Regulation of biofilm formation by marT in Salmonella Typhimurium

Eran, Zeynep; Akçelik, Mustafa; Yazıcı, Betül Cansu; Özcengiz, Gülay; Akçelik, Nefise

doi:10.1007/s11033-020-05573-6

Cited by 16 publications

(9 citation statements)

References 34 publications

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“…Both curli fimbrae and cellulose synthesis are co-regulated through the gene csgD. Thus, the inhibition of expression of csgD genes will inhibit cellulose production through the adrA regulator gene [26,40], which is consistent with the decrease in or absence of exo-polysaccharide matrix observed in SEM images (Figure 3b-d). Additionally, EOs inhibited the gene expression of motB, fliz, and flhD, related to cell motility, reducing the bacterial capacity to produce biofilm.…”

Section: Plant Materialssupporting

confidence: 77%

Effect of Essential Oils on the Inhibition of Biofilm and Quorum Sensing in Salmonella enteritidis 13076 and Salmonella typhimurium 14028

Guillín

Cáceres

Torres³

et al. 2021

Antibiotics

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The emergence of multidrug-resistant microorganisms represents a global challenge that has led to a search for new antimicrobial compounds. Essential oils (EOs) from medicinal aromatic plants are a potential alternative for conventional antibiotics. In this study, the antimicrobial and anti-biofilm potential of 15 EOs was evaluated on planktonic and biofilm-associated cells of Salmonella enterica serovar Enteritidis ATCC 13076 (S. enteritidis) and Salmonella enterica serovar Typhimurium ATCC 14028 (S. typhimurium). In total, 4 out of 15 EOs showed antimicrobial activity and 6 EOs showed anti-biofilm activity against both strains. The EO from the Lippia origanoides chemotype thymol-carvacrol II (LTC II) presented the lowest minimum inhibitory concentration (MIC50 = 0.37 mg mL−1) and minimum bactericidal concentration (MBC = 0.75 mg mL−1) values. This EO also presented the highest percentage of biofilm inhibition (>65%) on both microorganisms, which could be confirmed by scanning electron microscopy (SEM) images. Transcriptional analysis showed significant changes in the expression of the genes related to quorum sensing and the formation of the biofilm. EOs could inhibit the expression of genes involved in the quorum sensing mechanism (luxR, luxS, qseB, sdiA) and biofilm formation (csgA, csgB, csgD, flhD, fliZ, and motB), indicating their potential use as anti-biofilm antimicrobial agents. However, further studies are needed to elucidate the action mechanisms of essential oils on the bacterial cells under study.

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Section: Plant Materialssupporting

confidence: 77%

Effect of Essential Oils on the Inhibition of Biofilm and Quorum Sensing in Salmonella enteritidis 13076 and Salmonella typhimurium 14028

Guillín

Cáceres

Torres³

et al. 2021

Antibiotics

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“…The formation of S. Typhimurium biofilms is not determined by a single factor but by many factors forming a complex regulatory network. Jonas et al studied the joint regulation of Csr protein, the second messenger c-di-GMP and bacterial motility on the biofilm of S. Typhimurium [ 22 ].Eran et al studied genes related to biofilm formation [ 23 ]. The Mar T gene positively regulates the expression of 14 genes, which are related to the formation of biofilms.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome sequencing reveals the difference in the expression of biofilm and planktonic cells between two strains of Salmonella Typhimurium

Zheng

Zhang

et al. 2022

Biofilm

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“…Moreover, ST can survive in stressful environments such as low temperature, hyperosmolarity, and acidity, and form biofilms under certain conditions to resist unfavorable internal and external environments ( Kim et al 2013 ; Ramachandran et al 2016 ). It has been proven that many factors are associated with the responses of Salmonella Typhimurium to environmental stress and biofilm formation ( Eran et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

A Novel LysR Family Factor STM0859 is Associated with The Responses of Salmonella Typhimurium to Environmental Stress and Biofilm Formation

Ning

et al. 2021

Polish Journal of Microbiology

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Salmonella enterica subsp. enterica serovar Typhimurium (ST) is an intracellularly parasitic bacterium. This zoonotic pathogen causes food poisoning and thus imposes a severe threat to food safety. Here, to understand the regulatory roles of the novel transcription factor STM0859 on the response of ST to environmental stress and biofilm formation, the STM0859 gene-deficient strain and the complementation strain ΔSTM0859/STM0859 were generated, respectively. Then, its capacity of responding to environmental stresses and biofilm (BF) formation ability under different stresses, including acid, alkali, high salt, cholate, and oxidative stresses was tested. We further analyzed the interaction between the STM0859 protein and the promoter of the acid stress response-related gene rcsB by performing an electrophoresis mobility shift assay (EMSA). The results showed that acid resistance and BF formation capacities of ST-ΔSTM0859 strain were significantly weaker, as compared with those of Salmonella Typhimurium SL1344 (ST-SL1344) wild strain (p < 0.01). Quantitative qRT-PCR analysis showed that the expression levels of acid stress and BF formation-related genes, rcsB and rpoS, of ST-ΔSTM0859 strain were significantly reduced at the transcription levels, while the transcription levels of these genes were fully restored in complementation strain ST-ΔSTM0859/STM0859. The results of EMSA showed that STM0859 was capable of binding the promoter DNA fragments of the rcsB gene, suggesting that STM0859 can promote the transcription of the rcsB gene through interaction with its promoter, thereby exerting an indirectly regulatory role on the adaptive responses to acid stress and BF formation of ST. This study provided new insights into the regulatory mechanisms of the LysR family factors on the tolerances of ST under adverse environmental stresses.

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Regulation of biofilm formation by marT in Salmonella Typhimurium

Cited by 16 publications

References 34 publications

Effect of Essential Oils on the Inhibition of Biofilm and Quorum Sensing in Salmonella enteritidis 13076 and Salmonella typhimurium 14028

Effect of Essential Oils on the Inhibition of Biofilm and Quorum Sensing in Salmonella enteritidis 13076 and Salmonella typhimurium 14028

Transcriptome sequencing reveals the difference in the expression of biofilm and planktonic cells between two strains of Salmonella Typhimurium

A Novel LysR Family Factor STM0859 is Associated with The Responses of Salmonella Typhimurium to Environmental Stress and Biofilm Formation

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