Regenerative MRL/MpJ Tendon Cells Exhibit Sex Differences in Morphology, Proliferation, Mechanosensitivity, and Cell-Matrix Remodeling

Abstract

Clinical and animal studies have reported the influence of sex on the incidence and progression of tendinopathy, which results in disparate structural and biomechanical outcomes. However, there remains a paucity in our understanding of the sex-specific biological mechanisms underlying effective tendon healing. To overcome this hurdle, our group has investigated the impact of sex on tendon regeneration using the super-healer Murphy Roths Large (MRL/MpJ) mouse strain. Despite a shared scarless healing capacity, we have shown that MRL/MpJ patellar tendons exhibit sexually dimorphic regulation of gene expression for pathways involved in fibrosis, cell migration, and extracellular matrix (ECM) remodeling following an acute midsubstance injury. Moreover, we previously found decreased matrix metalloproteinase-2 (MMP-2) activity in female MRL/MpJ tendons after injury. Thus, we hypothesized that MRL/MpJ scarless tendon healing is mediated by sex-specific and temporally distinct orchestration of cell-ECM interactions. Accordingly, the present study comparatively evaluated MRL/MpJ tendon cells under two-dimensional (glass) and three-dimensional (nanofiber scaffolds) culture platforms to examine cell behavior under biochemical and biophysical cues associated with tendon homeostasis and healing. Female MRL/MpJ cells showed reduced 2D migration and spreading area accompanied with enhanced mechanosensing, 2D ECM alignment, and fibronectin-dependent cell proliferation. Interestingly, female MRL/MpJ cells cultured on 3D isotropic scaffolds showed diminished ECM deposition and alignment. Regardless of culture condition and sex, MRL/MpJ cells outperformed B6 cells and elicited a universal regenerative cellular phenotype. These results illustrate the utility of these in vitro systems for elucidating regenerative tendon cell biology, which will facilitate the long-term development of more equitable therapeutics.

Proteins Derived From MRL/MpJ Tendon Provisional Extracellular Matrix and Secretome Promote Pro-Regenerative Tenocyte Behavior

“…We have recently shown that adult male and female MRL/MpJ tendons both demonstrate a regenerative capacity with vastly improved mechanical recovery compared to those of B6 mice (13). More recently, we also found that both male and female MRL/MpJ tenocytes exhibit similar or superior in vitro outcome measures, as detailed in the present study, compared to B6 tenocytes (64). These findings suggest that the cellular and molecular mechanisms underlying MRL/MpJ tendon regeneration are largely conserved between sexes.…”

Detergent-Free Decellularization Preserves the Mechanical and Biological Integrity of Murine Tendon

“…Our recent work has shown that B6 and MRL/MpJ tendon cells cultured on commercial 3D nanofiber scaffolds elicit distinct cellular responses to anisotropic and isotropic surface topographies associated with homeostasis and wound healing, respectively. 50 To gain further insight into how the interplay between the biochemical and structural properties of the ECM regulate cell activity, future studies could augment these biomaterial-based approaches by applying the dECM as a surface coating.…”