Region-Specific Developmental Specialization of GABA–Glycine Cosynapses in Laminas I–II of the Rat Spinal Dorsal Horn

Keller, Anne Florence; Coull, Jeffrey A. M.; Chéry, Nadège; Poisbeau, Pierrick

doi:10.1523/jneurosci.21-20-07871.2001

Cited by 205 publications

(209 citation statements)

References 60 publications

(74 reference statements)

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“…This is unlikely to be the source of the heterogeneity reported here, because experiments were performed in 17-to 22-d-old rats, when the morphological maturation of the UBC brush is almost finished (Morin et al, 2001), and GABAergic transmission persists in adults (C.V.R., S.D., M.A.D., unpublished results). Moreover, at this time, mixed synapses are nearly mature in posterior structures (Keller et al, 2001;Awatramani et al, 2005). The developmental switch of mixed inhibition has been attributed to either postsynaptic (Keller et al, 2001;González-Forero and Alvarez, 2005) or presynaptic (Nabekura et al, 2004) mechanisms or to a reorganization of both specializations (Awatramani et al, 2005;Muller et al, 2006).…”

Section: Heterogeneity Of Inhibitory Synapses Onto Ubcsmentioning

confidence: 98%

“…In other structures, GABAergic inhibition may predominate in young synapses of mixed inhibitory networks, whereas glycinergic components prevail at more mature stages (Kotak et al, 1998;Keller et al, 2001;Nabekura et al, 2004). This is unlikely to be the source of the heterogeneity reported here, because experiments were performed in 17-to 22-d-old rats, when the morphological maturation of the UBC brush is almost finished (Morin et al, 2001), and GABAergic transmission persists in adults (C.V.R., S.D., M.A.D., unpublished results).…”

Section: Heterogeneity Of Inhibitory Synapses Onto Ubcsmentioning

confidence: 99%

“…Moreover, at this time, mixed synapses are nearly mature in posterior structures (Keller et al, 2001;Awatramani et al, 2005). The developmental switch of mixed inhibition has been attributed to either postsynaptic (Keller et al, 2001;González-Forero and Alvarez, 2005) or presynaptic (Nabekura et al, 2004) mechanisms or to a reorganization of both specializations (Awatramani et al, 2005;Muller et al, 2006). Presynaptic GoCs are characterized by different contents of GABA and glycine (Ottersen et al, 1988;Simat et al, 2007).…”

Section: Heterogeneity Of Inhibitory Synapses Onto Ubcsmentioning

confidence: 99%

“…The majority of inhibitory cells of the hindbrain express both neurotransmitters (Ottersen et al, 1988;Todd and Sullivan, 1990), which are accumulated in synaptic vesicles by the same transporter, VIAAT (vesicular inhibitory amino acid transporter) (McIntire et al, 1997;Sagné et al, 1997), and coreleased (Jonas et al, 1998;O'Brien and Berger, 1999). Mixed circuits undergo complex developmental maturation, whereby GABAergic inhibition dominates in young synapses whereas glycinergic inhibition appears later (Smith et al, 2000;Keller et al, 2001;Awatramani et al, 2005). This switch may be caused by either a presynaptic (Nabekura et al, 2004) or a postsynaptic (Keller et al, 2001;González-Forero and Alvarez, 2005) change.…”

Section: Introductionmentioning

confidence: 99%

“…Mixed circuits undergo complex developmental maturation, whereby GABAergic inhibition dominates in young synapses whereas glycinergic inhibition appears later (Smith et al, 2000;Keller et al, 2001;Awatramani et al, 2005). This switch may be caused by either a presynaptic (Nabekura et al, 2004) or a postsynaptic (Keller et al, 2001;González-Forero and Alvarez, 2005) change. Although maturation of both sides occurs (Awatramani et al, 2005;Muller et al, 2006), eventually creating mismatches (Dugué et al, 2005), corelease persists in the adult.…”

Section: Introductionmentioning

confidence: 99%

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Mixed Inhibitory Synaptic Balance Correlates with Glutamatergic Synaptic Phenotype in Cerebellar Unipolar Brush Cells

Rousseau¹,

Dugué²,

Dumoulin³

et al. 2012

J. Neurosci.

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Inhibitory synapses display a great diversity through varying combinations of presynaptic GABA and glycine release and postsynaptic expression of GABA and glycine receptor subtypes. We hypothesized that increased flexibility offered by this dual transmitter system might serve to tune the inhibitory phenotype to the properties of afferent excitatory synaptic inputs in individual cells. Vestibulocerebellar unipolar brush cells (UBC) receive a single glutamatergic synapse from a mossy fiber (MF), which makes them an ideal model to study excitatory-inhibitory interactions. We examined the functional phenotypes of mixed inhibitory synapses formed by Golgi interneurons onto UBCs in rat slices. We show that glycinergic IPSCs are present in all cells. An additional GABAergic component of large amplitude is only detected in a subpopulation of UBCs. This GABAergic phenotype is strictly anti-correlated with the expression of type II, but not type I, metabotropic glutamate receptors (mGluRs) at the MF synapse. Immunohistochemical stainings and agonist applications show that global UBC expression of glycine and GABA A receptors matches the pharmacological profile of IPSCs. Paired recordings of Golgi cells and UBCs confirm the postsynaptic origin of the inhibitory phenotype, including the slow kinetics of glycinergic components. These results strongly suggest the presence of a functional coregulation of excitatory and inhibitory phenotypes at the single-cell level. We propose that slow glycinergic IPSCs may provide an inhibitory tone, setting the gain of the MF to UBC relay, whereas large and fast GABAergic IPSCs may in addition control spike timing in mGluRII-negative UBCs.

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Section: Heterogeneity Of Inhibitory Synapses Onto Ubcsmentioning

confidence: 98%

Section: Heterogeneity Of Inhibitory Synapses Onto Ubcsmentioning

confidence: 99%

Section: Heterogeneity Of Inhibitory Synapses Onto Ubcsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Mixed Inhibitory Synaptic Balance Correlates with Glutamatergic Synaptic Phenotype in Cerebellar Unipolar Brush Cells

Rousseau¹,

Dugué²,

Dumoulin³

et al. 2012

J. Neurosci.

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Coronary angiography in the fully anticoagulated patient: The transradial route is successful and safe

Hildick‐Smith

Walsh

Löwe

et al. 2002

Cathet Cardio Intervent

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The radial approach to coronary angiography is intuitively attractive for fully anticoagulated patients (INR > 2) but no data exist concerning efficacy or safety of this procedure. The consensus view is that the femoral approach is contraindicated in fully anticoagulated patients, and though some operators undertake femoral catheterization in such patients and use closure devices, there are no data to suggest that it is safe to do so. At our institution, the radial approach for coronary angiography is reserved for patients in whom there is a relative contraindication to the femoral route. We have undertaken over 600 radial coronary angiograms in such patients since 1996, 66 of whom underwent transradial catheterization specifically because of anticoagulation status (INR > 2). Thirty-eight patients (58%) were male, average age 67 +/- 11 years. All 66 patients had an INR > 2 but < 4.5. The approach was left radial in 26 (39%), right radial in the remainder; sheath size was 4 Fr in 4 (6%), 5 Fr in 13 (20%), and 6 Fr in 49 (74%). Seven operators in total were involved, though two operators undertook the majority of cases (47; 71%). Success rate was 97%, with no failure of access, and only one minor postprocedural hemorrhage. Failures were due to radial artery atherosclerosis (1) and subclavian tortuosity (1). The radial approach to coronary angiography is safe and to be recommended in the fully anticoagulated patient.

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Glycine and GABA_A receptor subunits on Renshaw cells: Relationship with presynaptic neurotransmitters and postsynaptic gephyrin clusters

Geiman

Zheng

Fritschy

et al. 2002

J of Comparative Neurology

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Inhibitory synapses with large and gephyrin-rich postsynaptic receptor areas are likely indicative of higher synaptic strength. We investigated the presynaptic inhibitory neurotransmitter content (GABA, glycine, or both) and the presence and subunit composition of GABA(A) and glycine postsynaptic receptors in one example of gephyrin-rich synapses to determine neurochemical characteristics that could also contribute to enhance synaptic strength. Hence, we analyzed subunit receptor expression in gephyrin patches located on Renshaw cells, a type of spinal interneuron that receives powerful excitatory and inhibitory inputs and displays many large gephyrin patches on its surface. GABA(A) and glycine receptors were almost always colocalized inside Renshaw cell gephyrin clusters. According to the subunit-immunoreactivities detected, the composition of GABA(A) receptors was inferred to be either alpha(3)beta((2or3))gamma(2), alpha(5)beta((2or3))gamma(2), alpha(3)alpha(5)beta((2or3))gamma(2) or a combination of these. The types of neurotransmitters contained inside boutons presynaptic to Renshaw cell gephyrin patches were also investigated. The majority (60-75%) of terminals presynaptic to Renshaw cell gephyrin patches contained immunocytochemical markers for GABA as well as glycine, but a proportion contained markers only for glycine. Significantly, 40% of GABA(A) receptor clusters were opposed to presynaptic boutons that contained only glycinergic markers. We postulate that GABA and glycine corelease, and the presence of alpha3-containing GABA(A) receptors can enhance the postsynaptic current and contribute to strengthen inhibitory input on Renshaw cells. In addition, a certain degree of imprecision in the localization of postsynaptic GABA(A) receptors in regard to GABA release sites onto adult Renshaw cells was also found.

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Region-Specific Developmental Specialization of GABA–Glycine Cosynapses in Laminas I–II of the Rat Spinal Dorsal Horn

Cited by 205 publications

References 60 publications

Mixed Inhibitory Synaptic Balance Correlates with Glutamatergic Synaptic Phenotype in Cerebellar Unipolar Brush Cells

Mixed Inhibitory Synaptic Balance Correlates with Glutamatergic Synaptic Phenotype in Cerebellar Unipolar Brush Cells

Coronary angiography in the fully anticoagulated patient: The transradial route is successful and safe

Glycine and GABA_A receptor subunits on Renshaw cells: Relationship with presynaptic neurotransmitters and postsynaptic gephyrin clusters

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Region-Specific Developmental Specialization of GABA–Glycine Cosynapses in Laminas I–II of the Rat Spinal Dorsal Horn

Cited by 205 publications

References 60 publications

Mixed Inhibitory Synaptic Balance Correlates with Glutamatergic Synaptic Phenotype in Cerebellar Unipolar Brush Cells

Mixed Inhibitory Synaptic Balance Correlates with Glutamatergic Synaptic Phenotype in Cerebellar Unipolar Brush Cells

Coronary angiography in the fully anticoagulated patient: The transradial route is successful and safe

Glycine and GABAA receptor subunits on Renshaw cells: Relationship with presynaptic neurotransmitters and postsynaptic gephyrin clusters

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Product

Resources

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Glycine and GABA_A receptor subunits on Renshaw cells: Relationship with presynaptic neurotransmitters and postsynaptic gephyrin clusters