Regulation of alcoholic fermentation in batch and chemostat cultures ofKluyveromyces lactis CBS 2359

Kiers, Janine; Zeeman, Anne‐Marie; Luttik, Marijke A. H.; Thiele, Claudia; Castrillo, Juan I.; Steensma, H. Yde; Dijken, Johannes P. van; Pronk, Jack T.

doi:10.1002/(sici)1097-0061(19980330)14:5<459::aid-yea248>3.0.co;2-o

Cited by 119 publications

(146 citation statements)

References 42 publications

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“…This implies that the balance between respiration and fermentation in this yeast depends on oxygen availability and not on glucose concentration (Weirich et al, 1997;Kiers et al, 1998;González-Siso et al, 2000). The key point of the balance between respiration and fermentation is the branch point of pyruvate metabolism, with pyruvate being either oxidized by the respiratory enzyme pyruvate dehydrogenase (PDH) or decarboxylated by the fermentative enzyme pyruvate decarboxylase (PDC).…”

Section: Introductionmentioning

confidence: 80%

A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of the KlMGA2 gene in Kluyveromyces lactis

et al. 2012

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A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of the KlMGA2 gene in Kluyveromyces lactis In the respiratory yeast Kluyveromyces lactis, little is known about the factors regulating the metabolic response to oxygen shortage. After searching for homologues of characterized Saccharomyces cerevisiae regulators of the hypoxic response, we identified a gene that we named KlMGA2, which is homologous to MGA2. The deletion of KlMGA2 strongly reduced both the fermentative and respiratory growth rate and altered fatty acid composition and the unsaturation index of membranes. The reciprocal heterologous expression of MGA2 and KlMGA2 in the corresponding deletion mutant strains suggested that Mga2 and KlMga2 are functional homologues. KlMGA2 transcription was induced by hypoxia and the glucose sensor Rag4 mediated the hypoxic induction of KlMGA2. Transcription of lipid biosynthetic genes KlOLE1, KlERG1, KlFAS1 and KlATF1 was induced by hypoxia and was dependent on KlMga2, except for KlOLE1. Rag4 was required for hypoxic induction of transcription for both KlMga2-dependent (KlERG1) and KlMga2-independent (KlOLE1) structural genes.

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Section: Introductionmentioning

confidence: 80%

A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of the KlMGA2 gene in Kluyveromyces lactis

et al. 2012

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“…Examples are methanol-utilising yeasts such as Hansenula polymorpha and Pichia pastoris and the lactose-utilizing species Kluyveromyces lactis and marxianus (e.g. ␤-galactosidase and biomass production) [1][2][3]. It is accepted that the application of these yeasts in large scale fermentations for the production of heterologous proteins will be probably a common industrial practice by the end of this century [1].…”

Section: Introductionmentioning

confidence: 99%

Air pressure effects on biomass yield of two different Kluyveromyces strains

Pinheiro

Belo

Мота

2000

Enzyme and Microbial Technology

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The use of air pressure as a way of improving oxygen transfer in aerobic bioreactors was investigated. To compare the air pressure effects with traditional air bubbled cultures, experiments using a pressure reactor and a stirred flask, with the same oxygen transfer rate, were made. Kluyveromyces marxianus is an important industrial yeast and some of it show a "Kluyver effect" for lactose: even under oxygen limited growth conditions, certain disaccharides that support aerobic, respiratory growth, are not fermented. This study deals with the effect of increased pressure on the physiological behavior of two Kluyveromyces strains: K. marxianus ATCC10022 is a lactose-fermenting strain, whereas K. marxianus CBS 7894 has a Kluyver-effect for lactose. For K. marxianus ATCC10022 an air pressure increase of 2 bar led to a 3-fold increase in biomass yield. When air pressure increased an enhancement of ethanol oxidation of cell yeasts was also observed. Batch cultures of K. marxianus CBS 7894 exhibited different growth behaviour. Its metabolism was always oxidative and ethanol was never produced. With the increase in air pressure, it was possible to increase the productivity in biomass of K. marxianus CBS 7894. As a response to high oxygen concentrations, due to the increase in oxygen partial pressure, oxidative stress in the cells was also studied. Antioxidant defences, such as superoxide dismutase, catalase, and glutathione reductase, were at high activity levels, suggesting that these yeast strains could tolerate the increased pressures applied.

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“…K. marxianus cells showed significantly lower specific growth rate on ethanol when comparing to glucose (Table 2), which were practically the same values found for shake flask cultivation of K. lactis cells in both carbon sources (8). No effect on the cell growth was observed for lactose media.…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, Kluyveromyces lactis cells seem to use the Gs-Gogat pathway as the main glutamate biosynthetic pathway (12,14). Although this ATP-consuming pathway is energetically unfavourable, this could be related to the maintenance of redox equilibrium to reoxidise the excess of NADH produced during glycolysis, since ethanolic fermentation does not occur in this yeast under oxygen supply (8).…”

Section: Introductionmentioning

confidence: 99%

“…It has been related the biotechnological use of Kluyveromyces in the "Yeast as Cell Factory" concept to produce amino acidrich biomass due to its advantageous physiological characteristics compared to S. cerevisiae: the absence of ethanol production (Crabtree-negative effect), the lower catabolic repression response, the higher flux through the glycolytic pathway (8,15) and the high capacity of the respiratory enzymes (6,15 to produce bioingredients, such as oligopeptides, oligosacharides and oligonucleotides mainly for pharmaceutical and food industries (1). Similarly to K. lactis, K. marxianus is Crabtreenegative yeast, generally regarded as safe for human and animal consumption and possesses an arsenal of hydrolytic extracellular hydrolases that enables its use for bioconversion of complex sugar-rich substrates into single cell protein.…”

Section: Introductionmentioning

confidence: 99%

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The NADP+-dependent glutamate dehydrogenase of the yeast Kluyveromyces marxianus responds to nitrogen repression similarly to Saccharomyces cerevisiae

MoraisJunior¹

2003

Braz. J. Microbiol.

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NADP + -dependent glutamate dehydrogenase (NADP + -Gdh) is the first step in ammonia assimilation pathway in Saccharomyces cerevisiae and the knowledge of its regulation is the key for many biotechnological purposes such as single cell protein production. The regulation of NADP + -Gdh activity in Kluyveromyces marxianus cells was evaluated under different ammonia supply in batch cultivations. The results showed that K. marxianus NADP + -Gdh activity is induced over a narrow range of extracellular ammonia supply, being repressed by both high ammonia concentration and the glutamate formed. This activity is not growth-associated and may function mainly to trace low amounts of ammonia after growth cessation. The results demonstrated that NADP + -Gdh may not be the main enzyme for ammonia assimilation in K. marxianus, as it has been postulated for K. lactis, instead is subjected to the same regulatory mechanism described for S. cerevisiae.

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Regulation of alcoholic fermentation in batch and chemostat cultures ofKluyveromyces lactis CBS 2359

Cited by 119 publications

References 42 publications

A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of the KlMGA2 gene in Kluyveromyces lactis

A dual signalling pathway for the hypoxic expression of lipid genes, dependent on the glucose sensor Rag4, is revealed by the analysis of the KlMGA2 gene in Kluyveromyces lactis

Air pressure effects on biomass yield of two different Kluyveromyces strains

The NADP+-dependent glutamate dehydrogenase of the yeast Kluyveromyces marxianus responds to nitrogen repression similarly to Saccharomyces cerevisiae

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