Registration of ‘Morales’ Small White Bean

Abstract

Registration of 'Rosada Nativa' Pink Bean 'RosadaNativa' pink bean (Phaseolus vulgaris L.) (Reg. no. CV-164, PI 606250) was developed and released in 1998 by the Puerto Rico Agricultural Experiment Station in cooperation with the USD A-ARS. RosadaNativa, tested as PR9443-1, was derived from the cross DOR483/BelNeb Rust Resistant-1. DOR483 is a bean golden mosaic (BGMV) resistant small red breeding line from CI AT. BelNeb Rust Resistant-1 (2) is a great northern bean cultivar that has resistance to bean rust [caused by Uromyces appendiculatus (Pers.:Pers.) Unger] and common bacterial blight [caused by Xanthomonas axonopodis pv. phaseoli Starr & Garces 1950 emend. Vauterin et al. 1995; syn. X. campestris pv. phaseoli (Smith) Dye]. Crosses, field selections, and evaluations were made at the Isabela Substation in Puerto Rico. The F t was advanced in the field. Individual F 2 and F 3 plants were selected under field conditions for erect plant architecture, early maturity, and commercially acceptable pink seed type. The F 4 and F 5 were evaluated for plant type, relative maturity and rust resistance. The best plants from selected families were harvested in bulk. The F 6 was screened for BGMV resistance using a greenhouse inoculation technique (1). The BGMV resistance of the RosadaNativa was confirmed in field trials conducted in Puerto Rico and the Dominican Republic. Rosada Nativa has the recessive bgm-I allele for resistance to BGMV. The presence of bgm-1, a recessive resistance gene, was confirmed using the R2 57 o/s3o RAPD marker (3). Rosada Nativa represents the first release of a pink bean with BGMV resistance. Rosada Nativa is resistant to the bean rust races prevalent in Puerto Rico and the Dominican Republic. It also carries the / gene for resistance to BCMV. Rosada Nativa is moderately resistant to web blight (caused by Rhizoctonia solani Kiihn) and susceptible to common bacterial blight.

Achievements and limitations of contemporary common bean breeding using conventional and molecular approaches

“…Singh et al (2007) Mahuku et al (2004) Angular leaf spot and anthracnose resistance Singh et al (2003a) Anthracnose resistance Co-1, Co-2 (black bean) Kelly et al (2001) Co-1, Co-2 (dark red kidney bean) Kelly et al (1998b) Co-6 (small red bean) Young and Kelly (1996) C0-4 2 (Pinto bean) Miklas et al (2003) Bean common mosaic and bean common necrotic mosaic resistance I, bc3 (black bean) Kelly et al (1994) I, bc3 (kidney bean) I, bc-1 2 (great northern) Stewart-Williams et al (2003) bc-1 2 (small red) Hosfield et al (2004) bc-1 2 (pinto) Brick et al (2001) and Grafton et al (1999) Bean golden yellow mosaic resistance Pyramided genes producing high levels of resistance Singh et al (2000), Beaver and Miklas (1999), and Rosas et al (1997) High levels of resistance derived from scarlet runner bean (Phaeolus coccineus l.) Beaver et al (2005) Bruchid resistance Osborn et al (2003) Common bacterial blight High levels of resistance derived from tepary bean (Phaseolus acutifolius L.) Singh et al (2001b) High levels of resistance derived from the scarlet runner bean (Phaseolus coccineus L.) Zapata et al (2004) and Miklas et al (1999) Drought tolerance Brick et al (2008), Beebe et al (2008), and Singh et al (2001a) Halo blight resistance Coyne et al (2000) Heat tolerance Beaver et al (2008), Rosas et al (2003a), and Rosas et al (2000b) Heat tolerance derived from tepary beans Rainey and Griffiths (2004).…”

Registration of Tepary Germplasm with Multiple‐Stress Tolerance, TARS‐Tep 22 and TARS‐Tep 32

“…Subsequently, greenhouse grown plants in 15‐cm‐diameter pots were inoculated with the multiple needle technique (Andrus, 1948; Zapata et al, 1985). The experiment was evaluated in a randomized complete block design with four replications using resistant line VAX 6 and the susceptible cultivar ‘Morales’ (PI 606249; Beaver and Miklas, 1999) as controls. The CBB reactions were evaluated at 14 d post‐inoculation on a scale of 1 to 9, where 1 = no symptoms and 9 = systemic infection causing complete infection of the leaf (van Schoonhoven and Pastor‐Corrales, 1987).…”

Registration of PR0633-10 and PR0737-1 Red Mottled Dry Bean Germplasm Lines with Resistance to BGYMV, BCMV, BCMNV, and Common Bacterial Blight

“…Plants were inoculated with BCMNV approximately 1 wk after emergence (Morales, 1989). The cultivar Morales with the I gene for BCMV resistance was used as a susceptible control (Beaver and Miklas, 1999). Because plants that develop a lethal hypersensitive systemic necrosis reaction to BCMNV strains NL3 or NL8 are known to carry the dominant I allele, these plants were considered to have that allele by virtue of being resistant to BCMV (Haley et al, 1994).…”