Reguladores vegetais no enraizamento de estacas lenhosas da amoreira-preta cv. Xavante

Maia, Aline José; Botelho, Renato Vasconcelos

doi:10.5433/1679-0359.2008v29n2p323

Cited by 18 publications

(15 citation statements)

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“…However, the value of correlation coefficient for the relationship between the percentage of shoots that actually formed root and increasing concentration of IBA was R = 0.871, suggesting a negative relationship between the application of increasing concentrations of IBA and rooting, at least up to certain level (Figure 2). The relationship observed between auxin level and rooting potential has been recorded for other plants where high levels of auxin may have inhibitory effect over the rooting process (Pedroti and Voltolini, 2001;Erig and Schuch, 2004;Henrique et al, 2006;Maia and Botelho, 2008). Following this, the regenerated plants were acclimatized and transferred to the green house ( Figure 1F).…”

Section: Rootingsupporting

confidence: 54%

Analysis of organogenic competence of cotyledons of Jatropha curcas and their in vitro histological behavior

Nogueira¹,

Soares²,

Ibrahim³

et al. 2011

Afr. J. Biotechnol.

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Using cut pieces cotyledons from germinating zygotic embryos of Jatropha curcas, we monitored the series of anatomical events leading to the generation of shoot through organogenesis by histological analysis. 14 days old cotyledons that were pre-cultured in a half strength Murashige and Skoog (MS) media supplemented with 100 mg/L myoinositol and 10 mg/L thiamine HCl, were cultured in organogenic competence induction media (CIM) comprising of MS salts with 1.5 mg/L benzyl adenine (BA) and 0.05 mg/L indole-3-butyric acid (IBA) and incubated for the induction of organogenic competence. Following their sequential transfer to shoot induction media (containing MS + 1.5 mg/L BA, 0.05 mg/L IBA and 0.5 mg/L GA 3 ); shoot elongation media (containing MS + 0.3 mg/L BA) and rooting media (containing half strength MS + IBA at different concentrations), we selected individual explants and subjected them to histological analysis in order to study the morphological changes occurring during organogenesis. Our findings show that to induce organogenesis using cut pieces of cotyledons, these tissues must be harvested at least by the 14th day following germination. Optimal organogenic competence was attained after 21 days incubation period in the dark where most of the explants showed evidence of protruding shoots surrounded by calli with various morphological features. Up to 53.91% of the total explants cultured in this study produced well defined shoots with each explant producing an average number of 1.5 shoots bearing two to eight leaves. We recorded the highest percentage of root formation, which stood at 27.50% when the shoots were cultured in a rooting media containing 0.3 mg/L IBA. Histological analysis of the different events occurring during the process of organogenesis suggest that the protuberances arising from parenchymatous cells and perhaps bundle sheath forming meristematic centres acquiring organogenic competence have a multicellular origin, indicating that the regeneration process takes place through direct organogenesis.

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Section: Rootingsupporting

confidence: 54%

Analysis of organogenic competence of cotyledons of Jatropha curcas and their in vitro histological behavior

Nogueira¹,

Soares²,

Ibrahim³

et al. 2011

Afr. J. Biotechnol.

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“…O fato da 'Brazos' ter apresentado maior porcentagem de estacas enraizadas sem aplicação de AIB pode indicar um conteúdo endógeno de auxinas apropriado para o processo de formação de raízes. MAIA & BOTELHO (2008), avaliando o enraizamento de estacas da cultivar 'Xavante', relataram haver necessidade de aplicação de AIB, pois observaram 0% de enraizamento nas estacas sem utilização de AIB. Possivelmente, esses autores utilizaram estacas sem folhas, pois, de acordo com os dados do presente trabalho, pode-se observar que a porcentagem de enraizamento é bastante superior quando se utiliza estacas com folhas.…”

Section: Resultsunclassified

Presença de folhas no enraizamento de estacas de amoreira-preta

et al. 2014

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“…Dentre as auxinas sintéticas mais utilizadas no processo de estaquia, o ácido indolbutírico (AIB) é bastante efetivo para um grande número de espécies e relativamente estável, sendo pouco suscetível à ação dos sistemas de enzimas de degradação de auxinas, além de não ser tóxico à maioria das plantas (PIRES & BIASI, 2003). No entanto, o efeito benéfico das auxinas exógenas varia entre espécies, cultivares e concentrações (VILLA et al, 2003;MAIA & BOTELHO, 2008;LONE et al, 2010b).…”

Section: Introductionunclassified