Regional Localization of 188 Sequence Tagged Sites on a Somatic Cell Hybrid Mapping Panel for Human Chromosome 3

Leach, Robin J.; Chinn, R.; Reus, Bonnie E.; Hayes, Shirley J.; Schantz, Laura J.; Dubois, Benjamin; Overhauser, Joan; Ballabio, Andrea; Drabkin, HA; Lewis, Tracey; Mendgen, G.; Naylor, Susan L.

doi:10.1006/geno.1994.1665

Cited by 18 publications

(11 citation statements)

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“…Our failure to isolate a D3S966 YAC might have been due to either a less efficient PCR of D3S966 than of D3S1448, or to an underrepresentation of the D3S966 YAC-containing yeast strain in the primary pools of the specific copy of the library we screened. By deletion hybrid mapping Leach et al (1995). recently showed that D3S1448 and D3S1449 (which we find to be identical, see below) map in an interval proximal to the interval containing D3S1298 and D3S1260 and distal to the interval containing D3S32.…”

Section: Discussionmentioning

confidence: 99%

Ordering of polymorphic markers in the chromosome region 3p21

Berg

Kooy²,

Hulsbeek³

et al. 1996

Cytogenet Genome Res

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Starting from five markers, with a well-defined order from distal to proximal 3p21 nine other markers could be inserted in this 3p21 map. Five were precisely mapped genetically. The other markers were ordered by FISH and/or deletion hybrid mapping. The overall 3p21 order from distal to proximal is as follows: D3S1298-D3S1260-(D3S966, D3S1448 (= D3S1449))-D3S1029-D3S32-D3S643-D3F15S2-D3S2968-D3S1235-D3S1289-D3S1447-D3S1295.

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Section: Discussionmentioning

confidence: 99%

Ordering of polymorphic markers in the chromosome region 3p21

Berg

Kooy²,

Hulsbeek³

et al. 1996

Cytogenet Genome Res

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“…Thus, rapid localizations o f very short sequences have been performed, and the linkage between the porcine genetic and cytogenetic maps has been increased. In the same way, this panel could also be used to map sequence tagged sites (Leach et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

A somatic cell hybrid panel for pig regional gene mapping characterized by molecular cytogenetics

Yerle

Echard

Robic

et al. 1996

Cytogenet Genome Res

279

179

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A panel of 27 pig × rodent somatic cell hybrids was produced and characterized cytogenetically. The first step of this study consisted of hybridizing a SINE probe to GTG-banded metaphases of each hybrid clone in order to count and identify the normal pig chromosomes and to detect rearranged ones. The second step consisted of using the DNA of each clone as a probe after pIRS-PCR (porcine interspersed repetitive sequence-polymerase chain reaction) amplification to highly enrich it in pig sequences. These probes, hybridized to normal pig metaphase chromosomes, enabled the identification of the complete porcine complement in the hybrid lines. Whole chromosomes and fragments were characterized quickly and precisely, and results were compared. In addition to this cytogenetic characterization, molecular verification was also carried out by using primers specific to six microsatellites and to one gene previously mapped to pig chromosomes. The results obtained allow us to conclude that we have produced a panel that is informative for all porcine chromosomes. This panel constitutes a highly efficient tool to establish not only assignments of genes and markers but also regional localizations on pig chromosomes.

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“…We further determined the location of KCNMB3 on chromosome 3 by specifically amplifying the first exon using primers P1 and P2 from a somatic cell hybrid panel of chromosome 3, which divides this chromosome into 23 intervals (8). PCR analysis of this panel resulted in the localization of exon 1 of KCNMB3 to 3q26.2-q27 (interval "T"), the region that is duplicated in the dup(3q) syndrome.…”

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confidence: 99%