Regulation of ascorbate-glutathione cycle by exogenous nitric oxide and hydrogen peroxide in soybean roots under arsenate stress

Chromium toxicity to crops is a major scientific problem of the present time. Thus, scientific attempts have been made for reducing chromium toxicity to crop plants.• In this study, we examined the potential of ethylene (ET, 25 µM) and hydrogen sulphide (H 2 S, 10 µM) to alleviate hexavalent chromium [Cr(VI), 50 µM] stress in two pulse crops, black bean and mung bean, by assessing physiological and biochemical attributes.• Cr(VI) reduced shoot and root length in black bean and mung bean in comparison to the control. Plants had increased accumulation of oxidative stress markers, i.e. superoxide radicals (SOR), hydrogen peroxide (H 2 O 2 ) and lipid peroxidation (as malondialdehyde, MDA). The addition of AVG (an inhibitor of ET biosynthesis) and PAG (an inhibitor of H 2 S biosynthesis) to Cr(VI)-treated plants further increased Cr(VI) toxicity, suggesting their endogenous levels are important for tolerating Cr(VI) toxicity. However, supplementation with either ET or H 2 S alleviated Cr(VI) toxicity. Interestingly, ET did not rescue negative effects of PAG under Cr(VI) stress but NaHS rescued negative effect of AVG.• Overall, results indicate that, although both ET and H 2 S alleviate Cr(VI) stress, endogenous H 2 S is better. Furthermore, H 2 S appears to be a downstream signal for ET in alleviating Cr(VI) stress in these two pulse crops

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“…(2020) in rice under herbicide stress and Singh et al . (2021) in tomato and aubergine seedlings under Cr(VI) stress.…”

Section: Resultsmentioning

confidence: 99%

Ethylene and hydrogen sulphide are essential for mitigating hexavalent chromium stress in two pulse crops

et al. 2021

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“…Previous studies have shown that NO, an important endogenous gas signaling molecule, is involved in almost all biological processes in plants, including seed germination (Wimalasekera et al, 2011), plant maturation, and senescence (Guo and Crawford, 2005;Mishina et al, 2007). Additionally, NO has been shown to regulate numerous plant responses to a variety of biotic and abiotic stresses and to relieve the damage caused by oxidative stress (Siddiqui et al, 2011;Ahanger et al, 2020;Kaya et al, 2020b,c;Singh et al, 2020). According to previous studies, some shared physiological functions of MT and NO have been verified in their regulation of plant tolerance to stress conditions; in particular, both are involved in ROS signaling pathways in plants (Park et al, 2013;Scheler et al, 2013;Kaya et al, 2019Kaya et al, , 2020a.…”

Section: Introductionmentioning

confidence: 99%

Nitric Oxide Functions as a Downstream Signal for Melatonin-Induced Cold Tolerance in Cucumber Seedlings

Feng

Lu-jie

et al. 2021

Front. Plant Sci.

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Melatonin (MT) and nitric oxide (NO) are two multifunctional signaling molecules that are involved in the response of plants to abiotic stresses. However, how MT and NO synergize in response to cold stress affecting plants is still not clear. In this study, we found that endogenous MT accumulation under cold stress was positively correlated with cold tolerance in different varieties of cucumber seedlings. The data presented here also provide evidence that endogenous NO is involved in the response to cold stress. About 100 μM MT significantly increased the nitrate reductase (NR) activity, NR-relative messenger RNA (mRNA) expression, and endogenous NO accumulation in cucumber seedlings. However, 75 μM sodium nitroprusside (SNP, a NO donor) showed no significant effect on the relative mRNA expression of tryptophan decarboxylase (TDC), tryptamine-5-hydroxylase (T5H), serotonin-N-acetyltransferase (SNAT), or acetylserotonin O-methyltransferase (ASMT), the key genes for MT synthesis and endogenous MT levels. Compared with H2O treatment, both MT and SNP decreased electrolyte leakage (EL), malondialdehyde (MDA), and reactive oxygen species (ROS) accumulation by activating the antioxidant system and consequently mitigated cold damage in cucumber seedlings. MT and SNP also enhanced photosynthetic carbon assimilation, which was mainly attributed to an increase in the activity and mRNA expression of the key enzymes in the Calvin–Benson cycle. Simultaneously, MT- and SNP-induced photoprotection for both photosystem II (PSII) and photosystem I (PSI) in cucumber seedlings, by stimulating the PsbA (D1) protein repair pathway and ferredoxin-mediated NADP+ photoreduction, respectively. Moreover, exogenous MT and SNP markedly upregulated the expression of chilling response genes, such as inducer of CBF expression (ICE1), C-repeat-binding factor (CBF1), and cold-responsive (COR47). MT-induced cold tolerance was suppressed by 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO, a specific scavenger of NO). However, p-chlorophenylalanine (p-CPA, a MT synthesis inhibitor) did not affect NO-induced cold tolerance. Thus, novel results suggest that NO acts as a downstream signal in the MT-induced plant tolerance to cold stress.

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“…1d). H 2 O 2 and NO might be inducing the protein biosynthesis by controlling the ROS metabolism inside the cell 26 . Interestingly, ROS production was further induced (Table 1) by supplementation of PTIO and L NAME that resulted to the damage in protein content (Fig.…”

Section: Discussionmentioning

confidence: 99%

Regulation of redox homeostasis in cadmium stressed rice field cyanobacteria by exogenous hydrogen peroxide and nitric oxide

Verma

Prasad

2021

Sci Rep

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In the present study, defensive strategies of H2O2 mediated NO signaling were analyzed in Cd stressed Nostoc muscorum and Anabaena sp. Exogenously supplied SNP (10 µM) and H2O2 (1 µM) lessen the toxicity of Cd (6 µM) but without NO; H2O2 was unable to release the stress from cyanobacterial cells potentially. The reduced contents of exopolysaccharide, protein content, endogenous NO and enzymatic antioxidants (SOD, POD, CAT, and GST) due to Cd toxicity, were found increased significantly after exogenous application of H2O2 and SNP thereafter, cyanobacterial calls flourished much better after releasing toxic level of Cd. Moreover, increased level of ROS due to Cd stress also normalized under exogenous application of H2O2 and SNP. However, chelation of NO hindered the signaling mechanism of H2O2 that diminished its potential against Cd stress while signaling of NO has not been hindered by chelation of H2O2 and NO potentially released the Cd stress from cyanobacterial cells. In conclusion, current findings demonstrated the synergistic signaling between H2O2 and NO towards the improvement of cyanobacterial tolerance to Cd stress, thereby enhancing the growth and antioxidant defense system of test cyanobacteria that improved fertility and productivity of soil even under the situation of metal contamination.

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Regulation of ascorbate-glutathione cycle by exogenous nitric oxide and hydrogen peroxide in soybean roots under arsenate stress

Cited by 69 publications

References 78 publications

Ethylene and hydrogen sulphide are essential for mitigating hexavalent chromium stress in two pulse crops

Ethylene and hydrogen sulphide are essential for mitigating hexavalent chromium stress in two pulse crops

Nitric Oxide Functions as a Downstream Signal for Melatonin-Induced Cold Tolerance in Cucumber Seedlings

Regulation of redox homeostasis in cadmium stressed rice field cyanobacteria by exogenous hydrogen peroxide and nitric oxide

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