2012
DOI: 10.1016/j.bbrc.2012.02.082
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Regulation and spatial organization of PCNA in Trypanosoma brucei

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Cited by 25 publications
(30 citation statements)
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“…The previous study followed TbPCNA expression in a single parasite and reported irregular patterns of TbPCNA expression in M phase parasites. 23 Such irregular expression patterns in M phase parasites might explain why TbPCNA was not detected in the nucleus of a single parasite. We examined multiple parasites per field of view and detected TbPCNA expression in the cytoplasm and nucleus of M phase parasites from endogenous and inducible TbPCNA clones.…”
Section: Discussionmentioning
confidence: 99%
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“…The previous study followed TbPCNA expression in a single parasite and reported irregular patterns of TbPCNA expression in M phase parasites. 23 Such irregular expression patterns in M phase parasites might explain why TbPCNA was not detected in the nucleus of a single parasite. We examined multiple parasites per field of view and detected TbPCNA expression in the cytoplasm and nucleus of M phase parasites from endogenous and inducible TbPCNA clones.…”
Section: Discussionmentioning
confidence: 99%
“…22 A recent study used TbPCNA to establish the temporal and spatial patterns of DNA replication in T. brucei and reported differential regulation of TbPCNA during the cell cycle. 23 We examined the consequences that deregulating intra-parasite TbPCNA levels had on proliferation and DNA replication in bloodstream form T. brucei. This study demonstrates that either depleting or overexpressing TbPCNA severely reduces proliferation and DNA replication in the parasite.…”
Section: Introductionmentioning
confidence: 99%
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“…It should be noted that regulation of DNA replication is a complex process and requires the interplay of many replication factors at the origins. Indeed, homologs of TLK, Asf1, and PCNA have been identified and are found essential for DNA replication in trypanosomes (56,64). Undoubtedly, more replication factors await our further exploration, and more work needs to be done to integrate these factors into existing pathways.…”
Section: Dna Replication Licensing and Regulation Of S-phase Progressionmentioning
confidence: 99%
“…Basic knowledge about PCNA/PIP-box interactions has translated to the pre-clinical proof-of-mechanism for the inhibitor T2AA to therapeutically target HsPCNA in cancer cells (Actis et al, 2013;Inoue et al, 2014;Punchihewa et al, 2012;Tan et al, 2012). There is a homolog of PCNA in T. brucei, TbPCNA, which is regulated through the cell cycle, and which is located at the nuclear periphery (Kaufmann et al, 2012). Currently, no information is available about mechanism and interactions of TbPCNA, with no associated proteins have been identified to date that interact with the PCNA of T. brucei (TbPCNA).…”
Section: Introductionmentioning
confidence: 99%