Regulation of arginine biosynthesis, catabolism and transport in Escherichia coli

Charlier, Daniël; Bervoets, Indra

doi:10.1007/s00726-019-02757-8

Cited by 66 publications

(46 citation statements)

References 300 publications

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“…We previously performed a comprehensive HTS screen to map metabolic pathways that impacted virulence expression (with emphasis on LEE gene expression) in EHEC (4). This screen pulled ArgR, the arginine sensor that controls arginine utilization and biosynthesis, and the ArtP arginine importer (5,6), as potential regulators of LEE gene expression. Here we show that exogenous arginine enhances LEE and Stx expression in both EHEC and C. rodentium through the ArgR transcription factor (Figs.…”

Section: Discussionmentioning

confidence: 99%

“…To dissect the metabolic pathways that influence virulence of EHEC, we previously performed a high-throughput screen (HTS) that identified the arginine sensor ArgR, and the transmembrane arginine importer ArtP as possible regulators of EHEC virulence gene expression (4). ArgR is a transcription factor encoded by the argR gene that inhibits the transcription of multiple genes including the arginine biosynthesis regulon, the arginine transport genes, as well as its own transcription (5,6). ArgR also functions as a coactivator of the astCADBE operon encoding the arginine succinyltransferase pathway for arginine catabolism (7).…”

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confidence: 99%

“…L-Arginine acts as a precursor molecule for the biosynthesis of others amino acids, polyamines, and nitric oxide, and it also serves as a source of nitrogen upon its degradation via distinct catabolic routes (12). Arginine uptake in E. coli is mediated by three transport systems of the ATP-binding cassette transporter family (6,13). These import systems are encoded by two gene clusters, artPIQM-artJ and argT-hisJQMP, which are repressed by ArgR in E. coli.…”

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confidence: 99%

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l -Arginine sensing regulates virulence gene expression and disease progression in enteric pathogens

Menezes‐Garcia

Kumar

Zhu

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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Microbiota, host and dietary metabolites/signals compose the rich gut chemical environment, which profoundly impacts virulence of enteric pathogens. EnterohemorrhagicEscherichia coli(EHEC) engages a syringe-like machinery named type-III secretion system (T3SS) to inject effectors within host cells that lead to intestinal colonization and disease. We previously conducted a high-throughput screen to identify metabolic pathways that affect T3SS expression. Here we show that in the presence of arginine, the arginine sensor ArgR, identified through this screen, directly activates expression of the genes encoding the T3SS. Exogenously added arginine induces EHEC virulence gene expression in vitro. Congruently, a mutant deficient in arginine transport (ΔartP) had decreased virulence gene expression. ArgR also augments murine disease caused byCitrobacter rodentium, which is a murine pathogen extensively employed as a surrogate animal model for EHEC. The source of arginine sensed byC. rodentiumis not dietary. At the peak ofC. rodentiuminfection, increased arginine concentration in the colon correlated with down-regulation of the host SLC7A2 transporter. This increase in the concentration of colonic arginine promotes virulence gene expression inC. rodentium. Arginine is an important modulator of the host immune response to pathogens. Here we add that arginine also directly impacts bacterial virulence. These findings suggest that a delicate balance between host and pathogen responses to arginine occur during disease progression.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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l -Arginine sensing regulates virulence gene expression and disease progression in enteric pathogens

Menezes‐Garcia

Kumar

Zhu

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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“…Amino acids can serve as sources of carbon and nitrogen along with their role in protein synthesis. Arginine, in particular, plays additional vital roles in bacterial cell survival, such as its use in imparting acid resistance (Kanjee and Houry, 2013;Charlier and Bervoets, 2019) and as a substrate for the synthesis of the polyamines putrescine and spermidine (Igarashi and Kashiwagi, 2018;Charlier and Bervoets, 2019). With the increased transcription of arginine biosynthesis genes along with uptake of extracellular arginine demonstrated in our RNA-Seq results FIGURE 6 | Secretion of T cell-and DC-specific cytokines by polarized T84 cells.…”

Section: Discussionmentioning

confidence: 74%

Comparative Transcriptomics of Shiga Toxin-Producing and Commensal Escherichia coli and Cytokine Responses in Colonic Epithelial Cell Culture Infections

Harrison

Lacher

Mammel

et al. 2020

Front. Cell. Infect. Microbiol.

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“…Putrescine is produced from ornithine by either the SpeF or SpeC proteins, depending on the environmental condition, notably extracellular pH [ 36 ], or from agmatine by the SpeB protein ( Fig 1 ). The speB speC speF triple mutant displayed the lowest negative DNA supercoiling of the investigated E .…”

Section: Resultsmentioning

confidence: 99%

DNA supercoiling differences in bacteria result from disparate DNA gyrase activation by polyamines

Duprey

Groisman

2020

PLoS Genet

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DNA supercoiling is essential for all living cells because it controls all processes involving DNA. In bacteria, global DNA supercoiling results from the opposing activities of topoisomerase I, which relaxes DNA, and DNA gyrase, which compacts DNA. These enzymes are widely conserved, sharing >91% amino acid identity between the closely related species Escherichia coli and Salmonella enterica serovar Typhimurium. Why, then, do E . coli and Salmonella exhibit different DNA supercoiling when experiencing the same conditions? We now report that this surprising difference reflects disparate activation of their DNA gyrases by the polyamine spermidine and its precursor putrescine. In vitro , Salmonella DNA gyrase activity was sensitive to changes in putrescine concentration within the physiological range, whereas activity of the E . coli enzyme was not. In vivo , putrescine activated the Salmonella DNA gyrase and spermidine the E . coli enzyme. High extracellular Mg 2+ decreased DNA supercoiling exclusively in Salmonella by reducing the putrescine concentration. Our results establish the basis for the differences in global DNA supercoiling between E . coli and Salmonella , define a signal transduction pathway regulating DNA supercoiling, and identify potential targets for antibacterial agents.

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Regulation of arginine biosynthesis, catabolism and transport in Escherichia coli

Cited by 66 publications

References 300 publications

l -Arginine sensing regulates virulence gene expression and disease progression in enteric pathogens

l -Arginine sensing regulates virulence gene expression and disease progression in enteric pathogens

Comparative Transcriptomics of Shiga Toxin-Producing and Commensal Escherichia coli and Cytokine Responses in Colonic Epithelial Cell Culture Infections

DNA supercoiling differences in bacteria result from disparate DNA gyrase activation by polyamines

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