Regression of diet-induced atherosclerosis in Güttingen Miniature Swine

Kobari, Yukage; Koto, Masao; Tanigawa, Manabu

doi:10.1258/002367791781082478

Cited by 14 publications

(10 citation statements)

References 21 publications

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“…24 During the next 9 months of regression on a conventional diet, serum lipid levels returned to baseline and there was regression of fatty streaks but not of fibrous plaques. 24 The induction of atherosclerosis for 8 months, followed by a regression period of 4 months that included feeding of fish oil, did not induce the regression of atherosclerosis but prevented the progression of established lesions. 25 In a later study, 26 the effect of fish oil was examined in minipigs fed the atherogenic diet for 8 months followed by a normal diet alone or with fish oil.…”

Section: Swinementioning

confidence: 97%

Does Therapeutic Intervention Achieve Slowing of Progression or Bona Fide Regression of Atherosclerotic Lesions?

Stein

2001

ATVB

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Abstract-This review focuses on the regression of atherosclerosis in humans and experimental animals. It highlights the difficulties to determine unequivocally whether with a given therapeutic intervention, such as diet, drugs, or apheresis, the progression of lesions was curtailed or bona fide regression of atherosclerotic lesions was achieved. It seems appropriate to mention that 2 very different ways to measure regression were used in experimental animals and in humans. Regression in animals was determined mainly in the aorta or coronary arteries isolated at post mortem, and the criteria used were degree of sudanophilia and/or aortic wall thickness and cellular composition or cholesterol content. In humans, the evaluation of regression relied mainly on quantitative coronary angiography. The literature of the past decade is reviewed selectively but not exhaustively, and in some instances, a brief historical overview is given.

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Section: Swinementioning

confidence: 97%

Does Therapeutic Intervention Achieve Slowing of Progression or Bona Fide Regression of Atherosclerotic Lesions?

Stein

2001

ATVB

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“…There are 2 main types of dietary protocol, one containing cholesterol and animal lipids [5]–[9], and the other containing cholesterol, animal lipids, and sucrose [10], [11]. Some studies have focused their attention on certain kinds of candidate genes with specific functions, but this has not clarified a complete projection of whole blood RNA profiles of the transitions caused by diet-induced hyperlipidemia.…”

Section: Discussionmentioning

confidence: 99%

“…High-fat, high-cholesterol, and high-sugar diets have been shown to induce hyperlipidemia, obesity, and insulin resistance in humans and rodents [2]–[4]. Dietary-induced hyperlipidemia pig models have also been established [5]–[11].…”

Section: Introductionmentioning

confidence: 99%

Oligonucleotide Microarray Analysis of Dietary-Induced Hyperlipidemia Gene Expression Profiles in Miniature Pigs

et al. 2012

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BackgroundHyperlipidemia animal models have been established, but complete gene expression profiles of the transition from normal lipid levels have not been obtained. Miniature pigs are useful model animals for gene expression studies on dietary-induced hyperlipidemia because they have a similar anatomy and digestive physiology to humans, and blood samples can be obtained from them repeatedly.MethodologyTwo typical dietary treatments were used for dietary-induced hyperlipidemia models, by using specific pathogen-free (SPF) Clawn miniature pigs. One was a high-fat and high-cholesterol diet (HFCD) and the other was a high-fat, high-cholesterol, and high-sucrose diet (HFCSD). Microarray analyses were conducted from whole blood samples during the dietary period and from white blood cells at the end of the dietary period to evaluate the transition of expression profiles of the two dietary models.Principal FindingsVariations in whole blood gene expression intensity within the HFCD or the HFCSD group were in the same range as the controls provide with normal diet at all periods. This indicates uniformity of dietary-induced hyperlipidemia for our dietary protocols. Gene ontology- (GO) based functional analyses revealed that characteristics of the common changes between HFCD and HFCSD were involved in inflammatory responses and reproduction. The correlation coefficient between whole blood and white blood cell expression profiles at 27 weeks with the HFCSD diet was significantly lower than that of the control and HFCD diet groups. This may be due to the effects of RNA originating from the tissues and/or organs.ConclusionsNo statistically significant differences in fasting plasma lipids and glucose levels between the HFCD and HFCSD groups were observed. However, blood RNA analyses revealed different characteristics corresponding to the dietary protocols. In this study, whole blood RNA analyses proved to be a useful tool to evaluate transitions in dietary-induced hyperlipidemia gene expression profiles in miniature pigs.

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“…High-fat, high-cholesterol, and high-sugar diets have been shown to induce hyperlipidemia, obesity, and insulin resistance in humans and rodents [20][21][22]. Dietary-induced hyperlipidemia pig models have also been established [23][24][25][26][27][28][29].…”

Section: Gene Expression Profiles Change Related To Hyperlipidemiamentioning

confidence: 99%

Whole Blood RNA Analysis, Aging and Disease

Takahashi¹,

Takatsu²,

Misawa³

et al. 2012

Blood Cell - An Overview of Studies in Hematology

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Regression of diet-induced atherosclerosis in Güttingen Miniature Swine

Cited by 14 publications

References 21 publications

Does Therapeutic Intervention Achieve Slowing of Progression or Bona Fide Regression of Atherosclerotic Lesions?

Does Therapeutic Intervention Achieve Slowing of Progression or Bona Fide Regression of Atherosclerotic Lesions?

Oligonucleotide Microarray Analysis of Dietary-Induced Hyperlipidemia Gene Expression Profiles in Miniature Pigs

Whole Blood RNA Analysis, Aging and Disease

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