Regional adaptations and parallel mutations in Feline panleukopenia virus strains from China revealed by nearly-full length genome analysis

Leal, Élcio; Liang, Ruiying; Liu, Qi; Villanova, Fabiola; Shi, Lisong; Liang, Lin; Li, Jinxiang; Cui, Shangjin

doi:10.1371/journal.pone.0227705

Cited by 7 publications

(5 citation statements)

References 39 publications

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“…The FPV sequences of lineages isolated from distinct hosts intermingle in phylogenetic trees, indicating that this virus easily spreads among distinct species. In the same way, the canine parvovirus (CPV) infects domestic dogs ( Canis lupus familiaris) , wolves ( Canis lupus) and coyotes ( Canis latrans ) [ 75 , 76 ]. Conversely, our phylogenetic analysis showed that evolutionary clades in CHPV are mostly structured according to the host in which this virus was identified.…”

Section: Discussionmentioning

confidence: 99%

Novel Chaphamaparvovirus in Insectivorous Molossus molossus Bats, from the Brazilian Amazon Region

Ramos

Abreu

Rodrigues

et al. 2023

Viruses

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Chaphamaparvovirus (CHPV) is a recently characterized genus of the Parvoviridae family whose members can infect different hosts, including bats, which constitute the second most diverse order of mammals and are described worldwide as important transmitters of zoonotic diseases. In this study, we identified a new CHPV in bat samples from the municipality of Santarém (Pará state, North Brazil). A total of 18 Molossus molossus bats were analyzed using viral metagenomics. In five animals, we identified CHPVs. These CHPV sequences presented the genome with a size ranging from 3797 to 4284 bp. Phylogenetic analysis-based nucleotide and amino acid sequences of the VP1 and NS1 regions showed that all CHPV sequences are monophyletic. They are also closely related to CHPV sequences previously identified in bats in southern and southeast Brazil. According to the International Committee on Taxonomy of Viruses (ICTV) classification criteria for this species (the CHPV NS1 gene region must have 85% identity to be classified in the same species), our sequences are likely a new specie within the genus Chaphamaparvovirus, since they have less than 80% identity with other CHPV described earlier in bats. We also make some phylogenetic considerations about the interaction between CHPV and their host. We suggest a high level of specificity of CPHV and its hosts. Thus, the findings contribute to improving information about the viral diversity of parvoviruses and show the importance of better investigating bats, considering that they harbor a variety of viruses that may favor zoonotic events.

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Section: Discussionmentioning

confidence: 99%

Novel Chaphamaparvovirus in Insectivorous Molossus molossus Bats, from the Brazilian Amazon Region

Ramos

Abreu

Rodrigues

et al. 2023

Viruses

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“…Parvoviruses can cross species barriers to infect less susceptible hosts using single or a few mutations [20]. Several amino acid residue changes in the threefold spike of VP2 could infuence antigenicity and host range [21,22]. Moreover, position 300 and adjacent residues at 299 and 301 in VP2 were separately involved in adapting CPV-2 binding to the canine transferrin receptor; a change in CPV-2VP2 residue 299 (Gly ⟶ Glu) caused the virus to lose its binding ability to canine TfR and 299-Glu severely restricts infection of CPV-2 in dog cells [23][24][25].…”

Section: Discussionmentioning

confidence: 99%

Identification of a Feline Panleukopenia Virus from Captive Giant Pandas (Ailuropoda melanoleuca) and Its Phylogenetic Analysis

Yang

Geng

Ouyang

et al. 2023

Transboundary and Emerging Diseases

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The host range of feline panleukopenia virus (FPV) is expanding and is a serious threat to both captive and free-range endangered wildlife. The FPV named FPV-am2020 was isolated from fecal samples from four diarrheal captive giant pandas in 2020, and pathogenicity and phylogenetic analysis were conducted in this study. Three-month-old cats challenged with FPV-am2020 experienced 100% mortality. The complete FPV-am2020 sequence was determined and comprised 5277 base pairs (bp), 36.76% GC content, and two open reading frames. According to the phylogenetic analysis of whole genome sequences and VP2 gene sequences, FPV-am2020 was closely related with MG764511.1 (isolated from captive lions in China, 2015), KX685354.1 (isolated from captive tigers in China, 2016), and KX900570.1 (isolated from captive jaguar in China, 1986). Furthermore, the study identified a G299E mutation in VP2 which was a key residue involved in phenotype changes in FPV. Thus, increased surveillance of FPV mutant isolates must be enacted to protect giant pandas against potential viral threats.

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“…These mutations can differentiate this virus from previously described CPPV-1 variants. Previous analysis of the ancestral reconstruction found that FPV can infect distinct carnivores with few changes in the VP2 gene [ 49 ]. Thus, the FPV-like parvovirus was tentatively named as a potentially novel virus detected in the fishing cats and this may support the first identification of this virus in this species.…”

Section: Discussionmentioning

confidence: 99%

Natural infection of parvovirus in wild fishing cats (Prionailurus viverrinus) reveals extant viral localization in kidneys

et al. 2021

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Carnivore protoparvovirus-1 (CPPV-1), a viral species containing feline panleukopenia virus (FPV) and canine parvovirus (CPV) variants, are widely spread among domestic and wild carnivores causing systemic fatal diseases. Wild fishing cats (Prionailurus viverrinus), a globally vulnerable species, have been found dead. Postmortem examination of the carcasses revealed lesions in intestine, spleen and kidney. CPPV-1 antigen identification in these tissues, using polymerase chain reaction (PCR) and immunohistochemistry (IHC), supported the infection by the virus. PCR- and IHC-positivity in kidney tissues revealed atypical localization of the virus while in situ hybridization (ISH) and transmission electron microscopy (TEM) with the pop-off technique confirmed the first description of viral localization in kidneys. Complete genome characterization and deduced amino acid analysis of the obtained CPPV-1 from the fishing cats revealed FPV as a causative agent. The detected FPV sequences showed amino acid mutations at I566M and M569R in the capsid protein. Phylogenetic and evolutionary analyses of complete coding genome sequences revealed that the fishing cat CPPV-1 genomes are genetically clustered to the FPV genomes isolated from domestic cats in Thailand. Since the 1970s, these genomes have also been shown to share a genetic evolution with Chinese FPV strains. This study is the first evidence of CPPV-1 infection in fishing cats and it is the first to show its localization in the kidneys. These findings support the multi-host range of this parvovirus and suggest fatal CPPV-1 infections may result in other vulnerable wild carnivores.

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Regional adaptations and parallel mutations in Feline panleukopenia virus strains from China revealed by nearly-full length genome analysis

Cited by 7 publications

References 39 publications

Novel Chaphamaparvovirus in Insectivorous Molossus molossus Bats, from the Brazilian Amazon Region

Novel Chaphamaparvovirus in Insectivorous Molossus molossus Bats, from the Brazilian Amazon Region

Identification of a Feline Panleukopenia Virus from Captive Giant Pandas (Ailuropoda melanoleuca) and Its Phylogenetic Analysis

Natural infection of parvovirus in wild fishing cats (Prionailurus viverrinus) reveals extant viral localization in kidneys

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