Regenerative Medicine and Hair Loss: How Hair Follicle Culture has Advanced our Understanding of Treatment Options for Androgenetic Alopecia

Christiano, Angela M.

doi:10.2217/rme.13.87

Cited by 26 publications

(25 citation statements)

References 97 publications

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“…Cultured rat DPCs can self-aggregate to formed a papilla-like clump after subdermal injection, but spontaneous aggregation of cultured human DPCs injected into skin has not been observed 40, 41 . It has been shown that very rapid and extensive changes in molecular signature are associated with the transition of human DPCs from a three-dimensional to a two-dimensional environment, and that DPCs tend to lose their trichogenic ability when grown as traditional two-dimensional monolayers 42, 43 . Higgen et al .…”

Section: Discussionmentioning

confidence: 99%

As a carrier–transporter for hair follicle reconstitution, platelet-rich plasma promotes proliferation and induction of mouse dermal papilla cells

Xiao

Miao

Wang

et al. 2017

Sci Rep

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Morphogenesis of hair follicles during development and in hair reconstitution assays involves complex interactions between epithelial cells and dermal papilla cells (DPCs). DPCs may be a source of cells for hair regeneration in alopecia patients. Reconstitution of engineered hair follicles requires in vitro culture of trichogenic cells, a three-dimensional scaffolds, and biomolecular signals. However, DPCs tend to lose their biological activity when cultured as trichogenic cells, and scaffolds currently used for hair follicle regeneration lack biological efficiency and biocompatibility. Platelet-rich plasma (PRP) gel forms a three-dimensional scaffold that can release endogenous growth factors, is mitogenic for a variety of cell types and is used in model tissue repair and regeneration systems. We found that 5% activated PRP significantly enhanced cell proliferation and hair-inductive capability of mouse and human DPCs in vitro and promoted mouse hair follicle formation in vivo. PRP also formed a three-dimensional gel after activation. We used PRP gel as a scaffold to form many de novo hair follicles on a plane surface, showing it to be candidate bioactive scaffold capable of releasing endogenous growth factors for cell-based hair follicle regeneration.

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Section: Discussionmentioning

confidence: 99%

As a carrier–transporter for hair follicle reconstitution, platelet-rich plasma promotes proliferation and induction of mouse dermal papilla cells

Xiao

Miao

Wang

et al. 2017

Sci Rep

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“…The compartmentalized expression of pro-and antiapoptotic factors in the HF is shown in Figure 3. A diverse array of additional molecules have been found to play a role in catagen induction, including FGF-5 [26,27], interferon (IFN)-g [28], substance P [29], and estrogens [30]. The apoptotic processes within the HF are also controlled by caspases 21, 23, 24, and 27 [21, 31, 32], and can also be triggered by the withdrawal of DP-derived growth factors or by apoptotic signals produced by mast cells located within the CTS [29,33,34].…”

Section: Catagenmentioning

confidence: 99%

A Clinical and Biological Guide for Understanding Chemotherapy-Induced Alopecia and Its Prevention

et al. 2017

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Chemotherapy-induced alopecia (CIA) represents perhaps the most distressing side effect of chemotherapeutic agents and is of huge concern to the majority of patients. Scalp cooling is currently the only safe option to combat CIA. Clinical and biological evidence suggests improvements can be made, including efficacy in delivering adequately low temperature to the scalp and patient-specific cap design. The increased use of scalp cooling, an understanding of how to deliver it most effectively, and biological evidence-based approaches to improve its efficacy have enormous potential to ease the psychological burden of CIA, as this could lead to improvements in treatment and patient quality-of-life.

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“…The HF organ culture model is commonly used to determine whether small molecules or biologics may have a hair growth-promoting effect [69]. The advantage is that the effect of a compound can be assessed simultaneously on the epithelial and mesenchymal compartment of the HF.…”

Section: Androgenic Alopeciamentioning

confidence: 99%

Drug discovery for alopecia: gone today, hair tomorrow

Santos

Avci

Hamblin

2015

Expert Opinion on Drug Discovery

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Introduction Hair loss or alopecia affects the majority of the population at some time in their life, and increasingly, sufferers are demanding treatment. Three main types of alopecia (androgenic [AGA], areata [AA] and chemotherapy-induced [CIA]) are very different, and have their own laboratory models and separate drug-discovery efforts. Areas covered In this article, the authors review the biology of hair, hair follicle (HF) cycling, stem cells and signaling pathways. AGA, due to dihydrotesterone, is treated by 5-α reductase inhibitors, androgen receptor blockers and ATP-sensitive potassium channel-openers. AA, which involves attack by CD8+NK group 2D-positive (NKG2D+) T cells, is treated with immunosuppressives, biologics and JAK inhibitors. Meanwhile, CIA is treated by apoptosis inhibitors, cytokines and topical immunotherapy. Expert opinion The desire to treat alopecia with an easy topical preparation is expected to grow with time, particularly with an increasing aging population. The discovery of epidermal stem cells in the HF has given new life to the search for a cure for baldness. Drug discovery efforts are being increasingly centered on these stem cells, boosting the hair cycle and reversing miniaturization of HF. Better understanding of the molecular mechanisms underlying the immune attack in AA will yield new drugs. New discoveries in HF neogenesis and low-level light therapy will undoubtedly have a role to play.

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Regenerative Medicine and Hair Loss: How Hair Follicle Culture has Advanced our Understanding of Treatment Options for Androgenetic Alopecia

Cited by 26 publications

References 97 publications

As a carrier–transporter for hair follicle reconstitution, platelet-rich plasma promotes proliferation and induction of mouse dermal papilla cells

As a carrier–transporter for hair follicle reconstitution, platelet-rich plasma promotes proliferation and induction of mouse dermal papilla cells

A Clinical and Biological Guide for Understanding Chemotherapy-Induced Alopecia and Its Prevention

Drug discovery for alopecia: gone today, hair tomorrow

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