Region-specific gene expression in the epididymis

Belleannée, Clémence; Thimon, Véronique; Sullivan, Robert

doi:10.1007/s00441-012-1381-0

Cited by 123 publications

(115 citation statements)

References 163 publications

Supporting

Mentioning

115

Contrasting

Order By: Relevance

“…It is reported that the synthesis and secretion of epididymal proteins are regulated by temperature (Belleannee et al 2012), as well as by endocrine (Primiani et al 2007, Sipila et al 2011, paracrine (Turner et al 2007a, 2007b, Belleannee et al 2012 and lumicrine factors (Turner & Bomgardner 2002, Sipila et al 2011, Turner 2011, which are from the testis or from upstream regions of efferent ducts. Epididymal BPI may be regulated by these factors in addition to testosterone, which may explain the fact that Bpi expression was restored to 30% of its normal levels in orchidectomized mice treated with testosterone.…”

Section: Discussionmentioning

confidence: 99%

Bactericidal/permeability-increasing protein in the reproductive system of male mice may be involved in the sperm–oocyte fusion

Li¹,

Liu²,

Xia³

et al. 2013

Reproduction

View full text Add to dashboard Cite

Bactericidal/permeability-increasing protein (BPI) is a 455-residue (w55 kDa) protein found mainly in the primary (azurophilic) granules of human neutrophils. BPI is an endogenous antibiotic protein that belongs to the family of mammalian lipopolysaccharide (LPS)-binding and lipid transport proteins. Its major function is to kill Gram-negative bacteria, thereby protecting the host from infection. In addition, BPI can inhibit angiogenesis, suppress LPS-mediated platelet activation, increase DNA synthesis, and activate ERK/Akt signaling. In this study, we found that Bpi was expressed in the testis and epididymis but not in the seminal vesicles, prostate, and solidification glands. BPI expression in the epididymis increased upon upregulation of testosterone, caused by injection of GNRH. In orchidectomized mice, BPI expression was significantly reduced, but its expression was restored to 30% of control levels in orchidectomized mice that received supplementary testosterone. The number of sperm fused per egg significantly decreased after incubation with anti-BPI antiserum. These results suggest that BPI may take part in the process of sperm-oocyte fusion and play a unique and significant role in reproduction.

show abstract

Section: Discussionmentioning

confidence: 99%

Bactericidal/permeability-increasing protein in the reproductive system of male mice may be involved in the sperm–oocyte fusion

Li¹,

Liu²,

Xia³

et al. 2013

Reproduction

View full text Add to dashboard Cite

show abstract

“…Moreover, the specific regions of the epididymis (caput, corpus, cauda) possess distinct spatially restricted gene expression profiles, ensuring suitable epididymal functions essential to the different steps of sperm maturation, where factors such as steroidal hormones can affect the pattern of gene expression [83]. It has been confirmed that some genes encoding antioxidant enzymes are androgen-dependent [24,[30][31][32][33]35].…”

Section: Androgens As Regulators Of Antioxidant Enzymes In Epididymidesmentioning

confidence: 99%

Antioxidant enzyme expression of mRNA and protein in the epididymis of finasteride-treated male rat offspring during postnatal development

Kolasa¹,

Tarnowski²,

Baranowska-Bosiacka³

et al. 2019

aoms

View full text Add to dashboard Cite

A b s t r a c tIntroduction: We verify whether finasteride had a transgenerational effect on the epididymal expression of antioxidant enzymes, and the correlation between these enzymes and blood androgen concentrations in male offspring (F1:Fin) of females fertilized by finasteride-treated male rats. Material and methods: The expression of CAT, SOD1, GPX5, GR on the mRNA and protein levels was evaluated in the epididymis at postnatal day (PND) 7, 14, 21, 28 and 90. Levels of T and DHT were correlated with mRNA levels of enzymes by Spearman's rank correlation coefficient. Results: A change in the levels of transcripts was noted in F1:Fin rats: CAT decreased at PND 28 (p < 0.01) and increased at PND 90 (p < 0.01); SOD1 increased at PND 7 (p < 0.0001), 21 (p < 0.001), 90 (p < 0.0001) and decreased at 14 PND (p < 0.01); GPX5 increased at PND 14 and 21 (p < 0.0001); GR decreased at PND 21 and 28 (p < 0.0001). Altered immunolocalization of enzymes within the epididymal epithelium was observed. Negative correlations between GPX5 mRNA with androgens (T, p = 0.0002; DHT, p = 0.0009) were visible in the control rats, and positive correlation between DHT and CAT mRNA (p = 0.03), in opposite to F1:Fin group were was negative for both androgens (T, p = 0.044 and DHT, p = 0.02). Conclusions: Finasteride treatment of adult male rats may cause changes in antioxidant defense system in the epididymis of their offspring, leading to improper ROS concentrations that can affect post-testicular sperm maturation.

show abstract

“…Together with secretory activities of the tubular epithelium, these changes modulates metabolic pathways, such as second messenger and intracellular protein phosphorylation levels, energy metabolism, plasma membrane transport (Dacheux et al, 1989;Ecroyd et al, 2004;Jones et al, 2007;Saez et al, 2011;Voglmayr et al, 1985) (for review, refer to (Cornwall, 2009;Robaire et al, 2006)). This "passive" maturation is necessary because spermatozoa have no or very low capacity to synthesize new components, owing to their condensed DNA (Belleannee et al, 2012b).…”

Section: Role Of Epididymal Proteome In Sperm Maturationmentioning

confidence: 99%

The epididymis and its role on sperm quality and male fertility

Souza¹,

Schorr-Lenz²,

Lucca³

et al. 2017

Anim Reprod

View full text Add to dashboard Cite

The epididymis is a complex organ where spermatozoa acquire motility and ability to fertilize the egg. Epididymal maturation lasts 1 or 2 weeks and exposes the immature spermatozoa to a sequentially modified milieu, promoting intense interactions with secretions by the epididymal epithelium. Sperm surface modifications in response to interactions with epididymal secretions are key steps to achieve fertility ability. However, the precise molecular mechanisms that convert an immotile and infertile gamete into a highly motile cell capable of fusion with an oocyte are still unknown. Recent data on proteomics and transcriptomics of epididymal fluid and epididymosomes brought new ideas of the physiological roles of proteins and miRNAs in epididymal maturation in spermatozoa. This review focuses on the recent discoveries on epididymal fluid composition and its role on sperm maturation and preservation, linking to their survival and fertility potential.

show abstract

Region-specific gene expression in the epididymis

Cited by 123 publications

References 163 publications

Bactericidal/permeability-increasing protein in the reproductive system of male mice may be involved in the sperm–oocyte fusion

Bactericidal/permeability-increasing protein in the reproductive system of male mice may be involved in the sperm–oocyte fusion

Antioxidant enzyme expression of mRNA and protein in the epididymis of finasteride-treated male rat offspring during postnatal development

The epididymis and its role on sperm quality and male fertility

Contact Info

Product

Resources

About