Regenerative Neurogenesis from Neural Progenitor Cells Requires Injury-Induced Expression of Gata3

Kızıl, Çağhan; Kyritsis, Nikos; Dudczig, Stefanie; Kroehne, Volker; Freudenreich, Dorian; Kaslin, Jan; Brand, Michael

doi:10.1016/j.devcel.2012.10.014

Cited by 132 publications

(161 citation statements)

References 30 publications

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“…Neuroinflammation and immune cells including microglia, regenerative astrocytes, and leukocytes have emerged as factors regulating injury-induced neurogenesis (for review, see Kohman and Rhodes, 2013). After neuronal death, microglia migrate to the injury site to remove cell debris, whereupon they release proinflammatory cytokines and proproliferative factors (for review, see Smith et al, 2012;Chen et al, 2013) to promote regeneration (Molowny et al, 1995;Kizil et al, 2012). Thus, a neuroinflammatory response may mediate apoptotic neuron clean up during HVC regression and may promote the vVZ proliferation increase we observed.…”

Section: Abmentioning

confidence: 93%

Reactive Neurogenesis in Response to Naturally Occurring Apoptosis in an Adult Brain

Larson¹,

Thatra

Lee

et al. 2014

J. Neurosci.

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Neuronal birth and death are tightly coordinated to establish and maintain properly functioning neural circuits. Disruption of the equilibrium between neuronal birth and death following brain injury or pharmacological insult often induces reactive, and in some cases regenerative, neurogenesis. Many neurodegenerative disorders are not injury-induced, however, so it is critical to determine if and how reactive neurogenesis occurs under noninjury-induced neurodegenerative conditions. Here, we used a model of naturally occurring neural degradation in a neural circuit that controls song behavior in Gambel's white-crowned sparrows (Zonotrichia leucophrys gambelii) and examined the temporal dynamics between neuronal birth and death. We found that during seasonal-like regression of the song, control nucleus HVC (proper name), caspase-mediated apoptosis increased within 2 d following transition from breeding to nonbreeding conditions and neural stem-cell proliferation in the nearby ventricular zone (VZ) increased shortly thereafter. We show that inhibiting caspase-mediated apoptosis in HVC decreased neural stem-cell proliferation in the VZ. In baseline conditions the extent of neural stem-cell proliferation correlated positively with the number of dying cells in HVC. We demonstrate that as apoptosis increased and the number of both recently born and pre-existing neurons in HVC decreased, the structure of song, a learned sensorimotor behavior, degraded. Our data illustrate that reactive neurogenesis is not limited to injury-induced neuronal death, but also can result from normally occurring degradation of a telencephalic neural circuit.

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Section: Abmentioning

confidence: 93%

Reactive Neurogenesis in Response to Naturally Occurring Apoptosis in an Adult Brain

Larson¹,

Thatra

Lee

et al. 2014

J. Neurosci.

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“…These unexpected findings have revealed that distinct mechanisms control neurogenesis in intact and injured brains. A recent study using zebrafish has shown that injury-induced GATA3 plays a crucial role in regenerating neurons lost to insult (Kizil et al 2012). A similarity between GATA3 in zebrafish and Gsx2 in mice is that both are broadly up-regulated by injury and are essential for stimulation of neurogenesis.…”

Section: Mechanisms For Injury-induced Neurogenesismentioning

confidence: 99%

Gsx2 controls region-specific activation of neural stem cells and injury-induced neurogenesis in the adult subventricular zone

et al. 2013

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Neural stem cells (NSCs) reside in widespread regions along the lateral ventricle and generate diverse olfactory bulb (OB) interneuron subtypes in the adult mouse brain. Molecular mechanisms underlying their regional diversity, however, are not well understood. Here we show that the homeodomain transcription factor Gsx2 plays a crucial role in the region-specific control of adult NSCs in both persistent and injury-induced neurogenesis. In the intact brain, Gsx2 is expressed in a regionally restricted subset of NSCs and promotes the activation and lineage progression of stem cells, thereby controlling the production of selective OB neuron subtypes. Moreover, Gsx2 is ectopically induced in damaged brains outside its normal expression domains and is required for injuryinduced neurogenesis in the subventricular zone (SVZ). These results demonstrate that mobilization of adult NSCs is controlled in a region-specific manner and that distinct mechanisms operate in continuous and injuryinduced neurogenesis in the adult brain.

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“…Zebrafish are highly regenerative as adults, being equipped to regrow tissues such as amputated fins, injured retinae, transected optic nerves and spinal cord, lost heart muscle, brain, hair cells, pancreas, liver, skeletal muscle and kidney (Johnson and Weston, 1995;Bernhardt et al, 1996;Becker et al, 1997;Vihtelic and Hyde, 2000;Poss et al, 2002;Sadler et al, 2007;Ma et al, 2008;Jacoby et al, 2009;Moss et al, 2009;Diep et al, 2011;Andersson et al, 2012;Goldshmit et al, 2012;Kizil et al, 2012). Adult zebrafish present advantages for mechanistic studies as they are small, easy to raise in large numbers and accessible to cell transplantation and molecular genetic manipulations.…”

Section: Introductionmentioning

confidence: 99%

zebraflash transgenic lines for in vivo bioluminescence imaging of stem cells and regeneration in adult zebrafish

et al. 2013

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The zebrafish has become a standard model system for stem cell and tissue regeneration research, based on powerful genetics, high tissue regenerative capacity and low maintenance costs. Yet, these studies can be challenged by current limitations of tissue visualization techniques in adult animals. Here we describe new imaging methodology and present several ubiquitous and tissue-specific luciferase-based transgenic lines, which we have termed zebraflash, that facilitate the assessment of regeneration and engraftment in freely moving adult zebrafish. We show that luciferase-based live imaging reliably estimates muscle quantity in an internal organ, the heart, and can longitudinally follow cardiac regeneration in individual animals after major injury. Furthermore, luciferase-based detection enables visualization and quantification of engraftment in live recipients of transplanted hematopoietic stem cell progeny, with advantages in sensitivity and gross spatial resolution over fluorescence detection. Our findings present a versatile resource for monitoring and dissecting vertebrate stem cell and regeneration biology.

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Regenerative Neurogenesis from Neural Progenitor Cells Requires Injury-Induced Expression of Gata3

Cited by 132 publications

References 30 publications

Reactive Neurogenesis in Response to Naturally Occurring Apoptosis in an Adult Brain

Reactive Neurogenesis in Response to Naturally Occurring Apoptosis in an Adult Brain

Gsx2 controls region-specific activation of neural stem cells and injury-induced neurogenesis in the adult subventricular zone

zebraflash transgenic lines for in vivo bioluminescence imaging of stem cells and regeneration in adult zebrafish

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