Regulated expression of glutamyl‐tRNA synthetase is directed by a mobile genetic element in the cyanobacterium <i>Tolypothrix</i> sp. PCC 7601

Luque, Ignacio; Andújar, Alfonso; Jia, Lin; Zabulon, Gérald; Marsac, Nicole Tandeau de; Flores, Enrique; Houmard, Jean

doi:10.1111/j.1365-2958.2006.05170.x

Cited by 15 publications

(11 citation statements)

References 47 publications

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“…Although IS elements are typically associated with genetic rearrangements, other functions have been reported, including service as promoter elements for nearby genes (24). There are, however, no reports of the gene regulation in Francisella observed in the present study occurring on a global scale (23,24,30). Moreover, this is the first report of polyamines regulating transcription from IS elements.…”

Section: Discussioncontrasting

confidence: 42%

Global Transcriptional Response to Spermine, a Component of the Intramacrophage Environment, Reveals Regulation of Francisella Gene Expression through Insertion Sequence Elements

Carlson¹,

Horzempa²,

O’Dee³

et al. 2009

J Bacteriol

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Tularemia is caused by the category A biodefense agent Francisella tularensis. This bacterium is associated with diverse environments and a plethora of arthropod and mammalian hosts. How F. tularensis adapts to these different conditions, particularly the eukaryotic intracellular environment in which it replicates, is poorly understood. Here, we demonstrate that the polyamines spermine and spermidine are environmental signals that alter bacterial stimulation of host cells. Genomewide analysis showed that F. tularensis LVS undergoes considerable changes in gene expression in response to spermine. Unexpectedly, analysis of gene expression showed that multiple members of two classes of Francisella insertion sequence (IS) elements, ISFtu1 and ISFtu2, and the genes adjacent to these elements were induced by spermine. Spermine was sufficient to activate transcription of these IS elements and of nearby genes in broth culture and in macrophages. Importantly, the virulent strain of F. tularensis, Schu S4, exhibited similar phenotypes of cytokine induction and gene regulation in response to spermine. Distinctions in gene expression changes between Schu S4 and LVS at one orthologous locus, however, correlated with differences in IS element location. Our results indicate that spermine and spermidine are novel triggers to alert F. tularensis of its eukaryotic host environment. The results reported here also identify an unexpected mechanism of gene regulation controlled by a spermine-responsive promoter contained within IS elements. Different arrangements of these mobile genetic elements among Francisella strains may contribute to virulence by conveying new expression patterns for genes from different strains.

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Section: Discussioncontrasting

confidence: 42%

Global Transcriptional Response to Spermine, a Component of the Intramacrophage Environment, Reveals Regulation of Francisella Gene Expression through Insertion Sequence Elements

Carlson¹,

Horzempa²,

O’Dee³

et al. 2009

J Bacteriol

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“…Moreover, as one of aminoacyl-tRNA synthetases, glutamyl-tRNA synthetase can participate in a wide variety of biological processes including RNA processing and trafficking, apoptosis, transcription of rRNA, angiogenesis, and inflammation (Lee et al 2004;Park et al 2005). Recently, structure and regulated expression of glutamyltRNA synthetase were further presented (Levican et al 2005;Luque et al 2006;Schulze et al 2006). Although a large number of studies on glutamyl-tRNA synthetase were reported, almost all these studies focused on microorganisms and animals and knowledge of plant glutamyl-tRNA synthetase remains quite limited.…”

Section: Discussionmentioning

confidence: 99%

Identification and fine mapping of a thermo-sensitive chlorophyll deficient mutant in rice (Oryza sativa L.)

Liu

et al. 2007

Planta

117

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A thermo-sensitive chlorophyll deficient mutant was isolated from more than 15,000 transgenic rice lines. The mutant displayed normal phenotype at 23 degrees C or lower temperature (permissive temperature). However, when grown at 26 degrees C or higher (nonpermissive temperature) the plant exhibited an abnormal phenotype characterized by yellow green leaves. Genetic analysis revealed that a single nuclear-encoded recessive gene is responsible for the mutation, which is tentatively designed as cde1(t) (chlorophyll deficient 1, temporally). PCR analysis and hygromycin resistance assay indicated the mutation was not caused by T-DNA insertion. To isolate the cde1(t) gene, a map-based cloning strategy was employed and 15 new markers (five SSR and ten InDels markers) were developed. A high-resolution physical map of the chromosomal region around the cde1(t) gene was made using F(2) and F(3) population consisting of 1,858 mutant individuals. Finally, the cde1(t) gene was mapped in 7.5 kb region between marker ID10 and marker ID11 on chromosome 2. Sequence analysis revealed only one candidate gene, OsGluRS, in the 7.5 kb region. Cloning and sequencing of the target region from the cde1(t) mutant showed that a missense mutation occurred in the mutant. So the OsGluRS gene (TIGR locus Os02 g02860) which encode glutamyl-tRNA synthetase was identified as the Cde1(t) gene.

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“…Western blots were performed as described previously (29). Antibodies against an internal peptide (EYDRKKDESPSVEAC) of Anabaena ValRS were obtained from rabbits and affinity purified (Genscript, Piscataway, NJ).…”

Section: Methodsmentioning

confidence: 99%

Membrane Anchoring of Aminoacyl-tRNA Synthetases by Convergent Acquisition of a Novel Protein Domain

Olmedo‐Verd

Santamaría-Gómez

Alda

et al. 2011

Journal of Biological Chemistry

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Background: Aminoacyl-tRNA synthetases (aaRS) are enzymes that couple tRNAs to their cognate amino acids. Results: aaRSs from cyanobacteria containing the novel CAAD protein domain are localized in the thylakoid membrane. Conclusion: Confinement of aaRSs to the membrane is advantageous for cyanobacteria under specific conditions. Significance: These findings provide the first description of membrane-bound aaRSs.

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Regulated expression of glutamyl‐tRNA synthetase is directed by a mobile genetic element in the cyanobacterium Tolypothrix sp. PCC 7601

Cited by 15 publications

References 47 publications

Global Transcriptional Response to Spermine, a Component of the Intramacrophage Environment, Reveals Regulation of Francisella Gene Expression through Insertion Sequence Elements

Global Transcriptional Response to Spermine, a Component of the Intramacrophage Environment, Reveals Regulation of Francisella Gene Expression through Insertion Sequence Elements

Identification and fine mapping of a thermo-sensitive chlorophyll deficient mutant in rice (Oryza sativa L.)

Membrane Anchoring of Aminoacyl-tRNA Synthetases by Convergent Acquisition of a Novel Protein Domain

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