Regulation of a multipotent stem cell, the interstitial cell of hydra

Bode, Hans R.; David, Charles N.

doi:10.1016/0079-6107(79)90028-2

Cited by 94 publications

(32 citation statements)

References 57 publications

(52 reference statements)

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“…In the last few years much has been learned about the somatic differentiation potential of the interstitial stem cell population of the freshwater hydrozoan Hydra. Cell cloning experiments demonstrate the existence of a homogeneous population of stem cells that can give rise to both nerve cells and nematocytes (for a review see Bode and David, 1978). Recently, however, Littlefield et al (1985) and Littlefield (1985) have provided experimental evidence that in Hydra oligactis the interstitial cell population is not homogenous.…”

Section: Introductionmentioning

confidence: 99%

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Stem cells of Hydra magnipapillata can differentiate into somatic cells and germ line cells

Bosch

David

1987

Developmental Biology

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Section: Introductionmentioning

confidence: 99%

“…These cells include the class of and contains small tissue clumps and tentacle fragments. clone-forming cells (Bode and David, 1978).…”

Section: Preparation Of Stem Cell Clonesmentioning

confidence: 99%

Stem cells of Hydra magnipapillata can differentiate into somatic cells and germ line cells

Bosch

David

1987

Developmental Biology

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157

108

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“…Interstitial stem cells in hydra are multipotent cells which continuously differentiate nerve cells, nematocytes, and gland cells in asexual animals (see Bode and David, 1978;David et al, 1987 for reviews) and egg and sperm in sexual animals . Stem cells are distributed uniformly along the body column in the ectoderm; differentiation of nerve cells, nematocytes, and gland cells, however, is strongly localized in specific regions of the animal.…”

Section: Introductionmentioning

confidence: 99%

Cell cycle length, cell size, and proliferation rate in hydra stem cells

Holstein¹,

David²

1990

Developmental Biology

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We have analyzed the cell cycle parameters of interstitial cells in Hydra oligactis. Three subpopulations of cells with short, medium, and long cell cycles were identified. Short-cycle cells are stem cells; medium-cycle cells are precursors to nematocyte differentiation; long-cycle cells are precursors to gamete differentiation. We have also determined the effect of different cell densities on the population doubling time, cell cycle length, and cell size of interstitial cells. Our results indicate that decreasing the interstitial cell density from 0.35 to 0.1 interstitial cells/epithelial cell (1) shortens the population doubling time from 4 to 1.8 days, (2) increases the [SH]thymidine labeling index from 0.5 to 0.75 and shifts the nuclear DNA distribution from G2 to S phase cells, and (3) decreases the length of G2 in stem cells from 6 to 3 hr. The shortened cell cycle is correlated with a significant decrease in the size of interstitial stem cells. Coincident with the shortened cell cycle and increased growth rate there is an increase in stem cell self-renewal and a decrease in stem cell differentiation.

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“…These cells are uniformly distributed along the body column and can give rise to either nerve or nematocyte differentiation (for review see Bode and David, 1978). Commitment of stem cells to the nerve pathway occurs in the S phase of the stem cell cycle; committed cells complete the cell cycle and divide to yield differentiated nerve cells (Berking, 1979;Venugopal and David, 1981b;Yaross et al, 1982).…”

Section: Introductionmentioning

confidence: 99%