Registration of Maize Germplasm Line GEMS‐0067

Campbell, Mark; Jane, Jay‐lin; Pollak, Linda M.; Blanco, Mike; O'Brien, Anna

doi:10.3198/jpr2006.10.0640crg

Cited by 37 publications

(31 citation statements)

References 4 publications

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“…GUAT209:S13 is a breeding cross from the Germplasm Enhancement of Maize (GEM) project of USDA (Pollak 2002) and likely served as high amylose modifier gene(s) source that, together with the recessive amylose extender (ae) gene, raises the starch amylose percentage to at least 70%. To our knowledge GEMS-0067 represents the only public source of a maize inbred line of high amylose content to date in the US (Campbell et al 2007). Other studies were conducted with this material including ethanol production, starch thermal properties, and measurements by near infrared transmittance spectroscopy (Campbell et al 1999;Polaske et al 2005;Wu et al 2006).…”

Section: Introductionmentioning

confidence: 98%

Genetic analysis of high amylose content in maize (Zea mays L.) using a triploid endosperm model

Campbell

Yen

et al. 2008

Euphytica

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Traditionally, high amylose starch (HAS) from maize (Zea mays L.) has been mainly used as an ingredient in gum candies and as an adhesive for corrugated cardboard. Two recent advances have increased interest in the use of HAS. The first one has been the development of starch-based biodegradable thermo plastics. Second, high amylose maize is a source of resistant starch (RS), a type of starch that resists digestion. As a food additive, consumers can benefit from added RS since it will lower the glycemic index and the risk of colon cancer in accordance with recent research in food science. Normal maize has about 25% amylose starch. A maize inbred line, GEMS-0067 (Reg. no GP-550, PI 643420) possesses high amylose modifier gene(s) that, together with the recessive amylose extender (ae) gene, raises the starch amylose percentage to at least 70%. The objective of this study was to determine the gene effects, nonallelic interactions and heritability of high amylose content in maize using Bogyo's triploid model. Nine populations were derived from a cross between H99ae, a maize inbred line with 55% amylose starch, and GEMS-0067. Data were collected from two locations in Missouri (MO) and South Dakota (SD) over 2 years (2005 and 2006). Incomplete dominance explained some of the inheritance of HAS. Maternal effects were also detected. The triploid models for MO and SD were separately established based on the corresponding data in 2005 and 2006. The additive and type 1 dominance effects in MO, and the additive, type 1 dominance, type 2 dominance, and additive 9 additive in SD were significantly different from zero meaning that those effects played an important role in amylose synthesis. Both broad-sense and narrowsense heritabilities were high indicating that high amylose content could be effectively selected for in a segregating population.

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Section: Introductionmentioning

confidence: 98%

Genetic analysis of high amylose content in maize (Zea mays L.) using a triploid endosperm model

Campbell

Yen

et al. 2008

Euphytica

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“…5% or unique starch thermal properties), sources of disease and insect resistance, reduced mycotoxins and yield (Pollak, 2003;Blanco et al, 2005). Recently, a source of germplasm with modifier genes that enhance amylose content above 70% was released (Campbell et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the grain methionine, lysine and tryptophan contents of maize ( Zea mays L.) germplasm in the Germplasm Enhancement of Maize Project

Scott

Blanco

2009

Plant Genet. Resour.

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The Germplasm Enhancement of Maize (GEM) Project is a cooperative effort between the USDA-ARS, private industry and public researchers to broaden and enhance the germplasm base of maize. In this program, selected accessions from the Latin American Maize Project, and seven tropical hybrids donated by DeKalb to the GEM Project, were crossed to elite proprietary inbred lines contributed by commercial plant breeding programs. In most cases, the resulting hybrids were crossed to a second commercial inbred line and the resulting 25% exotic hybrids were used as breeding populations for further development. To identify GEM germplasm with value to protein quality breeding programs, we developed a process for evaluating the content of the essential amino acids methionine, lysine and tryptophan in the grain of GEM germplasm that balances the need for multiple-year evaluations with the constantly changing entry list of this germplasm screening program. This process involves annual field trials with common checks. Weak entries are dropped from the trial each year to make room for new entries, while strong entries are retained. Methionine exhibited the most significant variation, followed by lysine and then tryptophan. A number of GEM lines had methionine or lysine levels that were significantly better than Corn Belt checks and some were competitive with high-amino acid checks.

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“…The GUAT ae parent is the first public 70% amylose line (Campbell et al 2007). For easier discussion and comparison, the progeny crosses are separated into four groups and identified by the first parent, creating the mutant groupings of dull sugary2 (du su2), amylose-extender sugary2(ae su2), amylose-extender dull (ae du), and an amylose-extender with introgressed Guatemalan germplasm (GUAT ae).…”

Section: Corn Materialsmentioning

confidence: 99%

Exotic Corn Lines with Increased Resistant Starch and Impact on Starch Thermal Characteristics

2010

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Ten parent corn lines, including four mutants (dull sugary2, amyloseextender sugary2, amylose-extender dull, and an amylose-extender with introgressed Guatemalen germplasm [GUAT ae]) and six lines with introgressed exotic germplasm backgrounds, were crossed with each other to create 20 progeny crosses to increase resistant starch (RS) as a dietary fiber in corn starch and to provide materials for thermal evaluation. The resistant starch 2 (RS2) values from the 10 parent lines were 18.3-52.2% and the values from the 20 progeny crosses were 16.6-34.0%. The %RS2 of parents was not additive in the offspring but greater RS2 in parents was correlated to greater RS2 in the progeny crosses (r = 0.63). Differential scanning calorimetry (DSC) measured starch thermal characteristics, revealing positive correlations of peak gelatinization temperature and change in enthalpy with %RS2 (r = 0.65 and r = 0.67, P ≤ 0.05); however, % retrogradation (a measure of RS3) and retrogradation parameters did not correlate with %RS2. The %RS2 and onset temperature increased with the addition of the ae gene, likely because RS delays gelatinization. RightsWorks produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. Ten parent corn lines, including four mutants (dull sugary2, amyloseextender sugary2, amylose-extender dull, and an amylose-extender with introgressed Guatemalen germplasm [GUAT ae]) and six lines with introgressed exotic germplasm backgrounds, were crossed with each other to create 20 progeny crosses to increase resistant starch (RS) as a dietary fiber in corn starch and to provide materials for thermal evaluation. The resistant starch 2 (RS2) values from the 10 parent lines were 18.3-52.2% and the values from the 20 progeny crosses were 16.6-34.0%. The %RS2 ABSTRACT

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Registration of Maize Germplasm Line GEMS‐0067

Cited by 37 publications

References 4 publications

Genetic analysis of high amylose content in maize (Zea mays L.) using a triploid endosperm model

Genetic analysis of high amylose content in maize (Zea mays L.) using a triploid endosperm model

Evaluation of the grain methionine, lysine and tryptophan contents of maize ( Zea mays L.) germplasm in the Germplasm Enhancement of Maize Project

Exotic Corn Lines with Increased Resistant Starch and Impact on Starch Thermal Characteristics

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