Regioselective Photolabeling of Glycophorin A in Membranes

Glycophorin A requirement for expression of O‐linked antigens on the erythrocyte membrane

“…Erythrocytes were suspended in Hank's balanced salt solution containing 2% foetal calf serum and 10 m m 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulphonic acid (HEPES) with appropriate dilutions of the TER‐119 antibody (Kina et al . 2000) or M1/69 (Springer et al . 1978), and followed by secondary staining with fluorescein isothiocyanate (FITC)‐conjugated mouse anti‐rat kappa‐chain antibody (Lanier et al .…”

Synthesis and characterization of photolabeling probes of miltefosine

“…This result indicates that probe 1 still retains the amphiphilic nature of miltefosine. Similar findings has been reported on the modification of phospholipid probes designed to investigate the lipid-water interfaces of biomembranes [16].…”

“…In probe 2, the photoactivatable group is located in the alkyl chain, which was designed to probe the interactions in which miltesoine is involved in the hydrophobic membrane core. The molecular design on which these probes were based was inspired by previous studies on photolabling probes designed for investigating the effects of phospholipids in biomemebranes and membrane proteins, where photoactivatable groups were introduced either into the polar head or into the fatty acid chain of the phospholipids [15,16]. Here, we report on the synthesis and characterization of the two photolabeling probes designed for studying the biological effects of miltefosine.…”

Photolabeling Strategies to Study Membranotropic Peptides Interacting with Lipids and Proteins in Membranes

“…They observed that most of the cross-linking involved the carboxyl group of Glu 70 , thus suggesting that this residue was located within the bilayer . However, 20 years later the Nakatani group used the same approach with a different lipid probe and obtained very different results. , They used a benzophenone-based bola-phospholipid probe ( 10 , Figure ), thus guaranteeing that the label was located at the center of the bilayer, with no possibility for looping back. DSC characterization of the probe 10 showed complete miscibility with DMPC in the fluid phase .…”

“…23 However, 20 years later the Nakatani group used the same approach with a different lipid probe and obtained very different results. 24,25 They used a benzophenone-based bola-phospholipid probe (10, Figure 2), thus guaranteeing that the label was located at the center of the bilayer, with no possibility for looping back. DSC characterization of the probe 10 showed complete miscibility with DMPC in the fluid phase.…”