Regional mapping of unique DNA sequences from human chromosome 3 derived from a flow-sorted chromosome library

Abstract

Eight single-copy DNA probes specific for human chromosome 3 were isolated by screening a human chromosome 3-derived genomic library. Southern blot analyses of DNAs isolated from a panel of somatic cell hybrids allowed us to regionally assign all probes to subregions on chromosome 3. Three clones were localized to the short arm of chromosome 3 (3p21→pter), two to the long arm (3q21→qter), and three to the 3q21→3p21 sub-region. Six of these DNA sequences map to regions overlapping a segment of chromosome 3 (3p14→p23) frequently deleted in small cell lung cancer cells. Restriction fragment length polymorphism analyses indicate that at least three of the eight single-copy probes studied show Msp1 or Bg/II polymorphisms. This library is a useful source of chromosome 3-specific probes.

Report of the committee on the genetic constitution of chromosome 3

“…Four of the probes (D3S42, D3S44, D3S45, and D3S46) are VNTR probes (Nakamura et al, 1988 H7281, H6674, H6675, H6676;respectively). Atchison et al (1988 have mapped 56 probes isolated from a flow sorted library (I.A03NS01) to chromosome 3. D3S35 and D3S39 are at 3p21, D3S36 is at 3q21, D3S37 is at 3q21-q22, D3S40 is at 3pl3-pl2, and D3S34 is at 3p21 -pi 4 by in situ hybridization.…”

High-resolution mapping of 10 unique DNA sequences to human chromosome 3 subregions by in situ hybridization

“…Each additional DNA sequence mapped to a site on the chromosome can provide a tool for mapping studies or for studies designed to identify disease-causing genes. The availability of human chromosome 3-specific genomic libraries (Deaven et al, 1986) facilitates isolation of DNA sequences derived from human chromosome 3 (Atchison et al, 1988(Atchison et al, , 1990(Atchison et al, . 1994bSmith et al, 1989).…”

Construction of gene libraries for each human chromosome

“…Characterization following are private communications unless otherwise referenced.Atchison ef a/(2). (k) Dietz-Band,(11).…”