Regional variation in composition and antimicrobial activity of US propolis against Paenibacillus larvae and Ascosphaera apis

Wilson, Michael B.; Brinkman, Doug; Spivak, Marla; Gardner, Gary; Cohen, Jerry D.

doi:10.1016/j.jip.2014.10.005

Cited by 73 publications

(92 citation statements)

References 23 publications

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“…Four of the 12 samples were poor inhibitors of P. larvae growth, but good inhibitors of A. apis growth, suggesting that resin availability in these climatic regions was deficient in terms of providing broad-spectrum antibiotic activity (Wilson et al 2015). Thus, regional differences in floral vegetation (resin donors) can very strongly determine antibiotic activity with highly variable therapeutic potential.…”

Section: Resin and Propolismentioning

confidence: 99%

Pharmacophagy and pharmacophory: mechanisms of self-medication and disease prevention in the honeybee colony (Apis mellifera)

2015

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-Apitherapy promises cures for diseases in human folk medicine, but the effects of honeybee produced and foraged compounds on bee health are less known. Yet, hive products should chiefly facilitate medication and sanitation of the honeybees themselves rather than other organisms. We here review the impact of both self-produced gland secretions and foraged hive products (pharmacognosy) on colony health. Although foraged plant-derived compounds vary highly in antibiotic activity depending on the floral and regional origins, secondary plant metabolites in honey, pollen and propolis are important for the antibiotic activity against pathogens and parasites. However, specific bee health-enhancing activities of bee products should clearly be distinguished from the effects of an intact nutrition ensuring the basic immune competence of bees. Further unravelling the interactions among groups of active substances or individual compounds used in concert with specific behavioural adaptations will deepen our understanding of the natural potential of honeybees to maintain colony health.honey / propolis / pollen / bee bread / royal jelly / antimicrobial activity / self-medication / host-parasite interaction

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Section: Resin and Propolismentioning

confidence: 99%

Pharmacophagy and pharmacophory: mechanisms of self-medication and disease prevention in the honeybee colony (Apis mellifera)

2015

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“…Paenibacillus larvae (from stock strains obtained from the USDA Agricultural Research Service culture collection, NRRL no. B-2605) were cultured in brain/heart infusion broth and the bacterial growth assay was conducted following the methods of Wilson et al (2015). Propolis extracts were diluted in acetonitrile to final concentrations ranging from 175 to 8 mg l…”

Section: Seasonal Antimicrobial Activity Of Propolismentioning

confidence: 99%

“…Numerous in vitro studies have demonstrated the inhibitory activity of propolis, and specific compounds within propolis, against the growth of the honey bee bacterial pathogen Paenibacillus larvae and Ascosphaera apis (Antúnez et al, 2008;Bastos et al, 2008;Bilikova et al, 2013;Lindenfelser, 1968;Wilson et al, 2013Wilson et al, , 2015. It is not known whether honey bees actually consume propolis, but Johnson et al (2012) demonstrated that when bees were experimentally fed propolis in sucrose syrup, the transcription of three cytochrome 450s, involved in pesticide detoxification, was induced (Johnson et al, 2006;Mao et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Seasonal benefits of a natural propolis envelope to honey bee immunity and colony health

Borba

Klyczek

Mogen

et al. 2015

Journal of Experimental Biology

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Honey bees, as social insects, rely on collective behavioral defenses that produce a colony-level immune phenotype, or social immunity, which in turn impacts the immune response of individuals. One behavioral defense is the collection and deposition of antimicrobial plant resins, or propolis, in the nest. We tested the effect of a naturally constructed propolis envelope within standard beekeeping equipment on the pathogen and parasite load of large field colonies, and on immune system activity, virus and storage protein levels of individual bees over the course of a year. The main effect of the propolis envelope was a decreased and more uniform baseline expression of immune genes in bees during summer and autumn months each year, compared with the immune activity in bees with no propolis envelope in the colony. The most important function of the propolis envelope may be to modulate costly immune system activity. As no differences were found in levels of bacteria, pathogens and parasites between the treatment groups, the propolis envelope may act directly on the immune system, reducing the bees' need to activate the physiologically costly production of humoral immune responses. Colonies with a natural propolis envelope had increased colony strength and vitellogenin levels after surviving the winter in one of the two years of the study, despite the fact that the biological activity of the propolis diminished over the winter. A natural propolis envelope acts as an important antimicrobial layer enshrouding the colony, benefiting individual immunity and ultimately colony health.

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“…Few works have studied the role played by these resins in the control and resistance to the diseases in honey bee colonies. Propolis has proven to be active against the main bee threats such as the bacterium Paenibacillus larvae (Antúnez et al, 2008;Bastos et al, 2008;Mihai et al, 2012), the fungus Ascosphaera apis (Wilson et al, 2015), and the mite Varroa destructor (Damiani et al, 2010). In colonies of Apis mellifera L. (Hymenoptera: Apidae), the propolis added into the nest environment modified the expression of immunerelated genes in the exposed worker bees (Simone, Evans, & Spivak, 2009).…”

Section: Introductionmentioning

confidence: 99%

Effect of Propolis Oral Intake on Physiological Condition of Young Worker Honey Bees, Apis Mellifera L.

Damiáni

Porrini

Lancia

et al. 2017

Journal of Apicultural Science

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A b s t r a c t Honey bees collect resin from various plant species and transform it into propolis that is incorporated into the nest. The role of resins in the bee health field is poorly understood. The aim was to evaluate the effects of forced consumption of propolis on the physiological condition and short-term survival of Apis mellifera worker bees. It was tested if the number of circulating hemocytes in hemolymph, the abdominal fat bodies and the hypopharyngeal glands development were affected by the feeding with propolis extracts in laboratory conditions during the warm and the cold seasons. Propolis added to sugar candy was consumed by workers for fourteen days without affecting the bee survival. The number of circulating hemocytes in hemolymph remained constant despite the differential diet during the experiment. However, the development of fat bodies and hypopharyngeal glands was altered by propolis ingestion. The abdominal fat body development in winter bees diminished after fourteen days of propolis consumption, while it increased in summer bees. The hypopharyngeal gland development decreased for the assayed period in workers from both seasons. Our results encourage us to continue exploring this research field and learn how long-term forced ingestion of a plant-derived compound, a non-nutritive substance, can modify physiological bee parameters. A broader understanding of the multiple roles of propolis in the health of the honey bee colonies could be obtained by studying the ways in which it is processed and metabolized and the effect that generates in another physiological responses.

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Regional variation in composition and antimicrobial activity of US propolis against Paenibacillus larvae and Ascosphaera apis

Cited by 73 publications

References 23 publications

Pharmacophagy and pharmacophory: mechanisms of self-medication and disease prevention in the honeybee colony (Apis mellifera)

Pharmacophagy and pharmacophory: mechanisms of self-medication and disease prevention in the honeybee colony (Apis mellifera)

Seasonal benefits of a natural propolis envelope to honey bee immunity and colony health

Effect of Propolis Oral Intake on Physiological Condition of Young Worker Honey Bees, Apis Mellifera L.

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