Regulated Cell Death in Pulpitis

Huang, Minchun; Zhan, Chang’an A.; Xiao-jun, Yang; Hou, Jin

doi:10.1016/j.joen.2020.07.006

Cited by 10 publications

(5 citation statements)

References 105 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consequently, there exists an urgent need to explore effective solutions to address the issues. Relevant studies show that aerobic and anaerobic bacteria in the pericoronal blind bag are the main pathogens for reverse pulpitis [ 12 – 14 ]. Therefore, effective measures are entailed to eliminate the bacteria in the peripheral bag, which can be realized by local medication to reach the lesion, kill the bacteria, and eliminate pathogenic microorganisms.…”

Section: Discussionmentioning

confidence: 99%

Minocycline plus Zinc Oxide Eugenol Cement Might Be A Promising Alternative for Acute Pulpitis

Song

Lei

Liu

2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Objective. To investigate the clinical effect of minocycline plus zinc oxide eugenol cement in the treatment of acute pulpitis and its effect on the levels of HIF-1α, Bcl-2, and tumor necrosis factor α. Methods. A total of 286 patients with acute pulpitis who were treated in our hospital from January 2019 to October 2020 were recruited and assigned (1 : 1) via random number table method to receive either minocycline (control group) or minocycline plus zinc oxide eugenol cement (study group). Outcome measures included treatment effect, tooth mobility, tooth percussion pain score, hypoxia-inducible factor (HIF)-1α and B-lymphocyte tumor (Bcl)-2 positive rate, and tumor necrosis factor (TNF-α). Results. Minocycline plus zinc oxide eugenol cement was associated with significantly lower scores of teeth mobility and percussion pain versus minocycline alone ( p < 0.05 ). Minocycline plus zinc oxide eugenol cement resulted in a significantly higher treatment efficacy (97.20%) versus minocycline alone (72.73%) ( p < 0.05 ). Minocycline plus zinc oxide eugenol cement was associated with significantly lower positive rates of HIF-1α and Bcl-2 and lower levels of TNF-α versus minocycline alone ( p < 0.05 ). The patients receiving minocycline plus zinc oxide eugenol cement showed significantly lower visual analogue scale (VAS) scores and faster pain relief versus those given minocycline alone ( p < 0.05 ). Conclusion. Minocycline plus zinc oxide eugenol cement offers a viable alternative for acute pulpitis as it mitigates the pain of patients, alleviates inflammatory responses, and lowers the positive rate of HIF-1α and Bcl-2, so it is worthy of clinical promotion.

show abstract

Section: Discussionmentioning

confidence: 99%

Minocycline plus Zinc Oxide Eugenol Cement Might Be A Promising Alternative for Acute Pulpitis

Song

Lei

Liu

2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…Activated inflammatory caspases such as caspase‐1 can cleave gasdermin D (GSDMD) to generate an N‐terminal p30 fragment (GSDMD‐N) that forms plasma membrane pores, leading to pyroptosis (He et al, 2015). Recent studies have demonstrated that intrinsic and extrinsic apoptosis and/or pyroptosis may constitute critical strategies for maintaining homeostasis in pulpitis, periodontitis and osteoarthritis (An et al, 2020; Huang et al, 2020; Rocha et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Enterococcus faecalis OG1RF induces apoptosis in MG63 cells via caspase‐3/‐8/‐9 without activation of caspase‐1/GSDMD

Wen

Zhong

et al. 2021

Oral Diseases

View full text Add to dashboard Cite

Objective Regulated cell death is key in the pathogenesis of persistent apical periodontitis. Here, we investigated the mechanisms of regulated cell death in osteoblast‐like MG63 cells infected with Enterococcus faecalis OG1RF. Materials and methods MG63 cells were infected with live E. faecalis OG1RF at the indicated multiplicity of infection for the indicated infection time. We evaluated the cells by flow cytometry, terminal deoxynucleotidyl transferase dUTP nick end labelling assay and lactate dehydrogenase release analysis; measured the activity of caspase‐1/‐3/‐8/‐9 and the release of interleukin‐1β; and determined the expression of apoptosis‐associated proteins and gasdermin D by apoptosis antibody array and Western blotting. Results Enterococcus faecalis OG1RF reduced the mitochondrial membrane potential of the infected cells, increased the percentage of apoptotic and terminal deoxynucleotidyl transferase dUTP nick end labelling‐positive cells, and enhanced lactate dehydrogenase release. The expression of caspase‐3 and survivin and the activity of caspase‐3/‐8/‐9 were upregulated, while the expression of death receptor 6 was downregulated. The activity of caspase‐1/gasdermin D and the release of interleukin‐1β remained unaltered. Conclusion Enterococcus faecalis OG1RF induced both intrinsic and extrinsic MG63 cell apoptosis via caspase‐3/‐8/‐9 activation but did not activate the pyroptotic pathway regulated by caspase‐1/gasdermin D.

show abstract

“…Pulpitis is a common disease that leads to non-vital teeth, as well as teeth missing (Huang et al, 2020). Although dental procedures, such as root canal treatment, might partially rescue tooth function, issues such as splitting, poor biological perception, uneven occlusal stress, and food impaction can remain.…”

Section: Introductionmentioning

confidence: 99%

Pleiotrophin attenuates the senescence of dental pulp stem cells

et al. 2021

View full text Add to dashboard Cite

Objectives: Pleiotrophin (PTN), a secreted extracellular matrix-associated protein, plays an important role in regulating the osteo/dentinogenic differentiation potential of dental pulp stem cells (DPSCs). Our previous study has demonstrated that PTN expression in young DPSCs was is 10-fold higher than that in aged DPSCs. However, the role of PTN on the in maintaining the stemness of senescent DPSCs remains unclear. The present study aimed to investigate the effect of PTN on senescent DPSCs in vitro. Materials and Methods: Dental pulp stem cells were isolated from human third molars. PTN was knocked down using short hairpin RNAs to study the role of PTN on the senescence of DPSCs. DPSCs with aging performance were obtained by a replicative senescence cell model was obtained by the long-term culture of DPSCs to the 15th passage in vitro (P15). We then investigated the effect of PTN on senescent DPSCs (P15 DPSCs). Real-time RT-PCR, western blotting, alizarin red staining, quantitative calcium analysis, SAβ-Gal staining, CFSE, and cell-counting kit-8 (CCK8) assays were used to study cellular senescence and function. Results:The depletion of PTN increased the ratio of SAβ-gal-positive cells, upregulated the expression of p16, and down-regulated the expression of TERT and p-p38. Furthermore, 50 pg/ml of PTN recombinant protein rescued these changes the altered ratio of SAβ-gal-positive cells, decreased the expression of p16, enhanced TERT and p-p38 expression, as well as telomere activity, caused by PTN depletion and long-term culture. The15th passage cells displayed typical aging characteristic, including high ratio of SAβ-gal-positive cells, increased aging-related gene expression, decreased proliferation rate, high level of Cyclin D expression, and impaired osteo/ dentinogenic differentiation potential. However, 50 pg/ml of PTN recombinant protein could partially reverse these alteration rescue these changes. Conclusions:The present study demonstrated that PTN could protect DPSCs from senescence by improving the proliferation and osteo/dentinogenic differentiation ability, probably through the p38 MAPK pathway.

show abstract

Regulated Cell Death in Pulpitis

Cited by 10 publications

References 105 publications

Minocycline plus Zinc Oxide Eugenol Cement Might Be A Promising Alternative for Acute Pulpitis

Minocycline plus Zinc Oxide Eugenol Cement Might Be A Promising Alternative for Acute Pulpitis

Enterococcus faecalis OG1RF induces apoptosis in MG63 cells via caspase‐3/‐8/‐9 without activation of caspase‐1/GSDMD

Pleiotrophin attenuates the senescence of dental pulp stem cells

Contact Info

Product

Resources

About