MAP 2 expression, respectively) and protein expression (immunofluorescence, flow cytometry, and western blot) studies confirmed the positive influence of the scaffold over cell differentiation. The studies were extrapolated to adult zebrafish model with a surgical incision in posterior lateral line. The biocomposite treated group showed earlier functional restoration of the nerve compared with control groups detected by touch‐evoked response. Thus, the combination of aligned nanofibers providing topographical cue, along with the peptides and triiodothyronine serving as biochemical cues, has a robust potential to restore functionality to the injured nerve, thereby opening avenues for fabrication of regenerative nerve grafts.

show abstract

“…The retinas of amphibians and teleost fish have an unprecedented capacity to regenerate their retina after damage, demonstrating the existence of stem and progenitor cells in the eye [11–13]. Following damage, retinal cells express genes typical for embryonic retinal progenitors such as Pax6, Notch-3, and n-cadherin [14], and the relative spatiotemporal patterns of expression of these genes mimic the patterns observed during retinal neurogenesis.…”

Section: Intrinsic Retinal Regeneration In Vertebratesmentioning

confidence: 99%

Recent Advances in Retinal Stem Cell Therapy

2017

View full text Add to dashboard Cite

Purpose of review Progress in stem cell research for blinding diseases over the past decade is now being applied to patients with retinal degenerative diseases and soon perhaps, glaucoma. However, the field still has much to learn about the conversion of stem cells into various retinal cell types, and the potential delivery methods that will be required to optimize the clinical efficacy of stem cells delivered into the eye. Recent findings Recent groundbreaking human clinical trials have demonstrated both the opportunities and current limitations of stem cell transplantation for retinal diseases. New progress in developing in vitro retinal organoids, coupled with the maturation of bio-printing technology, and non-invasive high-resolution imaging have created new possibilities for repairing and regenerating the diseased retina and rigorously validating its clinical impact in vivo. Summary While promising progress is being made, meticulous clinical trials with cells derived using good manufacturing practice, novel surgical methods, and improved methods to derive all of the neuronal cell types present in the retina will be indispensable for developing stem cell transplantation as a paradigm shift for the treatment of blinding diseases.

show abstract

Regeneration of Zebrafish CNS: Adult Neurogenesis

Cited by 70 publications

References 155 publications

Neurodevelopmental Abnormalities and Congenital Heart Disease

Neurodevelopmental Abnormalities and Congenital Heart Disease

Strategic design of peptide‐decorated aligned nanofibers impregnated with triiodothyronine for neural regeneration

Recent Advances in Retinal Stem Cell Therapy

Contact Info

Product

Resources

About