Regional Molecular Genetic Key of Thirteen Snow Pool Aedes Species (Diptera: Culicidae) in Northern Colorado

West, D. F.; Payette, Terry; Mundy, Teri; Black, William C.

doi:10.1093/jmedent/34.4.404

Cited by 14 publications

(10 citation statements)

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“…This method was successfully used to construct the molecular key for Aedes species (West et al 1997). It was also used to distinguish closely related parasitic worms and others different organisms (Newton et al 1998, Wu et al 1999) and on studies of genetic variation and identification of snails of the genera Oncomelania, Bulinus and Biomphalaria (Hope & McManus 1994, Stothard et al 1996, Stothard & Rollinson 1997, Rollinson et al 1998, Vidigal et al 1998a,b, Caldeira et al 1998, Spatz et al 1998.…”

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confidence: 99%

Further studies on the molecular systematics of Biomphalaria snails from Brazil

Vidigal

Caldeira

Simpson³

et al. 2000

Mem. Inst. Oswaldo Cruz

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confidence: 99%

Further studies on the molecular systematics of Biomphalaria snails from Brazil

Vidigal

Caldeira

Simpson³

et al. 2000

Mem. Inst. Oswaldo Cruz

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“…key for Aedes species (West et aL, 1997). It was also used to distinguish closely related parasitic worms and other different organisms (Wu et aL, 1999).…”

Section: Using Restriction Fragment Length Polymorphisms Of Srdna Formentioning

confidence: 99%

RESTRICTION FRAGMENT LENGTH POLYMORPHISMS(RFLPs) OF THE SMALL-SUBUNIT RIBOSOMAL DNA AS A TOOL FOR IDENTIFICATION OF TILAPIA SPP.

El-Serafy

Awwad

Azab

et al. 2003

Egypt. J. of Aquatic Biolo. and Fish.

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I n Egypt, four species of Tilapia have been described based on morphometric, meristic and cytotaxonomical characteristics. These species are Tilapia zillii, Oreochromis niloticus, Oreochromis aureus and Sarotherodon galilaeus. The accurate identification of these fishes is complicated by the high variation in these characters, similarity among species and in some cases by the size of the fish. In this paper, we examined the use of polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLPs) analysis of the nuclear small subunit ribosomal RNA gene (srDNA) for molecular identification of Tilapia spp. in Egypt. The present study aims to evaluate such advanced molecular biological approach for identification of Tilapia spp. Genomic DNA was extracted from the four species of Tilapia. About 2000 bp 18S ribosomal DNA was amplified by PCR using specific primers. The technique of restriction fragment length polymorphisms was used to identify the specific 18S rDNA for each species. O. niloticus rDNA 'RFLPs species-specific pattern is proved by digestion with restriction endonucleases AIwNI and Aval, On the other hand, digestion of amplified 18S rDNA with endonuclease enzymes Smal, Xmal and Sstll produced speciesspecific patterns for T. zillii, O. aureus and S. galilaeus respectively. This indicates the efficiencies of these restriction endonuclease enzymes in species-specific identification of Tilapia spp. Restriction fragment length polymorphisms of the nuclear srDNA (RFLPs\PCR) proved to be a good tool for checking the relationships among species and their subspecies, even more than the morphological analysis. 466Sabry S. Ei-Serafr et ai.

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“…It involves using the 18S rDNA of such organisms by means of PCR amplification and digestion with different restriction enzymes. This method was successfully employed to construct the molecular key for Aedes species West et al, (1997). It was also used to distinguish closely related parasitic worms and other different organisms (Wu et al, 1999) and.…”

Section: Introductionmentioning

confidence: 99%