Regulating the acrosome reaction

Florman, Harvey M.; Jungnickel, Melissa K.; Sutton, Keith A.

doi:10.1387/ijdb.082696hf

Cited by 143 publications

(145 citation statements)

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“…The propagation of a Ca 2+ -induced wave produced from the opening of Ca 2+ channels along the flagella is a necessary milestone in sperm maturation and makes hyperactivated motility possible [4,54,55]. Additionally, [Ca 2+ ] i plays a pivotal role in acrosome exocytosis [56][57][58][59][60]. Specific ion channels mediate the rise in [Ca 2+ ] i within sperm, and many candidates for the principle Ca 2+ channel in sperm, including various voltagegated Ca 2+ channels, were proposed based primarily on studies using Ca 2+ imaging, immunolabeling, and patch-clamp experiments from sperm precursors [61][62][63].…”

Section: Calcium Channels and Hyperactivationmentioning

confidence: 99%

Flagellar ion channels of sperm: similarities and differences between species

et al. 2015

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a b s t r a c tMotility and fertilization potential of mammalian sperm are regulated by ion homeostasis which in turn is under tight control of ion channels and transporters. Sperm intracellular pH, membrane voltage and calcium concentration ([Ca 2+ ] i ) are all important for sperm activity within the female reproductive tract. While all mammalian sperm are united in their goal to find and fertilize an egg, the molecular mechanisms they utilize for this purpose are diverse and differ between species especially on the level of ion channels. Recent direct recording from sperm cells of different species indicate the differences between rodent, non-human primate, ruminant, and human sperm on the basic levels of their ion channel regulation. In this review we summarize the current knowledge about ion channel diversity of the animal kingdom and concentrate our attention on flagellar ion channels of mammalian sperm.

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Section: Calcium Channels and Hyperactivationmentioning

confidence: 99%

Flagellar ion channels of sperm: similarities and differences between species

et al. 2015

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“…The physiologically relevant AR changes in [Ca 2+ ] i include external and internal Ca 2+ sources (Breitbart et al 2010 ;Costello et al 2009 ;Darszon et al 2011 ;Florman et al 2008 ). At the present time three different Ca 2+ channels are thought to mediate the [Ca 2+ ] i responses associated to the AR.…”

Section: The Acrosome Reactionmentioning

confidence: 94%

“…At the present time three different Ca 2+ channels are thought to mediate the [Ca 2+ ] i responses associated to the AR. They appear to be functionally linked in a manner that is not fully understood Florman et al 2008 ;Publicover et al 2007 ). Early on it was thought that voltage-dependent Ca 2+ (Ca V ) channels were involved in the initial [Ca 2+ ] i increase detected during the AR induced by ZP in mouse sperm, taking into account their pharmacology and that of this reaction .…”

Section: The Acrosome Reactionmentioning

confidence: 99%

“…Evidence indicates internal Ca 2+ stores (i.e., the acrosome) participate releasing Ca 2+ through the IP3 receptor, the second type of Ca 2+ channel involved in the AR, which is activated as a consequence of IP3 production during the AR Florman et al 2008 ;Mayorga et al 2007 ;Publicover et al 2007 (Costello et al 2009 ;Darszon et al 2012 ).…”

Section: The Acrosome Reactionmentioning

confidence: 99%

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Cl− Channels and Transporters in Sperm Physiology

Treviño

Orta

Figueiras-Fierro

et al. 2014

Sexual Reproduction in Animals and Plants

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Spermatozoa must decode environmental and cellular cues to succeed in fertilization, and this process relies heavily on ion channels. New observations bring to light the relevant participation of Cl − channels and anion transporters in some of the main sperm functions. Here we review the evidence that indicates the participation of Cl − channels in motility, maturation, and the acrosome reaction (AR), and what is known about their molecular identity and regulation. Our better understanding of sperm anion transport will yield tools to handle some infertility problems, improve animal breeding and preserve biodiversity, and develop selective and secure male contraceptives.

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“…12 Acrosome Reaction (AR) This acrosome reaction is an exocytic secretory event within the sperm head that releases proteolytic enzymes for zona pellucida penetration and remodels the sperm surface in preparation for oocyte fusion. 1,13 It is triggered by sperm-zona binding. The AR status of sperm is assessed using fluorescent lectins that bind to the acrosomal membrane or to the acrosomal contents.…”

Section: Sperm Morphologymentioning

confidence: 99%

An Assessment of New Sperm Tests for Male Infertility

Natali

Turek

2011

Urology

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The routine semen analysis, although used for more than 50 years, fails to accurately distinguish between fertile and infertile men. As a consequence, many tests of sperm function (TSF) have been developed. This review discusses both older and newer diagnostic TSF. It outlines the principles underlying each assay and reviews aggregate clinical data to determine its current relevance and utility. It concludes that the relevance of many older TSF is questionable, with the wide acceptance of intracytoplasmic sperm injection (ICSI). Newer TSF have the potential to deliver more clinically relevant information but require more extensive study to better understand their predictive role in the ICSI era. UROLOGY 77: 1027-1034, 2011. © 2011 Elsevier Inc. All rights reserved.I n addition to the medical history and physical examination, the conventional semen analysis has been an essential laboratory test for the evaluation of male fertility for at least 50 years. However, the idea that fertility can be defined by threshold values of semen parameters is a concept that is fundamentally flawed. 1 Although not a true measure of fertility, the semen analysis, if abnormal, suggests that the probability of achieving fertility is lower than normal. 2 Because of biological variability, two semen analyses are generally needed, performed with two to three days of sexual abstinence and evaluated in a standardized fashion. 2 Recognized normal values for semen parameters are given in Table 1 in agreement with the latest recommendations by the World Health Organization (WHO). 2 Traditionally, (except for the latest WHO recommendations), these references are derived by expert consensus and not by prospective clinical trials and thus their true relationship to male fertility is unclear. In addition, the definition of what constitutes "normal" semen parameters is constantly challenged. A meta-analysis of 29 US studies of semen quality from 9612 fertile, or presumably fertile, men suggested that a sperm concentration of 98 million/mL is normal. 3 Although sperm motility is considered the "best" predictor of fertility, normal sperm motility ranges from 53% to 62%. 4 Thus, simply deriving "normal" semen parameters has been a prohibitively lengthy and inconclusive process to date.Finally, other biological variables affect the clinical utility of the semen analysis. In addition to wide intraindividual variation, seasonal 5 and geographic variation 4 further complicate the performance of the semen analysis as a fertility measure. As examples, within-subject, interejaculate coefficients of variation for sperm concentration and motility are estimated at 44.7% and 15% in one study. 1 Thus, even with excellent quality control, wide biological variation in semen quality profoundly challenges the notion that the conventional semen analysis can accurately assess male fertility. OLDER ADJUNCTIVE SPERM TESTSBecause of the need to more precisely characterize normal fertile semen, adjunctive semen testing has become popular (Figure 1). The concept behind de...

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Regulating the acrosome reaction

Cited by 143 publications

References 101 publications

Flagellar ion channels of sperm: similarities and differences between species

Flagellar ion channels of sperm: similarities and differences between species

Cl− Channels and Transporters in Sperm Physiology

An Assessment of New Sperm Tests for Male Infertility

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