Registration of BTx623<sub>ms8</sub>, a New and Easily Identifiable Nuclear Male Sterile Mutant in Sorghum

Xin, Zhanguo; Chen, Junping; Jiao, Yinping; Gladman, Nicholas; Hayes, Chad; Burow, Gloria; Emendack, Yves; Burke, John J.

doi:10.3198/jpr2017.09.0063crgs

Cited by 6 publications

(7 citation statements)

References 15 publications

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“…The dw5 mutant was backcrossed to the male sterile NIL of BTx623, BTx623 ms8 (Xin et al, 2018) and the backcrossed F 1 and F 2 populations were used to examine the genetic control of dwarf phenotype in dw5 mutant. The results of plant height segregation are provided in Table 1, and the plant height measurement data are provided in Table 2.…”

Section: Methodsmentioning

confidence: 99%

“…he dwarf phenotype of the mutant plants was conirmed in M 5 progenies and F 2 segregation population under ield condition at the USDA-ARS Cropping Systems Research Laboratory in Lubbock, TX (33.58° N, 101.85° W, elevation 976 m). In 2017, the seed of original wild-type BTx623, the male sterile near-isogenic line (NIL) of BTx623 (BTx623 ms8 ) (Xin et al, 2018) used as female for backcrossing, the dw5, and backcrossed F 1s were planted in the winter nursery ield in Guayanilla, Puerto Rico (18.0373° N, 66.7963° W, elevation 49 m), on 11 Dec. 2017. he plot size for all plantings at both Lubbock and Puerto Rico locations was 4.6 m long with 1.02 m row spacing. About 60 sorghum seeds per row were planted per plot, and the planting depth was about 3 cm.…”

Section: Methodsmentioning

confidence: 99%

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Registration of BTx623_dw5, a New Sorghum Dwarf Mutant

Chen

Xin

Laza

2019

J of Plant Registrations

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The USDA-ARS has released a new dwarf sorghum [Sorghum bicolor (L.) Moench) mutant BTx623 dw5 (Reg. No. GS-787, PI 688506). Dwarf genes have been an important driving force in breeding since the Green Revolution. Single dwarf locus is used to breed high-yielding semidwarf wheat (Triticum aestivum L.) and rice (Oryza sativa L.) cultivars. However, in sorghum, any of the known dwarf loci alone are insuicient to breed semidwarf grain sorghum itting for modern farm practices. Therefore, four dwarf loci have been traditionally used collectively in combinations to breed sorghum cultivars of the desired plant height for machine harvesting. Here we register a new sorghum dwarf mutant isolated from a mutagenized BTx623 mutant library, which is genetically diferent from those currently known controlling the dwarf phenotype in sorghum. Therefore, we designated this newly identiied dwarf genotype as dwarf 5 (dw5). The dwarf phenotype of dw5 was evaluated in two environments: the Puerto Rico winter nursery with short days and mild temperature conditions and the Lubbock, TX, summer ield with long days and high temperature conditions. The dw5 mutation reduced the plant height of BTx623 from 155 to 74 cm in Puerto Rico and from 113 to 72 cm in Lubbock. The dwarf phenotype in dw5 mutant is caused by a single nuclear gene mutation and is inherited in a recessive manner. It can be easily identiied and bred into other sorghum lines through recurrent backcrossing. The dw5 mutation provides a new way to control sorghum height.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Registration of BTx623_dw5, a New Sorghum Dwarf Mutant

Chen

Xin

Laza

2019

J of Plant Registrations

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“…Pollen from the bagged bmr mutants was crossed to BTx623ms8, a near-isogenic line of BTx623 with a nuclear male sterile mutation (Xin et al, 2017(Xin et al, , 2018. Due to the large number of the mutants selected at the M 2 generation that displayed poor seed setting, systematic evaluation of mutant phenotypes was deferred to the M 3 generation.…”

Section: Parental Background and Germplasm Developmentmentioning

confidence: 99%

Registration of three new bmr12 sorghum mutants from an ethyl methane sulfonate–induced BTx623 mutant population

Emendack

Xin

Hayes

et al. 2022

J of Plant Registrations

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Three new sorghum (Sorghum bicolor L.) brown midrib (bmr) genetic stocks BTx623bmr12-30 (Reg. no. GS-790, PI 699404), PI 699405), PI 699406) were developed for release by USDA-ARS in Lubbock, TX. The primary purpose for the release of these stocks is to provide an extensive source of (bmr12) variants for use in forage breeding programs. The genetic stocks were isolated from the pedigreed sorghum ethyl methane sulfonate-mutagenized sorghum in BTx623 background. The derived mutations were analyzed by DNA sequencing and confirmed by KASP (Kompetitive Allele Specific PCR) markers. The confirmed bmr mutants were also analyzed for stem and leaf lignin and carbohydrate contents. Furthermore, physiological and agro-morphological evaluations were performed under control and field conditions.All genetic stocks displayed the reddish-brown pigmentation in the leaf midrib, confirming the bmr phenotype. The bmr12 mutants flowered 4-5 d earlier than the wild type BTx623. Physical and compositional characterization of seeds from the three genetic stocks revealed no negative consequence of bmr on grain structure. Notably, these bmr12 mutants had 14-24% greater total seed protein than wild type BTx623.The three new bmr12 alleles provide public and private breeders crucial and novel genetic stock sources for use in breeding bmr forage sorghum.

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“…We then performed complementation analyses to determine whether ms9 is allelic to any previously reported sorghum NMS mutants (Pedersen and Toy, 2001;Xin et al, 2018). Phenotyping results revealed that all cpF1 plants from the complementation crosses between male-sterile plants of known NMS lines and heterozygous BC2F1 ms9 plants were fertile (Table 1).…”

Section: Ms9 Is a Novel Recessive Nuclear Male Sterility Mutantmentioning

confidence: 99%

Identification of the First Nuclear Male Sterility Gene (Male‐sterile 9) in Sorghum

Chen¹,

Jiao

Laza³

et al. 2019

The Plant Genome

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The Plant Genome S orghum is a major grain crop used for human consumption and animal feed as well as a promising bioenergy crop for sugar, biomass, and biofuel production (de Siqueira Ferreira et al., 2013; Rooney et al., 2007). Like maize (Zea mays L.), sorghum is a diploid C4 plant that, in contrast to maize, is well adapted to droughtprone and high-temperature environments and can thrive on marginal soils (Morris et al., 2013). Sorghum also has a much smaller genome than maize (~800 vs. 2500 Mb), and after the recent completion of a highquality diploid genome sequence, sorghum has become an emerging model for highly productive C4 crops (McCormick et al., 2018; Paterson et al., 2009). Heterosis, or hybrid vigor, the ability of hybrids to outperform the best inbred line parents is probably the most important strategy to increase grain yield in many crops including sorghum (Kim and Zhang, 2018). A critical requirement of using hybrids to increase yield is the ability to produce a pure male-sterile female parent that

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Registration of BTx623_ms8, a New and Easily Identifiable Nuclear Male Sterile Mutant in Sorghum

Cited by 6 publications

References 15 publications

Registration of BTx623_dw5, a New Sorghum Dwarf Mutant

Registration of BTx623_dw5, a New Sorghum Dwarf Mutant

Registration of three new bmr12 sorghum mutants from an ethyl methane sulfonate–induced BTx623 mutant population

Identification of the First Nuclear Male Sterility Gene (Male‐sterile 9) in Sorghum

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Registration of BTx623ms8, a New and Easily Identifiable Nuclear Male Sterile Mutant in Sorghum

Cited by 6 publications

References 15 publications

Registration of BTx623dw5, a New Sorghum Dwarf Mutant

Registration of BTx623dw5, a New Sorghum Dwarf Mutant

Registration of three new bmr12 sorghum mutants from an ethyl methane sulfonate–induced BTx623 mutant population

Identification of the First Nuclear Male Sterility Gene (Male‐sterile 9) in Sorghum

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Registration of BTx623_ms8, a New and Easily Identifiable Nuclear Male Sterile Mutant in Sorghum

Registration of BTx623_dw5, a New Sorghum Dwarf Mutant

Registration of BTx623_dw5, a New Sorghum Dwarf Mutant